COL3 抗体
(1 reference)-
- 抗原 See all COL3 抗体
- COL3 (Collagen, Type III (COL3))
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适用
- 人, Cow, 哺乳动物
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宿主
- 兔
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克隆类型
- 多克隆
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标记
- This COL3 antibody is un-conjugated
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应用范围
- Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunoprecipitation (IP)
- 交叉反应 （详细）
- Typically negligible cross-reactivity against other types of collagens was detected by ELISA against purified standards. Some class-specific anti-collagens may be specific for three-dimensional epitopes which may result in diminished reactivity with denatured collagen or formalin-fixed, paraffin embedded tissues. This antibody reacts with most mammalian Type III collagens and has negligible cross-reactivity with Type I, II, IV, V or VI collagens. Non-specific cross-reaction of anti-collagen antibodies with other human serum proteins or non-collagen extracellular matrix proteins is negligible.
- 纯化方法
- Anti-Collagen Type III has been prepared by immunoaffinity chromatography using immobilized antigens followed by extensive cross-adsorption against other collagens, human serum proteins and non-collagen extracellular matrix proteins to remove any unwanted specificities.
- 免疫原
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Immunogen: Collagen Type III from human and bovine placenta
Immunogen Type: Native Protein
- 亚型
- IgG
- Top Product
- Discover our top product COL3 Primary Antibody
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anti-Collagen, Type III (COL3) antibody
COL3 适用: 人, Cow, 哺乳动物 WB, ELISA, IHC, IP 宿主: 兔 Polyclonal unconjugated
anti-Collagen, Type III (COL3) (AA 1301-1400) antibodyCOL3 适用: 人, 大鼠, 小鼠, 犬, 兔 WB, ELISA, IHC (fro), IF (cc), IF (p), IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Collagen, Type III (COL3) (AA 801-900) antibodyCOL3 适用: 人, 大鼠, 小鼠 WB, ELISA, IHC (fro), IF (cc), IF (p), IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Collagen, Type III (COL3) antibodyCOL3 适用: 人 WB, IF, IP 宿主: 兔 Monoclonal unconjugated
anti-Collagen, Type III (COL3) antibodyCOL3 适用: 人 ELISA 宿主: 山羊 Polyclonal unconjugated
anti-Collagen, Type III (COL3) antibody (Biotin)COL3 适用: 人 ELISA 宿主: 山羊 Polyclonal Biotin
anti-Collagen, Type III (COL3) antibody (HRP)COL3 适用: 人, Cow, 哺乳动物 WB, ELISA, IHC, IP 宿主: 兔 Polyclonal HRP
anti-Collagen, Type III (COL3) antibodyCOL3 适用: 人, 大鼠, 小鼠 WB 宿主: 小鼠 Monoclonal Q76 unconjugated
anti-Collagen, Type III (COL3) antibody (Magnetic Particles)COL3 适用: 人, 大鼠, 小鼠 IP 宿主: 小鼠 Monoclonal Col-29 Magnetic Particles
anti-Collagen, Type III (COL3) antibody (Biotin)COL3 适用: 人, Cow, 哺乳动物 WB, ELISA, IHC, IP 宿主: 兔 Polyclonal Biotin
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- 应用备注
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Immunohistochemistry Dilution: 1:50 - 1:200
Application Note: Anti-Collagen antibodies have been used for indirect trapping ELISA for quantitation of antigen in serum using a standard curve, immunoprecipitation, immunohistochemistry, native (non-denaturing, non-dissociating) PAGE, and western blotting for highly sensitive qualitative analysis.
Western Blot Dilution: 1:1,000 - 1:10,000
Immunoprecipitation Dilution: 1:100
ELISA Dilution: 1:5,000 - 1:50,000
- 限制
- 仅限研究用
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- 状态
- Liquid
- 溶解方式
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Reconstitution Volume: 500 μL
- 浓度
- 1.0 mg/mL
- 缓冲液
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Buffer: 0.125 M Sodium Borate, 0.075 M Sodium Chloride, 0.005 M EDTA, pH 8.0
Stabilizer: None
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C,-20 °C
- 储存方法
- Store vial at 4° C prior to opening. This product is stable at 4° C as an undiluted liquid. Dilute only prior to immediate use. For extended storage, mix with an equal volume of glycerol, aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing.
- 有效期
- 12 months
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: "Twist1 promotes heart valve cell proliferation and extracellular matrix gene expression during development in vivo and is expressed in human diseased aortic valves." in: Developmental biology, Vol. 347, Issue 1, pp. 167-79, (2010) (PubMed).
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: "Twist1 promotes heart valve cell proliferation and extracellular matrix gene expression during development in vivo and is expressed in human diseased aortic valves." in: Developmental biology, Vol. 347, Issue 1, pp. 167-79, (2010) (PubMed).
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- 抗原
- COL3 (Collagen, Type III (COL3))
- 别名
- COLLAGEN Type III (COL3 产品)
- 别名
- PT antibody, RPRGL2 antibody, THPH1 antibody, Cf-2 antibody, Cf2 antibody, FII antibody, thrombin antibody, prothrombin antibody, coagulation factor II, thrombin antibody, coagulation factor II antibody, F2 antibody
- 背景
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Synonyms: Collagen type III alpha 1 antibody, Collagen type III alpha antibody, EDS4A antibody, Ehlers Danlos syndrome type IV, autosomal dominant antibody, Fetal collagen antibody
Background: Rockland produces highly active antibodies and conjugates to collagens. Collagens are highly conserved throughout evolution and are characterized by an uninterrupted ''Glycine-X-Y'' triplet repeat that is a necessary part of the triple helical structure. For these reasons, it is often extremely difficult to generate antibodies with specificities to collagens. The development of 'type' specific antibodies is dependent on NON-DENATURED three-dimensional epitopes. Rockland extensively purifies collagens for immunization from human and bovine placenta and cartilage by limited pepsin digestion and selective salt precipitation. This preparation results in a native conformation of the protein. Antibodies are isolated from rabbit antiserum and are extensively cross-adsorbed by immunoaffinity purification to produce 'type' specific antibodies. Greatly diminished reactivity and selectivity of these antibodies will result if denaturing and reducing conditions are used for SDS-PAGE and immunoblotting.
Gene Name: COL3A1
- 基因ID
- 1281
- UniProt
- P02461
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