我们推荐在您的浏览器上开启Javascript，以获得最佳效果。

DAB1 抗体 (C-Term)

Name: DAB1 抗体 (C-Term)
SKU: ABIN6658036
Availability: OutOfStock

DAB1 适用: 小鼠, 兔 WB, ELISA, IHC, IP 宿主: 兔 Polyclonal unconjugated

WB - Dab1 Antibody Analysis of GFP-Dab1 and endogenous Dab1 levels in transfected retinal cells and retinal tissue.

IF - Dab1 Antibody Immunohistochemical staining after cryofixation and sectioning of mouse brain tissue using (A) a 1:5,000 dilution of anti-Dab-1 and (B) 1:5,000 dilution of pre-immune serum followed by processing with HRP Goat anti-Rabbit IgG [H&L] and chromogenic substrate.

WB - Dab1 Antibody Immunochemical's anti-Dab1 is shown to detect Dab1 present in wt mouse brain extracts (lane 1).

3 images

(1 reference)

产品编号 ABIN6658036

发货至: 中国

Datasheet as PDF

抗原 See all DAB1 抗体

DAB1 (Disabled Homolog 1 (Drosophila) (DAB1))
抗原表位
22

15

15

7

5

3

3

3

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
AA 400-555, C-Term
适用
62

61

60

4

3

3

3

3

3

2

2

1
小鼠, 兔
宿主
94

4

3
兔
Compatible Secondaries
克隆类型
100

1
多克隆
标记
45

6

5

4

4

4

4

4

4

4

3

3

3

3

3

1

1
This DAB1 antibody is un-conjugated
应用范围
70

41

41

34

22

17

11

9

6

6

2

1

1
Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunoprecipitation (IP)

交叉反应

小鼠

纯化方法

Dab-1 whole rabbit antiserum was prepared by delipidation and defibrination followed by the addition of buffer salts and preservative. This antibody is directed against Dab1 from mouse. Cross-reactivity with other species has not been determined. No reaction occurs with human or mouse Dab2.

免疫原

Immunogen: This whole rabbit serum was prepared by repeated immunizations with a synthetic peptide corresponding to the C-terminal region of murine Dab1 at amino acids 400-555.
Immunogen Type: Peptide

Top Product

Discover our top product DAB1 Primary Antibody
anti-Disabled Homolog 1 (Drosophila) (DAB1) (pTyr232) antibody
DAB1 适用: 人, 小鼠, 大鼠 WB, ELISA, IF, ICC 宿主: 兔 Polyclonal unconjugated

anti-Disabled Homolog 1 (Drosophila) (DAB1) (AA 400-555), (C-Term) antibody
DAB1 适用: 小鼠 WB, ELISA, IHC, IP 宿主: 兔 Polyclonal unconjugated

anti-Disabled Homolog 1 (Drosophila) (DAB1) (pTyr232) antibody
DAB1 适用: 人 WB, ELISA, IF (cc), IF (p), IHC (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated

anti-Disabled Homolog 1 (Drosophila) (DAB1) (Ser296) antibody
DAB1 适用: 人, 小鼠, 大鼠 WB, ELISA, IF 宿主: 兔 Polyclonal unconjugated

anti-Disabled Homolog 1 (Drosophila) (DAB1) (AA 1-185) antibody
DAB1 适用: 人 WB, IF 宿主: 兔 Polyclonal unconjugated

anti-Disabled Homolog 1 (Drosophila) (DAB1) (pTyr198) antibody
DAB1 适用: 大鼠 WB, ELISA, IF (cc), IF (p), IHC (p), IHC (fro), ICC 宿主: 兔 Polyclonal unconjugated

anti-Disabled Homolog 1 (Drosophila) (DAB1) antibody
DAB1 适用: 人 ELISA, IHC 宿主: 兔 Polyclonal unconjugated

anti-Disabled Homolog 1 (Drosophila) (DAB1) (Ser295) antibody
DAB1 适用: 人, 小鼠, 大鼠 WB, ELISA, IF 宿主: 兔 Polyclonal unconjugated

anti-Disabled Homolog 1 (Drosophila) (DAB1) (AA 187-236) antibody
DAB1 适用: 人, 小鼠, 大鼠 WB, ELISA, IF, IHC (p), IHC 宿主: 兔 Polyclonal unconjugated

anti-Disabled Homolog 1 (Drosophila) (DAB1) (AA 1-553) antibody
DAB1 适用: 人 WB 宿主: 小鼠 Polyclonal unconjugated

