MAML3
适用: 人
IF (cc), IF (p)
宿主: 兔
Polyclonal
Alexa Fluor 750
应用备注
The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the MAML3 antibody to be titered up or down for optimal performance.\. Prediluted IHC only format : incubate for 30 min at RT
限制
仅限研究用
缓冲液
Prediluted in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05 % sodium azide, *For IHC use only*
储存液
Sodium azide
注意事项
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
储存条件
4 °C,-20 °C
储存方法
Store the MAML3 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
Mastermind-like 3 is a nuclear speckle protein that acts as a transcriptional coactivator for Notch receptors. The Notch signaling pathway influences cell fate by regulating the ability of precursor cells to properly respond to developmental signals. MAML3 is a member of the mastermind-like family of proteins that are human homologs of the Drosophila melanogaster mastermind protein. Through its N-terminal region, MAML3 interacts with the ankyrin repeats of the Notch 1-4 proteins. This interaction leads to formation of a DNA-binding complex with the Notch proteins and RBP-Jκ, a complex that can then induce HES1 gene expression. While the N-terminal domain of MAML3 is essential for proper Notch binding, the C-terminal domain is essential for transcriptional activation. Due to its involvement in cell signaling and transcriptional activation, its upregulation is thought to be involved in oncogenesis.