小鼠 anti-人 kappa Light Chain Antibody
FACS, WB, IHC (f), IF
Monoclonal
L1C1
IgG1 kappa
unconjugated
2 images
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- 抗原 See all kappa Light Chain products
- kappa Light Chain
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适用
- 人
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宿主
- 小鼠
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克隆类型
- 单克隆
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应用范围
- Flow Cytometry (FACS), Western Blotting (WB), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)), Immunofluorescence (IF)
- 原理
- Mouse Monoclonal anti-Human Kappa Light Chain (L1C1), Purified, BSA-free
- 产品特性
- This MAb is specific to kappa light chain of immunoglobulin and shows no cross-reaction with lambda light chain or any of the five heavy chains. In mammals, the two light chains in an antibody are always identical, with only one type of light chain, kappa or lambda. The ratio of kappa to lambda is 70:30. However, with the occurrence of multiple myeloma or other B-cell malignancies this ratio is disturbed. Antibody to the kappa light chain is reportedly useful in the identification of leukemias, plasmacytomas, and certain non-Hodgkin's lymphomas. Demonstration of clonality in lymphoid infiltrates indicates that the infiltrate is malignant. Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.
- 纯化方法
- Purified
- 免疫原
- Human B-Lymphoma Cells
- 克隆位点
- L1C1
- 亚型
- IgG1 kappa
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- 应用备注
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Does not react with rat, others not known
- Immunohistology formalin-fixed 0.5-1 μg/mL
- Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes
- Immunofluorescence 0.5-1 μg/mL
- Western blotting 0.5-1 μg/mL
- Flow Cytometry 0.5-2.0 μg/million cells in 0.1 mL
- Optimal dilution for a specific application should be determined by user
- 说明
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293T, Raji or hPBL cells. Tonsil or Spleen
- 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- 1 mg/mL
- 缓冲液
- PBS (no BSA, no azide)
- 储存液
- Azide free
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- 抗原
- kappa Light Chain
- Abstract
- kappa Light Chain 产品
- 背景
- HCAK1, Ig Kappa Chain C Region, IGKC, Immunoglobulin KM
- 分子量
- ~22.5 kDa
- 基因ID
- 3514, 449609
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