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- 抗原
- CD172a/b
- 适用
- 人
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宿主
- 小鼠
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克隆类型
- 单克隆
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标记
- 非结合性
- 应用范围
- Flow Cytometry (FACS)
- 特异性
- The mouse monoclonal antibody SE5A5 recognizes a common extracellular epitope on human CD172a and CD172b antigens (approx. 90 kDa and approx. 50 kDa, respectively), although its reactivity with CD172a is higher.
- 交叉反应 （详细）
- Human
- 纯化方法
- Purified by protein-A affinity chromatography.
- 纯度
- > 95 % (by SDS-PAGE)
- 免疫原
- NIH-3T3 / human CD172a cell line
- 克隆位点
- SE5A5
- 亚型
- IgG1
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anti-CD172a/b antibody (PE)
适用: 人 FACS 宿主: 小鼠 Monoclonal SE5A5 PE
anti-CD172a/b antibody (APC)适用: 人 FACS 宿主: 小鼠 Monoclonal SE5A5 APC
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- 应用备注
- Flow cytometry: Recommended dilution: 1-4 μg/mL.
- 限制
- 仅限研究用
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- 浓度
- 1 mg/mL
- 缓冲液
- Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C
- 储存方法
- Store at 2-8°C. Do not freeze.
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- 抗原
- CD172a/b
- 背景
- CD172a, the signal-regulatory protein alpha (SIRP alpha), also known as SH2 domain-containing phosphatase substrate-1 (SHPS1), is a 75-110 kDa transmembrane glycoprotein expressed mainly on granulocytes, monocytes, macrophages, dendritic cells and neurons. Its extracellular ligand is CD47. CD172a serves as a substrate of activated receptor tyrosine kinases and upon phosphorylation it recruits SH2 domain-containing tyrosine phosphatases, thereby regulating signal transduction processes related to cell activation, transmigration and phagocytosis. CD172a is a specific marker of cardiomyocytes derived from human pluripotent stem cells and serves as a negative regulator of signaling and growth in myeloid progenitor cells. Extracellular part of CD172b is 90 % identical to that of CD172a, but unlike CD172, it has dramatically reduced intracellular domain.,PTPNS1, BIT, MFR, SIRPA, SHPS1, SIRPB1,
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