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- 抗原 See all HLA-DRB3 抗体
- HLA-DRB3 (Major Histocompatibility Complex, Class II, DR beta 3 (HLA-DRB3))
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适用
- 人, 大鼠, 小鼠
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宿主
- 兔
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克隆类型
- 多克隆
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标记
- This HLA-DRB3 antibody is un-conjugated
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应用范围
- Western Blotting (WB), ELISA, Immunohistochemistry (IHC)
- 特异性
- HLA-DRB3 Antibody detects endogenous levels of total HLA-DRB3
- 交叉反应
- 人, 小鼠, 大鼠
- 纯化方法
- The antiserum was purified by peptide affinity chromatography using SulfoLinkTM Coupling Resin (Thermo Fisher Scientific).
- 免疫原
- A synthesized peptide derived from human HLA-DRB3
- 亚型
- IgG
- Top Product
- Discover our top product HLA-DRB3 Primary Antibody
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anti-Major Histocompatibility Complex, Class II, DR beta 3 (HLA-DRB3) (AA 1-266) antibody
HLA-DRB3 适用: 人 WB, FACS 宿主: 小鼠 Polyclonal unconjugated
anti-Major Histocompatibility Complex, Class II, DR beta 3 (HLA-DRB3) antibodyHLA-DRB3 适用: 人 IHC 宿主: 兔 Polyclonal unconjugated
anti-Major Histocompatibility Complex, Class II, DR beta 3 (HLA-DRB3) (AA 111-227) antibodyHLA-DRB3 适用: 人 WB, ELISA, IF 宿主: 兔 Polyclonal unconjugated
anti-Major Histocompatibility Complex, Class II, DR beta 3 (HLA-DRB3) (AA 1-266) antibodyHLA-DRB3 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
anti-Major Histocompatibility Complex, Class II, DR beta 3 (HLA-DRB3) antibodyHLA-DRB3 适用: 人 ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-Major Histocompatibility Complex, Class II, DR beta 3 (HLA-DRB3) (AA 150-199) antibodyHLA-DRB3 适用: 人, 猴 WB 宿主: 兔 Polyclonal unconjugated
anti-Major Histocompatibility Complex, Class II, DR beta 3 (HLA-DRB3) (AA 30-227) antibodyHLA-DRB3 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
anti-Major Histocompatibility Complex, Class II, DR beta 3 (HLA-DRB3) antibodyHLA-DRB3 适用: 人 IF, ICC 宿主: 兔 Polyclonal unconjugated
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- 应用备注
- WB 1:500-1:2000 IHC 1:50-1:200
- 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- 1 mg/mL
- 缓冲液
- Rabbit IgG in phosphate buffered saline , pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- -20 °C
- 储存方法
- Store at -20 °C.Stable for 12 months from date of receipt
- 有效期
- 12 months
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- 抗原
- HLA-DRB3 (Major Histocompatibility Complex, Class II, DR beta 3 (HLA-DRB3))
- 别名
- HLA-DRB3 (HLA-DRB3 产品)
- 别名
- HLA-DR3B antibody, BLA-DRB3 antibody, Bota-DRB01 antibody, Bota-DRB02 antibody, Bota-DRB04 antibody, Bota-DRB07 antibody, Bota-DRB21 antibody, Bota-DRB22 antibody, LA-DRB antibody, major histocompatibility complex, class II, DR beta 3 antibody, major histocompatibility complex, class II, DRB3 antibody, HLA-DRB3 antibody, BOLA-DRB3 antibody
- 背景
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Description: Binds peptides derived from antigens that access the endocytic route of antigen presenting cells (APC) and presents them on the cell surface for recognition by the CD4 T-cells. The peptide binding cleft accommodates peptides of 10-30 residues. The peptides presented by MHC class II molecules are generated mostly by degradation of proteins that access the endocytic route, where they are processed by lysosomal proteases and other hydrolases. Exogenous antigens that have been endocytosed by the APC are thus readily available for presentation via MHC II molecules, and for this reason this antigen presentation pathway is usually referred to as exogenous. As membrane proteins on their way to degradation in lysosomes as part of their normal turn-over are also contained in the endosomal/lysosomal compartments, exogenous antigens must compete with those derived from endogenous components. Autophagy is also a source of endogenous peptides, autophagosomes constitutively fuse with MHC class II loading compartments. In addition to APCs, other cells of the gastrointestinal tract, such as epithelial cells, express MHC class II molecules and CD74 and act as APCs, which is an unusual trait of the GI tract. To produce a MHC class II molecule that presents an antigen, three MHC class II molecules (heterodimers of an alpha and a beta chain) associate with a CD74 trimer in the ER to form a heterononamer. Soon after the entry of this complex into the endosomal/lysosomal system where antigen processing occurs, CD74 undergoes a sequential degradation by various proteases, including CTSS and CTSL, leaving a small fragment termed CLIP (class-II-associated invariant chain peptide). The removal of CLIP is facilitated by HLA-DM via direct binding to the alpha-beta-CLIP complex so that CLIP is released. HLA-DM stabilizes MHC class II molecules until primary high affinity antigenic peptides are bound. The MHC II molecule bound to a peptide is then transported to the cell membrane surface. In B-cells, the interaction between HLA-DM and MHC class II molecules is regulated by HLA-DO. Primary dendritic cells (DCs) also to express HLA-DO. Lysosomal microenvironment has been implicated in the regulation of antigen loading into MHC II molecules, increased acidification produces increased proteolysis and efficient peptide loading.
Gene: HLA-DRB3
- 分子量
- 30kDa
- 基因ID
- 3125
- UniProt
- P79483
- 途径
- TCR Signaling, Positive Regulation of Peptide Hormone Secretion, Production of Molecular Mediator of Immune Response, Cancer Immune Checkpoints
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