ATP1A2 抗体 (AA 451-479)
(2 references)
(1 validation)-
- 抗原 See all ATP1A2 抗体
- ATP1A2 (ATPase, Na+/K+ Transporting, alpha 2 Polypeptide (ATP1A2))
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抗原表位
- AA 451-479
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适用
- 人, 小鼠
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宿主
- 兔
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克隆类型
- 多克隆
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标记
- This ATP1A2 antibody is un-conjugated
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应用范围
- Western Blotting (WB), Immunofluorescence (IF), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
- 预测反应
- B, Pig, Rat
- 纯化方法
- This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
- 免疫原
- This ATP1A2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 451-479 amino acids from the Central region of human ATP1A2.
- 克隆位点
- RB19033
- 亚型
- Ig Fraction
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anti-ATPase, Na+/K+ Transporting, alpha 2 Polypeptide (ATP1A2) (AA 7-19), (Intracellular), (N-Term) antibody
ATP1A2 适用: 人, 小鼠, 大鼠 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-ATPase, Na+/K+ Transporting, alpha 2 Polypeptide (ATP1A2) (N-Term) antibodyATP1A2 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-ATPase, Na+/K+ Transporting, alpha 2 Polypeptide (ATP1A2) (AA 1-70) antibodyATP1A2 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
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- 应用备注
- IF: 1:10~50. WB: 1:2000. WB: 1:1000. WB: 1:1000. IHC-P: 1:10~50. FC: 1:10~50
- 限制
- 仅限研究用
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- by
- Memorial Sloan-Kettering Cancer Center, Parada lab
- No.
- #100013
- 日期
- 2016.05.11
- 抗原
- ATPase, Na+/K+ Transporting, alpha 2 Polypeptide (ATP1A2)
- Lot Number
- SH09708BF
- Method validated
- Immunofluorescence
- Positive Control
- Wild-type mouse adult brain cryo-sections
- Negative Control
- Rabbit IgG
- Notes
- The antibody showed signals along the subventricular zone, which is consistent with the RNA expression analysis.
- Primary Antibody
- ABIN652011
- Secondary Antibody
- Donkey-anti-Rabbit-Cy3
- Full Protocol
- Wild-type mouse adult brain was dissected, fixed in 4% PFA for over/night, saturated in 30% sucrose, mounted in Cryo, and sectioned into 12µm sections.
- Immunostaining for Cryo Sections:
- Dry slides at 50-55°C for 15-30min (or RT for 30min).
- Wash with PBS 5min X 3 (shake gently).
- Block with 10% Normal Donkey Serum (NDS) at RT for 1h.
- Incubate with ABIN652011 diluted 1:10 - 1:800 in 2% NDS at 4°C overnight.
- Wash with PBS 5min X 3.
- Incubate with the secondary antibody Donkey-anti-Rabbit-Cy3 together with DAPI 1:100 at RT for 1h (prepared in 2% NDS), cover the chamber with foil.
- Wash with PBS 5min X 3.
- Add three drops of water-based Immu-mount.
- Cover with 24 X 60 cover glass.
- Imaging:
- Images were taken using a Zeiss Axio2-Imaging system, 20X-0.5NA-Dry Dye-1004-072 objective, DAPI and Cy3 channel.
- Experimental Notes
- ABIN652011 worked at 1:10, did not work well in 1:50-1:200.
生效 #100013 (Immunofluorescence)!['独立验证'标志]( "成功验证")
!['独立验证'标志]( "成功验证")
Validation Images![IF validation of ATP1A2 antibody ABIN652011 on mouse brain sections.
A. ABIN652011 diluted 1:10 (red), DAPI counterstaining (blue).
B. Dilution factor 1:10. C. Dilution factor 1:50. D. Dilution factor 1:200.]()
IF validation of ATP1A2 antibody ABIN652011 on mouse brain sections.
A. ABIN652011 diluted 1:10 (red), DAPI counterstaining (blue).
B. Dilution factor 1:10. C. Dilution factor 1:50. D. Dilution factor 1:200.
Full Methods -
- 状态
- Liquid
- 缓冲液
- Purified polyclonal antibody supplied in PBS with 0.09 % (W/V) sodium azide.
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C,-20 °C
- 储存方法
- Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.
- 有效期
- 6 months
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-
: "Genetic Deletion of TREK-1 or TWIK-1/TREK-1 Potassium Channels does not Alter the Basic Electrophysiological Properties of Mature Hippocampal Astrocytes In Situ." in: Frontiers in cellular neuroscience, Vol. 10, pp. 13, (2016) (PubMed).
: "mGluR3 Activation Recruits Cytoplasmic TWIK-1 Channels to Membrane that Enhances Ammonium Uptake in Hippocampal Astrocytes." in: Molecular neurobiology, (2015) (PubMed).
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: "Genetic Deletion of TREK-1 or TWIK-1/TREK-1 Potassium Channels does not Alter the Basic Electrophysiological Properties of Mature Hippocampal Astrocytes In Situ." in: Frontiers in cellular neuroscience, Vol. 10, pp. 13, (2016) (PubMed).
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- 抗原
- ATP1A2 (ATPase, Na+/K+ Transporting, alpha 2 Polypeptide (ATP1A2))
- 别名
- ATP1A2 (ATP1A2 产品)
- 别名
- C11orf29 antibody, TIF1D antibody, TIF1DELTA antibody, AW060654 antibody, Atpa-3 antibody, mKIAA0778 antibody, RATATPA2 antibody, FHM2 antibody, MHP2 antibody, atp1a2 antibody, atp[a]2 antibody, atpa2 antibody, cb702 antibody, fa10d04 antibody, fa10d05 antibody, wu:fa10d05 antibody, ATP2A antibody, atp1a2.L antibody, fhm2 antibody, mhp2 antibody, ATP1A2 antibody, ATP1A4 antibody, tripartite motif containing 66 antibody, ATPase, Na+/K+ transporting, alpha 2 polypeptide antibody, ATPase Na+/K+ transporting subunit alpha 2 antibody, ATPase, Na+/K+ transporting, alpha 2a polypeptide antibody, sodium/potassium-transporting ATPase subunit alpha-2 antibody, ATPase, Na+/K+ transporting, alpha 2 polypeptide S homeolog antibody, TRIM66 antibody, Atp1a2 antibody, ATP1A2 antibody, atp1a2a antibody, atp1a2.S antibody, LOC488636 antibody
- 分子量
- 112265
- 基因ID
- 477
- NCBI登录号
- NP_000693
- UniProt
- P50993
- 途径
- Thyroid Hormone Synthesis, Proton Transport, Ribonucleoside Biosynthetic Process
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