MTOR 抗体
(7 references)-
- 抗原 See all MTOR (mTOR) 抗体
- MTOR (mTOR) (Mechanistic Target of Rapamycin (serine/threonine Kinase) (mTOR))
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适用
- 人, 小鼠, 大鼠, 鱼
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宿主
- 兔
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克隆类型
- 多克隆
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标记
- This MTOR antibody is un-conjugated
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应用范围
- Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF), Immunocytochemistry (ICC)
- 特异性
- MTOR Antibody detects endogenous levels of total mTOR
- 交叉反应
- 鱼, 人, 小鼠, 大鼠
- 预测反应
- Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(100%)
- 纯化方法
- The antiserum was purified by peptide affinity chromatography using SulfoLinkTM Coupling Resin (Thermo Fisher Scientific).
- 免疫原
- A synthesized peptide derived from human mTOR
- 亚型
- IgG
- Top Product
- Discover our top product mTOR Primary Antibody
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anti-Mechanistic Target of Rapamycin (serine/threonine Kinase) (mTOR) (AA 2436-2492) antibody
mTOR 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC (p), FACS, IF (cc), IF (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Mechanistic Target of Rapamycin (serine/threonine Kinase) (mTOR) (AA 2388-2487) antibodymTOR 适用: 人 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Mechanistic Target of Rapamycin (serine/threonine Kinase) (mTOR) (C-Term) antibodymTOR 适用: 人, 小鼠, 大鼠 WB, IF, IHC (p), IP, ICC, ChIP 宿主: 兔 Polyclonal unconjugated
anti-Mechanistic Target of Rapamycin (serine/threonine Kinase) (mTOR) (AA 2226-2488) antibodymTOR 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Mechanistic Target of Rapamycin (serine/threonine Kinase) (mTOR) (AA 2400-2500) antibodymTOR 适用: 人 WB, IF 宿主: 兔 Polyclonal unconjugated
anti-Mechanistic Target of Rapamycin (serine/threonine Kinase) (mTOR) (AA 2311-2529) antibodymTOR 适用: 人 WB, IHC, ELISA, FACS, ICC 宿主: 小鼠 Monoclonal 8H5A5 unconjugated
anti-Mechanistic Target of Rapamycin (serine/threonine Kinase) (mTOR) (AA 2459-2488) antibodymTOR 适用: 人 WB, IF, IHC (p) 宿主: 兔 Polyclonal RB13347 unconjugated
anti-Mechanistic Target of Rapamycin (serine/threonine Kinase) (mTOR) (AA 2459-2488) antibodymTOR 适用: 人 WB, IHC, ELISA, IF 宿主: 兔 Polyclonal unconjugated
anti-Mechanistic Target of Rapamycin (serine/threonine Kinase) (mTOR) (pSer2448) antibodymTOR 适用: 人, 小鼠, 大鼠, Cow, 鱼 WB, ELISA, IHC (p), FACS, IF (cc), IF (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Mechanistic Target of Rapamycin (serine/threonine Kinase) (mTOR) (pSer2481) antibodymTOR 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC (p), FACS, IF (cc), IF (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
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- 应用备注
- WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
- 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- 1 mg/mL
- 缓冲液
- Rabbit IgG in phosphate buffered saline , pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- -20 °C
- 储存方法
- Store at -20 °C.Stable for 12 months from date of receipt
- 有效期
- 12 months
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: "AMPK activation-dependent autophagy compromises oleanolic acid-induced cytotoxicity in human bladder cancer cells." in: Oncotarget, Vol. 8, Issue 40, pp. 67942-67954, (2018) (PubMed).
: "Omeprazole promotes carcinogenesis of fore-stomach in mice with co-stimulation of nitrosamine." in: Oncotarget, Vol. 8, Issue 41, pp. 70332-70344, (2018) (PubMed).
: "Respiratory Syncytial Virus Replication Is Promoted by Autophagy-Mediated Inhibition of Apoptosis." in: Journal of virology, Vol. 92, Issue 8, (2018) (PubMed).
: "Biological functions of lung cancer cells are suppressed in co-culture with mesenchymal stem cells isolated from umbilical cord." in: Experimental and therapeutic medicine, Vol. 15, Issue 1, pp. 1076-1080, (2018) (PubMed).
: "Selenium deficiency impaired immune function of the immune organs in young grass carp (Ctenopharyngodon idella)." in: Fish & shellfish immunology, Vol. 77, pp. 53-70, (2018) (PubMed).
: "Long non‑coding RNA SENCR alleviates the inhibitory effects of rapamycin on human umbilical vein endothelial cells." in: Molecular medicine reports, Vol. 18, Issue 2, pp. 1405-1414, (2018) (PubMed).
: "Quercetin induces autophagy via FOXO1-dependent pathways and autophagy suppression enhances quercetin-induced apoptosis in PASMCs in hypoxia." in: Free radical biology & medicine, Vol. 103, pp. 165-176, (2017) (PubMed).
