兔 anti-猴 IgG (Heavy & Light Chain) Antibody (FITC) - Preadsorbed

Synonyms: rabbit Anti-Monkey IgG peroxidase Conjugated Antibody, rabbit Anti-Monkey IgG Antibody peroxidase Conjugation, rabbit Anti-Monkey IgG HRP Conjugated Antibody

Background: Anti-Monkey IgG (H&L) FITC antibody generated in rabbit detects specifically monkey IgG heavy and light chains. Secreted as part of the adaptive immune response by plasma B cells, immunoglobulin G constitutes 75 % of serum immunoglobulins. IgG binds to viruses, bacteria, as well as fungi and facilitates their destruction or neutralization via agglutination (and thereby immobilizing them), activation of the compliment cascade, and opsinization for phagocytosis. The whole IgG molecule possesses both the F(c) region, recognized by high-affinity Fc receptor proteins, as well as the F(ab) region possessing the epitope-recognition site. Both heavy and light chains of the antibody molecule are present. This fluorescence conjugated anti-Monkey IgG (H&L) secondary antibody is ideal for investigators who routinely perform immunofluorescence, flow cytometry, and more general immunoassays. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment.

Immunogen: Anti-Monkey IgG (H&L) was produced by repeated immunization with monkey IgG in rabbit.

Immunogen Type: Native Protein

Application Note: Anti-Monkey IgG (H&L) FITC conjugated Antibody is suitable for use in immunoelectrophoresis, immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. Specific conditions for reactivity and signal detection should be optimized by the end user.

FLISA Dilution: 1:50,000 - 1:100,000

Flow Cytometry Dilution: User Optimized

Western Blot Dilution: 1:10,000 - 1:60,000

IF Microscopy Dilution: 1:1,000 - 1:5,000

Reconstitution Volume: 1.0 mL

Reconstitution Buffer: Restore with deionized water (or equivalent)

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Preservative: 0.01 % (w/v) Sodium Azide

Synonyms: rabbit Anti-Monkey IgG peroxidase Conjugated Antibody, rabbit Anti-Monkey IgG Antibody peroxidase Conjugation, rabbit Anti-Monkey IgG HRP Conjugated Antibody

Background: Anti-Monkey IgG (H&L) FITC antibody generated in rabbit detects specifically monkey IgG heavy and light chains. Secreted as part of the adaptive immune response by plasma B cells, immunoglobulin G constitutes 75 % of serum immunoglobulins. IgG binds to viruses, bacteria, as well as fungi and facilitates their destruction or neutralization via agglutination (and thereby immobilizing them), activation of the compliment cascade, and opsinization for phagocytosis. The whole IgG molecule possesses both the F(c) region, recognized by high-affinity Fc receptor proteins, as well as the F(ab) region possessing the epitope-recognition site. Both heavy and light chains of the antibody molecule are present. This fluorescence conjugated anti-Monkey IgG (H&L) secondary antibody is ideal for investigators who routinely perform immunofluorescence, flow cytometry, and more general immunoassays. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment.