Cytokeratin 7 抗体
Quick Overview for Cytokeratin 7 抗体 (ABIN6655254)
抗原
See all Cytokeratin 7 (KRT7) 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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Supplier Product No.
- 200-301-p34
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Supplier
- Rockland
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原理
- Cytokeratin Peptide 7 Antibody
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交叉反应 (详细)
- This product reacts with human.
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纯化方法
- Anti-Cytokeratin Peptide 7 antibody was purified from mouse ascites by protein A chromatography.
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免疫原
- Cytokeratin Peptide 7 antibody was produced in mice by repeated immunizations with cytoskeletal preparation of the RT4 human bladder carcinoma cell line.
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亚型
- IgG1
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应用备注
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ELISA_Dilution: 1:20,000
Immunohistochemistry_Dilution: 1:100-1:500
Western_Blot_Dilution: 1-2 μg/mL
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说明
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Anti-Cytokeratin Peptide 7 is tested for Immunohistochemistry -P, -F, and Western Blot. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately ~51 kDa corresponding to the appropriate cell lysate or extract.
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
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Reconstitution_Buffer: Neutral PBS
Reconstitution_Volume: 1.0 mL
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缓冲液
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Buffer: 1.2 % sodium acetate
Stabilizer: 2 mg BSA
Preservative: 0.01 % (w/v) Sodium Azide -
储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C,-20 °C
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储存方法
- Store vial at 4° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
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有效期
- 12 months
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- Cytokeratin 7 (KRT7) (Keratin 7 (KRT7))
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别名
- Cytokeratin Peptide 7
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背景
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Synonyms: SCL, cytokeratin 7, K2C7, K7, keratin, Keratin-7, 55K type II cytoskeletal, keratin, type II cytoskeletal 7, sarcolectin, , KERATIN, SIMPLE EPITHELIAL, KERATIN, TYPE II, CYTOSKELETAL, 7, SARCOLECTIN, Type-II keratin Kb7
Background: KRT7 is a type II keratin of simple nonkeratinizing epithelia. The deduced 489-amino acid protein has a calculated molecular mass of about 54 kD. K7 contains 4 central alpha-helical segments with heptad repeats of hydrophobic residues characteristic of a coiled-coil region. Within this domain, K7 shares 73 % homology with epidermal K6B. KRT7 gene contains 9 exons and spans more than 15.6 kb. K7 gene is mapped to chromosome 12. Keratin 7 is expressed in a wide range of epithelial structures in humans. This antibody is suitable for researchers interested in cancer research, cardiovascular diseases, and cytoskeletal signaling research.
Gene Name: KRT7
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基因ID
- 3855
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NCBI登录号
- NP_005547
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UniProt
- P08729
抗原
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