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- 抗原 See all WECC products
- WECC (WecC Gene Product (WECC))
- 适用
- Pseudomonas, Bacteria
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宿主
- 山羊
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克隆类型
- 多克隆
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标记
- This WECC antibody is conjugated to HRP
- 应用范围
- ELISA, Immunoprecipitation (IP), Western Blotting (WB)
- 纯化方法
- N-Acylmannosamine-1-Dehydrogenase is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Goat Serum, and purified and partially purified N-Acylmannosamine-1-Dehydrogenase derived from Pseudomonas.
- 免疫原
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Immunogen: N-Acyl Mannosamine-1-Dehydrogenase [Recombinant Sequence from Pseudomonas expressed in E.coli ]
Immunogen Type: Native Protein
- 亚型
- IgG
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anti-WecC Gene Product (WECC) antibody (Biotin)
WECC 适用: Pseudomonas ELISA, IP, WB 宿主: 山羊 Polyclonal Biotin
anti-WecC Gene Product (WECC) antibodyWECC 适用: Pseudomonas ELISA 宿主: 山羊 Polyclonal unconjugated
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- 应用备注
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Application Note: Anti-N-Acylmannosamine-1-Dehydrogenase has been assayed against 1.0 μg of N-Acylmannosamine-1-Dehydrogenase IgG in a standard capture ELISA using ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature. A working dilution of 1:4,000 to 1:16,000 is suggested for this product.
Western Blot Dilution: 1:500 - 1:5,000
Immunoprecipitation Dilution: 1:100
ELISA Dilution: 1:5,000 - 1:20,000
- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 溶解方式
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Reconstitution Volume: 100 μL
Reconstitution Buffer: Restore with deionized water (or equivalent)
- 浓度
- 1 mg/mL
- 缓冲液
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
- 储存液
- Gentamicin sulfate
- 注意事项
- This product contains Gentamicin sulfate: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C,-20 °C
- 储存方法
- Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
- 有效期
- 12 months
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- 抗原
- WECC (WecC Gene Product (WECC))
- 别名
- N-Acylmannosamine-1-Dehydrogenase (WECC 产品)
- 背景
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Synonyms: N-acylmannosamine 1-dehydrogenase NAM-DH EC=1.1.1.233
Background: N-Acylmannosamine-1-Dehydrogenase acts on acetyl-D-mannosamine and glycolyl-D-mannosamine. This enzyme belongs to the family of oxidoreductases, specifically those acting on the CH-OH group of donor with NAD+ or NADP+ as acceptor.
Gene Name: NAM-DH
- 基因ID
- 7614553
- UniProt
- P22441
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