应用备注

Immunohistochemistry Dilution: 1:5,000
Application Note: Anti-Dab1 antibody is suitable for the detection of Dab1 by immunoprecipitation, immunohistochemistry and western blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band at 80 kDa corresponding to Dab1 in the appropriate tissue extract or cell lysate. For western blotting block the blot using 5 % BLOTTO for 1 h at room temperature and followed by incubation with the primary antibody diluted in 1 % BLOTTO in TTBS for 1 h at room temperature. For immunoprecipitation use 1 μL of antiserum per 500 μg of brain lysate. Perform immunoprecipitation at 4 °C for 2 h. For immunoprecipitation buffer lysates with 50 mM Tris-Cl, pH 7.4, supplemented with 150 mM sodium chloride, 1 % (v/v) NP-40, 10 μg/mL aprotinin and 10 μg/mL leupeptin.
Immunoprecipitation Dilution: 1 μL per 500 μg
ELISA Dilution: 1:10,000 - 1:50,000
Western Blot Dilution: 1:5,000

限制

仅限研究用
状态

Liquid

缓冲液

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
0.01 % (w/v) Sodium Azide
Stabilizer: None

储存液

Sodium azide

注意事项

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

储存条件

-20 °C

储存方法

Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 μL). To minimize loss of volume dilute 1:10 by adding 225 μL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.
Katyal, Gao, Monckton, Glubrecht, Godbout: "Hierarchical disabled-1 tyrosine phosphorylation in Src family kinase activation and neurite formation." in: Journal of molecular biology, Vol. 368, Issue 2, pp. 349-64, (2007) (PubMed).
抗原

DAB1 (Disabled Homolog 1 (Drosophila) (DAB1))

别名

Dab1 (DAB1 产品)

别名

AI956902 antibody, C630028C02Rik antibody, scm antibody, scr antibody, scrambler antibody, yot antibody, disabled-1 antibody, LOC100232823 antibody, si:dkey-242m13.1 antibody, DAB1 antibody, zgc:136547 antibody, DAB1, reelin adaptor protein antibody, disabled 1 antibody, disabled homolog 1 antibody, Dab, reelin signal transducer, homolog 1a (Drosophila) antibody, Dab, reelin signal transducer, homolog 1b (Drosophila) antibody, DAB1 antibody, Dab1 antibody, LOC100232823 antibody, dab1a antibody, dab1b antibody

背景

Synonyms: rabbit anti-Dab 1 antibody, Disabled homolog 1 antibody, Disabled homolog 1 Drosophila antibody, Scm antibody, Scr antibody, Scrambler antibody, Yot antibody, Yotari antibody
Background: Anti-Dab1 Antibody recognizes Dab1 that is a phosphoprotein encoded by the mouse gene dab and is related to the Drosophila gene 'disabled'. Mutations in the mouse dab gene may result in the 'scrambler' and 'yotari' phenotypes. Dab1 binds to non-receptor tyrosine kinases and plays an important role in brain development. Dab1 is expressed in neuronal populations exposed to reelin, and it functions as a signaling molecule that regulates cell positioning in the developing brain. Cloning of human Dab1 and sequence determinations suggest a 96 % identity to the mouse sequence. Dab1 binds to cytoplasmic regions of very low density lipoprotein receptors (VLDLR), apolipoprotein E receptor-2 (ApoER2) and the Amyloid Precursor Protein (APP) family of proteins. Dab1 accumulates in ectopic neurons from mice lacking Reelin or both VLDLR and ApoER2. In humans, Dab1 has been mapped to lp32-p312. This region shows homology of synteny with the segment of mouse chromosome 4 containing Dab1.
Gene Name: DAB1

基因ID

1600, 33350928

UniProt

O75553