(3 references)-
- 抗原 See all IgG products
- IgG
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抗原表位
- Heavy & Light Chain
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适用
- 山羊
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宿主
- 驴
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标记
- DyLight 594
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应用范围
- Flow Cytometry (FACS), Immunofluorescence (IF)
- 产品特性
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Donkey anti-Goat IgG (H&L) - Affinity Pure, DyLight 594 Conjugate.
Fluorphore: DyLight 594 (Ex = 593 nm, Em = 618 nm).
Fluor Protein Ratio: Moles DyLight 594 per Mole Antibody. - 纯化方法
- Affinity purified using solid phase mouse IgG (H&L)
- 纯度
- > 95 % based on SDS-PAGE
- 免疫原
- Purified Goat IgG, whole molecule
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Chicken anti-Goat IgG antibody (DyLight 550)
WB, IF Polyclonal IgY DyLight 550
Donkey anti-Goat IgG (Heavy & Light Chain) antibody (Texas Red (TR)) - PreadsorbedFACS, FLISA, FM Polyclonal IgG Texas Red (TR)
Donkey anti-Donkey IgG (Heavy & Light Chain) antibody (PE) - PreadsorbedFACS, FM Polyclonal IgG PE
Donkey anti-Goat IgG (Heavy & Light Chain) antibody (PE) - PreadsorbedWB, FACS, FLISA, FM Polyclonal IgG PE F(ab')2 fragment
Chicken anti-Goat IgG antibody (DyLight 488)WB, IF Polyclonal IgY DyLight 488
Rabbit anti-Goat IgG (Heavy & Light Chain) antibody (Biotin)ELISA, IHC, WB, FACS Polyclonal IgG Biotin
Pig anti-Goat IgG (Heavy & Light Chain) antibodyELISA, IHC, WB, FACS Polyclonal IgG unconjugated
Donkey anti-Goat IgG (Heavy & Light Chain) antibodyELISA, IHC, WB, FACS Polyclonal IgG unconjugated
Pig anti-Goat IgG (Heavy & Light Chain) antibody (Biotin)ELISA, IHC, WB, FACS Polyclonal IgG Biotin
Pig anti-Goat IgG (Heavy & Light Chain) antibody (FITC)ELISA, IHC, FACS Polyclonal IgG FITC
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- 应用备注
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This conjugate is suitable for immunomicroscopy, flow cytometry.
The optimal working dilution should be determined by the investigator. Suggested starting dilution: 1:20 - 1:2,000 for most applications - 说明
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Country of Origin: Donkey serum was obtained from healthy animals of US origin, under the care of a registered veterinarian.
DyLight is a trademark of Thermo Fisher Scientific, Inc. and its subsidiaries. - 限制
- 仅限研究用
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- 状态
- Lyophilized
- 浓度
- 1 mg/mL
- 缓冲液
- 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 1 % (w/v) BSA, Protease/IgG free. 0.05 % (w/v) Sodium Azide
- 储存液
- Sodium azide
- 注意事项
- WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
- 注意事项
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Product is photosensitive and should be protected from light.
Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming.
A solution with 50 % glycerol will not freeze in -20 °C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. - 储存条件
- 4 °C
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: "v-Src induces tyrosine phosphorylation of focal adhesion kinase independently of tyrosine 397 and formation of a complex with Src." in: The Journal of biological chemistry, Vol. 275, Issue 30, pp. 23333-9, (2000) (PubMed).
: "Phosphospecific antibodies reveal focal adhesion kinase activation loop phosphorylation in nascent and mature focal adhesions and requirement for the autophosphorylation site." in: Cell growth & differentiation : the molecular biology journal of the American Association for Cancer Research, Vol. 11, Issue 1, pp. 41-8, (2000) (PubMed).
: "Focal adhesion kinase tyrosine-861 is a major site of phosphorylation by Src." in: Biochemical and biophysical research communications, Vol. 228, Issue 3, pp. 662-8, (1997) (PubMed).
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: "v-Src induces tyrosine phosphorylation of focal adhesion kinase independently of tyrosine 397 and formation of a complex with Src." in: The Journal of biological chemistry, Vol. 275, Issue 30, pp. 23333-9, (2000) (PubMed).
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- 抗原
- IgG
- Abstract
- IgG 产品
- 物质类
- Antibody
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