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: "AMPK activation-dependent autophagy compromises oleanolic acid-induced cytotoxicity in human bladder cancer cells." in: Oncotarget, Vol. 8, Issue 40, pp. 67942-67954, (2018) (PubMed).
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- 抗原
- MTOR (mTOR) (Mechanistic Target of Rapamycin (serine/threonine Kinase) (mTOR))
- 别名
- mTOR (mTOR 产品)
- 别名
- FRAP1 antibody, FRAP antibody, FRAP2 antibody, RAFT1 antibody, RAPT1 antibody, 2610315D21Rik antibody, AI327068 antibody, Frap1 antibody, flat antibody, frap1 antibody, tor antibody, wu:fc22h08 antibody, mechanistic target of rapamycin kinase antibody, mechanistic target of rapamycin antibody, mechanistic target of rapamycin (serine/threonine kinase) antibody, MTOR antibody, Mtor antibody, mtor antibody
- 背景
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Description: Serine/threonine protein kinase which is a central regulator of cellular metabolism, growth and survival in response to hormones, growth factors, nutrients, energy and stress signals. MTOR directly or indirectly regulates the phosphorylation of at least 800 proteins. Functions as part of 2 structurally and functionally distinct signaling complexes mTORC1 and mTORC2 (mTOR complex 1 and 2). Activated mTORC1 up-regulates protein synthesis by phosphorylating key regulators of mRNA translation and ribosome synthesis. This includes phosphorylation of EIF4EBP1 and release of its inhibition toward the elongation initiation factor 4E (eiF4E). Moreover, phosphorylates and activates RPS6KB1 and RPS6KB2 that promote protein synthesis by modulating the activity of their downstream targets including ribosomal protein S6, eukaryotic translation initiation factor EIF4B, and the inhibitor of translation initiation PDCD4. Stimulates the pyrimidine biosynthesis pathway, both by acute regulation through RPS6KB1-mediated phosphorylation of the biosynthetic enzyme CAD, and delayed regulation, through transcriptional enhancement of the pentose phosphate pathway which produces 5-phosphoribosyl-1-pyrophosphate (PRPP), an allosteric activator of CAD at a later step in synthesis, this function is dependent on the mTORC1 complex. Regulates ribosome synthesis by activating RNA polymerase III-dependent transcription through phosphorylation and inhibition of MAF1 an RNA polymerase III-repressor. In parallel to protein synthesis, also regulates lipid synthesis through SREBF1/SREBP1 and LPIN1. To maintain energy homeostasis mTORC1 may also regulate mitochondrial biogenesis through regulation of PPARGC1A. mTORC1 also negatively regulates autophagy through phosphorylation of ULK1. Under nutrient sufficiency, phosphorylates ULK1 at 'Ser-758', disrupting the interaction with AMPK and preventing activation of ULK1. Also prevents autophagy through phosphorylation of the autophagy inhibitor DAP. mTORC1 exerts a feedback control on upstream growth factor signaling that includes phosphorylation and activation of GRB10 a INSR-dependent signaling suppressor. Among other potential targets mTORC1 may phosphorylate CLIP1 and regulate microtubules. As part of the mTORC2 complex MTOR may regulate other cellular processes including survival and organization of the cytoskeleton. Plays a critical role in the phosphorylation at 'Ser-473' of AKT1, a pro-survival effector of phosphoinositide 3-kinase, facilitating its activation by PDK1. mTORC2 may regulate the actin cytoskeleton, through phosphorylation of PRKCA, PXN and activation of the Rho-type guanine nucleotide exchange factors RHOA and RAC1A or RAC1B. mTORC2 also regulates the phosphorylation of SGK1 at 'Ser-422' (PubMed:12087098, PubMed:12150925, PubMed:12150926, PubMed:12231510, PubMed:12718876, PubMed:14651849, PubMed:15268862, PubMed:15467718, PubMed:15545625, PubMed:15718470, PubMed:18497260, PubMed:18762023, PubMed:18925875, PubMed:20516213, PubMed:20537536, PubMed:21659604, PubMed:23429703, PubMed:23429704, PubMed:25799227, PubMed:26018084). Regulates osteoclastogenesis by adjusting the expression of CEBPB isoforms (By similarity).
Gene: MTOR
- 分子量
- 289kDa
- 基因ID
- 2475
- UniProt
- P42345
- 途径
- PI3K-Akt Signaling, RTK signaling, AMPK Signaling, Interferon-gamma Pathway, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Regulation of Actin Filament Polymerization, Regulation of Muscle Cell Differentiation, Regulation of Cell Size, Skeletal Muscle Fiber Development, Regulation of Carbohydrate Metabolic Process, Autophagy, CXCR4-mediated Signaling Events, BCR Signaling, Warburg Effect
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