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驴 anti-人 IgG Antibody (Cy5.5) - Preadsorbed

FACS, FLISA, FM Polyclonal IgG Cy5.5
产品编号 ABIN6698912
发货至: 中国
  • 抗原 See all IgG products
    IgG
    适用
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    克隆类型
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    多克隆
    标记
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    Cy5.5
    应用范围
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    Flow Cytometry (FACS), FLISA, Fluorescence Microscopy (FM)
    产品特性

    Synonyms: Donkey Anti Human IgG CY5.5 Conjugated Antibody, Donkey Anti-Human IgG Antibody CY5.5 Conjugation

    Background: Anti-Human IgG (H&L) Cy5.5 generated in donkey detects human Immunoglobulin G (heavy and light chains). It is a protein complex composed of four peptide chains - two identical heavy chains and two identical light chains arranged in a Y-shape typical of antibody monomers. Each IgG has two antigen binding sites. Representing approximately 75 % of serum immunoglobulins in humans, IgG is the most abundant antibody isotype found in the circulation. IgG molecules are synthesized and secreted by plasma B cells. Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition.

    纯化方法
    Preadsorption: Solid phase absorption
    免疫原

    Immunogen: Human IgG whole molecule

    亚型
    IgG
  • 应用备注

    Application Note: This product is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.

    FLISA Dilution: 1:10,000 - 1:50,000

    Flow Cytometry Dilution: 1:500 - 1:2,500

    IF Microscopy Dilution: 1:1,000 - 1:5,000

    限制
    仅限研究用
  • 状态
    Lyophilized
    溶解方式

    Reconstitution Volume: 1.0 mL

    Reconstitution Buffer: Restore with deionized water (or equivalent)

    缓冲液
    0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
    储存条件
    RT,4 °C,-20 °C
    储存方法
    Store vial at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use. 
    有效期
    12 months
  • 抗原
    IgG
    Abstract
    IgG 产品
    物质类
    Antibody
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