山羊 anti-雪貂 IgG (Heavy Chain) Antibody (HRP) - Preadsorbed
-
- 抗原 See all IgG products
- IgG
-
抗原表位
- Heavy Chain
-
适用
- 雪貂
-
宿主
- 山羊
-
克隆类型
- 多克隆
-
标记
- HRP
-
应用范围
- ELISA, Immunohistochemistry (IHC), Western Blotting (WB)
- 交叉反应
- 雪貂
- 产品特性
- Anti-Ferret IgG (gamma chain) peroxidase conjugated antibody generated in goat detects specifically ferret IgG (gamma chain). This secondary peroxidase conjugated antibody anti-Ferret is ideal for investigators who routinely perform titration assays, western-blot, immunoprecipitation and more generally immunoassays.
- 纯化方法
- Preadsorption: Solid phase absorption
- 免疫原
-
Immunogen: Anti-Ferret IgG (gamma chain) was produced by repeated immunization with ferret IgG gamma heavy chain in goat.
Immunogen Type: Native Protein
- 亚型
- IgG
-
-
- 应用备注
-
Immunohistochemistry Dilution: 1:500 - 1:2,500
Application Note: Antibody Anti-Ferret IgG (gamma chain) peroxidase conjugated is suitable for immunoblotting (western or dot blot), ELISA, immunoperoxidase electron microscopy and immunohistochemistry as well as other peroxidase-antibody based enzymatic assays requiring lot-to-lot consistency.
ELISA Dilution: 1:10,000 - 1:50,000
Western Blot Dilution: 1:1,000 - 1:5,000
- 限制
- 仅限研究用
-
- 状态
- Lyophilized
- 溶解方式
-
Reconstitution Volume: 1.0 mL
Reconstitution Buffer: Restore with deionized water (or equivalent)
- 浓度
- 1.0 mg/mL
- 缓冲液
-
Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Preservative: 0.01 % (w/v) Thimerosal
- 储存液
- Thimerosal (Merthiolate)
- 注意事项
- This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- RT,4 °C,-20 °C
- 储存方法
- Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
- 有效期
- 12 months
-
- 抗原
- IgG
- Abstract
- IgG 产品
- 物质类
- Antibody
- 背景
-
Synonyms: goat anti-Ferret IgG (gamma chain) Antibody peroxidase Conjugation, goat anti-Ferret IgG gamma HRP Conjugated Antibody
Background: Anti-Ferret IgG Peroxidase Antibody generated in goat detects ferret IgG. Secreted as part of the adaptive immune response by plasma B cells, immunoglobulin G constitutes 75 % of serum immunoglobulins. Immunoglobulin G binds to viruses, bacteria, as well as fungi and facilitates their destruction or neutralization via agglutination (and thereby immobilizing them), activation of the compliment cascade, and opsinization for phagocytosis. The whole IgG molecule possesses both the F(c) region, recognized by high-affinity Fc receptor proteins, as well as the F(ab) region possessing the epitope-recognition site. Both heavy and light chains of the antibody molecule are present.
-
