RFWD2 抗体 (Internal Region)
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- 抗原 See all RFWD2 抗体
- RFWD2 (Ring Finger and WD Repeat Domain 2, E3 Ubiquitin Protein Ligase (RFWD2))
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抗原表位
- Internal Region
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适用
- 人, 小鼠, 大鼠
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宿主
- 兔
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克隆类型
- 多克隆
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标记
- This RFWD2 antibody is un-conjugated
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应用范围
- Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC)
- 特异性
- RFWD2 Antibody detects endogenous levels of total RFWD2.
- 预测反应
- Bovine,Chicken
- 纯化方法
- The antiserum was purified by peptide affinity chromatography using SulfoLinkTM Coupling Resin (Thermo Fisher Scientific).
- 免疫原
- A synthesized peptide derived from human RFWD2, corresponding to a region within the internal amino acids.
- 亚型
- IgG
- Top Product
- Discover our top product RFWD2 Primary Antibody
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anti-Ring Finger and WD Repeat Domain 2, E3 Ubiquitin Protein Ligase (RFWD2) (AA 632-731) antibody
RFWD2 适用: 人 WB, ELISA, IF 宿主: 小鼠 Monoclonal 1E4 unconjugated
anti-Ring Finger and WD Repeat Domain 2, E3 Ubiquitin Protein Ligase (RFWD2) antibodyRFWD2 适用: 人 WB, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Ring Finger and WD Repeat Domain 2, E3 Ubiquitin Protein Ligase (RFWD2) (Tyr762) antibodyRFWD2 适用: 人, 小鼠 WB, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Ring Finger and WD Repeat Domain 2, E3 Ubiquitin Protein Ligase (RFWD2) (AA 301-350) antibodyRFWD2 适用: 人, 小鼠, 兔, Cow, 马, 犬, 豚鼠, Pig, Bat, 猴 WB 宿主: 兔 Polyclonal unconjugated
anti-Ring Finger and WD Repeat Domain 2, E3 Ubiquitin Protein Ligase (RFWD2) (AA 582-731) antibodyRFWD2 适用: 人 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Ring Finger and WD Repeat Domain 2, E3 Ubiquitin Protein Ligase (RFWD2) (AA 37-65), (N-Term) antibodyRFWD2 适用: 人 WB, FACS 宿主: 兔 Polyclonal RB19572 unconjugated
anti-Ring Finger and WD Repeat Domain 2, E3 Ubiquitin Protein Ligase (RFWD2) (AA 672-701), (C-Term) antibodyRFWD2 适用: 人 WB 宿主: 兔 Polyclonal RB41831 unconjugated
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- 应用备注
- WB 1:500-1:1000, IF/ICC 1:100-1:500, IHC 1:50-1:200, ELISA(peptide) 1:20000-1:40000
- 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- 1 mg/mL
- 缓冲液
- Rabbit IgG in phosphate buffered saline , pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- -20 °C
- 储存方法
- Store at -20 °C. Stable for 12 months from date of receipt.
- 有效期
- 12 months
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- 抗原
- RFWD2 (Ring Finger and WD Repeat Domain 2, E3 Ubiquitin Protein Ligase (RFWD2))
- 别名
- RFWD2 (RFWD2 产品)
- 别名
- COP1 antibody, RNF200 antibody, AI316802 antibody, C80879 antibody, Cop1 antibody, RGD1304773 antibody, RFWD2 antibody, cop1 antibody, rnf200 antibody, zgc:163067 antibody, ring finger and WD repeat domain 2 antibody, ring finger and WD repeat domain 2, E3 ubiquitin protein ligase antibody, ring finger and WD repeat domain 2, E3 ubiquitin protein ligase L homeolog antibody, RFWD2 antibody, Rfwd2 antibody, rfwd2 antibody, rfwd2.L antibody
- 背景
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Description: E3 ubiquitin-protein ligase that mediates ubiquitination and subsequent proteasomal degradation of target proteins. E3 ubiquitin ligases accept ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. Involved in JUN ubiquitination and degradation. Directly involved in p53 (TP53) ubiquitination and degradation, thereby abolishing p53-dependent transcription and apoptosis. Ubiquitinates p53 independently of MDM2 or RCHY1. Probably mediates E3 ubiquitin ligase activity by functioning as the essential RING domain subunit of larger E3 complexes. In contrast, it does not constitute the catalytic RING subunit in the DCX DET1-COP1 complex that negatively regulates JUN, the ubiquitin ligase activity being mediated by RBX1. Involved in 14-3-3 protein sigma/SFN ubiquitination and proteasomal degradation, leading to AKT activation and promotion of cell survival. Ubiquitinates MTA1 leading to its proteasomal degradation. Upon binding to TRIB1, ubiquitinates CEBPA, which lacks a canonical COP1-binding motif (Probable).
Gene: RFWD2
- 分子量
- 80 kDa
- 基因ID
- 64326
- UniProt
- Q8NHY2
- 途径
- Photoperiodism
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