TJP1 抗体
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- 抗原 See all TJP1 抗体
- TJP1 (Tight Junction Protein 1 (TJP1))
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适用
- 人
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宿主
- 兔
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克隆类型
- 多克隆
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标记
- This TJP1 antibody is un-conjugated
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应用范围
- Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Flow Cytometry (FACS), Fluorescence Microscopy (FM)
- 交叉反应
- 人, 小鼠
- 纯化方法
- This affinity purified antibody is directed against human ZO-1. This product was affinity purified from monospecific antiserum by immunoaffinity purification. Blast analysis of the sequence of the immunogen shows 100% identity with human.
- 免疫原
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Immunogen: Anti-ZO-1 antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal portion of human ZO-1 conjugated to Keyhole Limpet Hemocyanin (KLH).
Immunogen Type: Peptide
- 亚型
- IgG
- Top Product
- Discover our top product TJP1 Primary Antibody
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anti-Tight Junction Protein 1 (TJP1) (AA 1551-1702) antibody
TJP1 适用: 人, 小鼠, 大鼠, Pig, Cow WB, ELISA, FACS, IHC (p), IF (p), IF (cc), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Tight Junction Protein 1 (TJP1) (C-Term) antibodyTJP1 适用: 人, 小鼠, 大鼠, Pig, Cow, 犬, 豚鼠, 兔, 马, 猴, 小鸡, Avian, Cat, 驴, 山羊, Hamster, 绵羊 WB, IF, IHC (p), IHC (fro) 宿主: 山羊 Polyclonal unconjugated
anti-Tight Junction Protein 1 (TJP1) (N-Term) antibodyKO Validated TJP1 适用: 人 WB, IP, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Tight Junction Protein 1 (TJP1) (Internal Region) antibodyVerified TJP1 适用: 人 ELISA, IF 宿主: 山羊 Polyclonal unconjugated
anti-Tight Junction Protein 1 (TJP1) (AA 1600-1700) antibodyTJP1 适用: 人 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Tight Junction Protein 1 (TJP1) antibodyTJP1 适用: 人 WB, IHC, ELISA, FACS, FM 宿主: 兔 Polyclonal unconjugated
anti-Tight Junction Protein 1 (TJP1) antibodyTJP1 适用: 人, 小鼠, 大鼠, Pig WB, IHC, ELISA, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Tight Junction Protein 1 (TJP1) (AA 1178-1527) antibodyTJP1 适用: 人, 小鼠, 大鼠 WB, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Tight Junction Protein 1 (TJP1) antibodyTJP1 适用: 小鼠 WB, IHC, IP 宿主: 大鼠 Monoclonal unconjugated
anti-Tight Junction Protein 1 (TJP1) (AA 1-190) antibodyTJP1 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
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- 应用备注
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Immunohistochemistry Dilution: 1:100 - 1:200
Application Note: Anti-ZO-1 Antibody has been tested in Western Blot, ELISA, Immunohistochemistry, Immunofluorescence, and Flow Cytometry. Expect a band at ~245 and/or 195.5 kDa in western blot using appropriate lysates. Positive control whole cell lysates used A549 and PC3 @ 1 μg/mL for WB, CACO2 and PC3 with PFA and MeOH @ 10 μg/mL for IF. Positive control cells for FC were PC3 and positive control tissues for IHC was mouse adipose tissue.
ELISA Dilution: 5 μg/mL
Flow Cytometry Dilution: User Optimized
Western Blot Dilution: 1:1000
IF Microscopy Dilution: 10 μg/mL
- 限制
- 仅限研究用
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- 状态
- Liquid
- 缓冲液
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
0.01 % (w/v) Sodium AzideStabilizer: None
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- -20 °C
- 储存方法
- Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 μL). To minimize loss of volume dilute 1:10 by adding 225 μL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.
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- 抗原
- TJP1 (Tight Junction Protein 1 (TJP1))
- 别名
- ZO-1 (TJP1 产品)
- 别名
- ZO-1 antibody, ZO1 antibody, zo1 antibody, tight junction protein 1 antibody, tight junction protein ZO-1 antibody, TJP1 antibody, Tjp1 antibody, LOC396567 antibody
- 背景
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Synonyms: rabbit anti-ZO-1 antibody, ZO 1, ZO1, Tight junction protein ZO-1, Tight junction protein 1, Zona occludens protein 1, Zonula occludens protein 1, TJP1
Background: ZO-1, also called TJP1, belongs to the MAGUK family. This gene encodes a protein located on a cytoplasmic membrane surface of intercellular tight junctions. The encoded protein may be involved in signal transduction at cell-cell junctions. The N-terminal may be involved in transducing a signal required for tight junction assembly, while the C-terminal may have specific properties of tight junctions. The alpha domain might be involved in stabilizing junctions. ZO-1 plays a role in the regulation of cell migration by targeting CDC42BPB to the leading edge of migrating cells. ZO1 may be associated the following disorders, celiac desease, congenital nephrotic syndrome finnish type, and macular degeneration. Anti-ZO-1 Antibody is useful for researchers interested in Apoptosis Research and Insulin Research.
Gene Name: TJP1
- 基因ID
- 7082
- NCBI登录号
- NP_003248
- UniProt
- Q07157
- 途径
- Carbohydrate Homeostasis, Cell-Cell Junction Organization
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