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Fibrillarin 抗体

FBL 适用: Saccharomyces cerevisiae WB, FM 宿主: 小鼠 Monoclonal 38F3 unconjugated
产品编号 ABIN6658299
发货至: 中国
  • 抗原 See all Fibrillarin (FBL) 抗体
    Fibrillarin (FBL)
    适用
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    Saccharomyces cerevisiae
    宿主
    • 39
    • 10
    • 1
    小鼠
    克隆类型
    • 38
    • 12
    单克隆
    标记
    • 35
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    This Fibrillarin antibody is un-conjugated
    应用范围
    • 48
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    Western Blotting (WB), Fluorescence Microscopy (FM)
    交叉反应
    人, 小鼠, 大鼠
    纯化方法
    Anti-Fibrillarin Antibody is directed against yeast Fibrillarin/Nop1p protein. Fibrillarin antibodies are protein G purified from concentrated clarified cell culture supernate. Reactivity is expected against human, mouse, and rat. Cross-reactivity toward other sources has not be determined. Anti-Fibrillarin antibody is becoming widely used as a convenient marker for nucleoli in a wide variety of species.
    免疫原

    Immunogen: Anti-Fibrillarin Monoclonal Antibody was produced in mouse by repeated immunizations with native yeast nuclear protein preparations.

    Immunogen Type: Native Protein

    克隆位点
    38F3
    亚型
    IgG
    Top Product
    Discover our top product FBL Primary Antibody
  • 应用备注

    Application Note: Anti-Fibrillarin (Mouse) Antibody is suitable for use in Western Blotting and IF. Specific conditions for reactivity should be optimized by the end user. Expect a band size of approximately 34 kDa in size corresponding to the Fibrillarin/Nop1p proteins.

    Western Blot Dilution: 1:1000

    IF Microscopy Dilution: 1:500

    限制
    仅限研究用
  • 状态
    Liquid
    缓冲液

    Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

    Stabilizer: 50 % (v/v) Glycerol

    0.01 % (w/v) Sodium Azide
    储存液
    Sodium azide
    注意事项
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    储存条件
    4 °C,-20 °C
    储存方法
    Store vial at -20° C prior to opening.  This product is stable at 4° C as an undiluted liquid.  For extended storage, aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing. Dilute only prior to immediate use.
  • 抗原
    Fibrillarin (FBL)
    别名
    Fibrillarin (FBL 产品)
    别名
    FIB antibody, FLRN antibody, RNU3IP1 antibody, wu:fb37g12 antibody, zgc:56145 antibody, zgc:77130 antibody, FBL antibody, AL022665 antibody, CG9888 antibody, Dmel\\CG9888 antibody, Fibri antibody, GCR-6 antibody, GCR6 antibody, Pen59C5 antibody, fib antibody, pen59C5 antibody, MGC76139 antibody, fbl antibody, fibrillarin antibody, Fibrillarin antibody, putative fibrillarin antibody, fibrillarin S homeolog antibody, FBL antibody, fbl antibody, Fbl antibody, Fib antibody, fib1 antibody, LMJF_19_0100 antibody, fbl.S antibody
    背景

    Synonyms: mouse anti-Fibrillarin Antibody, rRNA 2'-O-methyltransferase fibrillarin, U3 small nucleolar RNA-associated protein NOP1, Nucleolar protein 1, U3 snoRNA-associated protein NOP1

    Background: Fibrillarin Antibody detects Nop1p which was originally identified as a nucleolar protein of bakers yeast, Saccharomyces cerevisiae. The Nop1p protein is 327 amino acids in size (34.5 kDa), is essential for yeast viability, and is localized in the nucleoli. The systematic name for S. cerevisiae Nop1 is YDL014W, and it is now known to be part of the small subunit processome complex, involved in the processing of pre-18S ribosomal RNA. Nop1p is the yeast homologue of a protein found in all eukaryotes and archea generally called fibrillarin. Fibrillarin/Nop1p is extraordinarily conserved, so that the yeast and human proteins are 67 % identical, and the human protein can functionally replace the yeast protein. Patients with the autoimmune disease scleroderma often have strong circulating autoantibodies to a ~34 kDa protein which was subsequently found to be fibrillarin. Recent studies show that knock-out of the fibrillarin gene in mice results in embryonic lethality, although mice with only one functional fibrillarin/Nop1p gene were viable. Anti-Fibrillarin Antibody is ideal for investigators involved in Cell Proliferation and Cancer Research.

    Gene Name: NOP1

    基因ID
    851548
    UniProt
    P15646
    途径
    Ribonucleoside Biosynthetic Process
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