山羊 anti-人 IgG Antibody (Cy3.5) - Preadsorbed
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- 抗原 See all IgG products
- IgG
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适用
- 人
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宿主
- 山羊
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克隆类型
- 多克隆
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标记
- Cy3.5
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应用范围
- Flow Cytometry (FACS), FLISA, Fluorescence Microscopy (FM)
- 产品特性
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Synonyms: Goat Anti Human IgG CY3 Conjugated Antibody, Goat Anti-Human IgG Antibody CY3 Conjugation
Background: Anti-Human IgG (H&L) Cy3.5 generated in goat detects human Immunoglobulin G (heavy and light chains). It is a protein complex composed of four peptide chains - two identical heavy chains and two identical light chains arranged in a Y-shape typical of antibody monomers. Each IgG has two antigen binding sites. Representing approximately 75 % of serum immunoglobulins in humans, IgG is the most abundant antibody isotype found in the circulation. IgG molecules are synthesized and secreted by plasma B cells. Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition.
- 纯化方法
- Preadsorption: Solid phase absorption
- 免疫原
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Immunogen: Human IgG whole molecule
- 亚型
- IgG
- Labeling Ratio
- 2.9
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Mouse anti-Human IgG (Fab Region) antibody
ELISA, WB, FACS Monoclonal 4A11 IgG2b unconjugated
Mouse anti-Human IgG (Fc Region) antibody (PE)ELISA, FACS Monoclonal JDC-10 IgG1 PE
Mouse anti-Human IgG (Fab Region) antibody (PE)FACS Monoclonal 4A11 IgG2b PE
Goat anti-Human IgG antibody (PE)ELISA, FACS Polyclonal IgG PE F(ab')2 fragment
Mouse anti-Human IgG (Fc Region) antibody (APC)ELISA, FACS Monoclonal H2 IgG2b APC
Goat anti-Human IgG (Fc Region) antibody (PE) - PreadsorbedFACS, FM Polyclonal IgG PE F(ab')2 fragment
Goat anti-Human IgG (Fc Region) antibody (Alkaline Phosphatase (AP))ELISA, FLISA Polyclonal IgG Alkaline Phosphatase (AP)
Goat anti-Human IgG (Fd Region) antibody (FITC)ELISA, FLISA Polyclonal IgG FITC
Goat anti-Human IgG antibody (HRP) - PreadsorbedELISA, FACS, FLISA Polyclonal IgG HRP F(ab')2 fragment
Goat anti-Human IgG (Fd Region) antibody (HRP)ELISA, FLISA Polyclonal IgG HRP
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- 应用备注
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Application Note: This product is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.
FLISA Dilution: 1:10,000 - 1:50,000
Flow Cytometry Dilution: 1:500 - 1:2,500
IF Microscopy Dilution: 1:1,000 - 1:5,000
- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 溶解方式
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Reconstitution Volume: 1.0 mL
Reconstitution Buffer: Restore with deionized water (or equivalent)
- 缓冲液
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
0.01 % (w/v) Sodium Azide - 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- RT,4 °C,-20 °C
- 储存方法
- Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
- 有效期
- 12 months
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- 抗原
- IgG
- Abstract
- IgG 产品
- 物质类
- Antibody
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