CEACAM5 抗体
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- 抗原 See all CEACAM5 抗体
- CEACAM5 (Carcinoembryonic Antigen-Related Cell Adhesion Molecule 5 (CEACAM5))
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适用
- 人
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宿主
- 小鼠
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克隆类型
- 单克隆
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标记
- This CEACAM5 antibody is un-conjugated
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应用范围
- Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)), Western Blotting (WB)
- 原理
- Mouse Monoclonal anti-CD66 / CEA (COL-1), Purified, BSA-free
- 产品特性
- This antibody recognizes proteins of 80-200 kDa, identified as different members of CEA family. CEA is synthesized during development in the fetal gut and is re-expressed in increased amounts in intestinal carcinomas and several other tumors. This MAb does not react with nonspecific cross-reacting antigen (NCA) and with human polymorphonuclear leucocytes. It shows no reaction with a variety of normal tissues and is suitable for staining of formalin/paraffin tissues. CEA is not found in benign glands, stroma, or malignant prostatic cells. Antibody to CEA is useful in detecting early foci of gastric carcinoma and in distinguishing pulmonary adenocarcinomas (60-70 % are CEA ) from pleural mesotheliomas (rarely or weakly CEA ). Anti-CEA positivity is seen in adenocarcinomas from the lung, colon, stomach, esophagus, pancreas, gallbadder, urachus, salivary gland, ovary, and endocervix. Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.
- 纯化方法
- Purified
- 免疫原
- Human colon carcinoma extract
- 克隆位点
- COL-1
- 亚型
- IgG2a kappa
- Top Product
- Discover our top product CEACAM5 Primary Antibody
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- 应用备注
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Immunohistology formalin-fixed 0.5-1 μg/mL
- Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes
- Flow Cytometry 0.5-1 μg/million cells/0.1 mL
- Immunofluorescence 1-2 μg/mL
- Western blotting 0.5-1 μg/mL
- Optimal dilution for a specific application should be determined by user
- 说明
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MCF7 or 293T cells. Colon carcinoma
- 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- 1 mg/mL
- 缓冲液
- PBS (no BSA, no azide)
- 储存液
- Azide free
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- 抗原
- CEACAM5 (Carcinoembryonic Antigen-Related Cell Adhesion Molecule 5 (CEACAM5))
- 别名
- CD66 (CEA) (CEACAM5 产品)
- 别名
- CD66e antibody, CEA antibody, CEACAM5 antibody, CEACAM antibody, 1600029H12Rik antibody, Psg30 antibody, carcinoembryonic antigen related cell adhesion molecule 5 antibody, carcinoembryonic antigen antibody, carcinoembryonic antigen-related cell adhesion molecule 5 antibody, carcinoembryonic antigen-related cell adhesion molecule 8 antibody, carcinoembryonic antigen gene family antibody, CEACAM5 antibody, Ceacam5 antibody, LOC100409241 antibody, Cea antibody
- 背景
- Carcinoembryonic Antigen-related Cell Adhesion Molecule 5, CEACAM5, CD66, Biliary Glycoprotein (BGP-1)
- 分子量
- 80-200 kDa
- 基因ID
- 1048, 634, 709196
- UniProt
- P06731
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