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- 抗原 See all COL2 抗体
- COL2 (Collagen, Type II (COL2))
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适用
- 人, Cow, 哺乳动物
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宿主
- 兔
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克隆类型
- 多克隆
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标记
- This COL2 antibody is conjugated to Biotin
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应用范围
- Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunoprecipitation (IP)
- 交叉反应 （详细）
- Typically negligible cross reactivity against other types of collagens was detected by ELISA against purified standards. Some class specific anti-collagens may be specific for three-dimensional epitopes which may result in diminished reactivity with denatured collagen or formalin-fixed, paraffin embedded tissues. This antibody reacts with most mammalian Type II collagens and has negligible cross-reactivity with Type I, III, IV, V and VI collagens. Non-specific cross reaction of anti-collagen antibodies with other human serum proteins or non-collagen extracellular matrix proteins is negligible.
- 纯化方法
- Anti-Collagen Type II has been prepared by immunoaffinity chromatography using immobilized antigens followed by extensive cross-adsorption against other collagens, human serum proteins and non-collagen extracellular matrix proteins to remove any unwanted specificities.
- 免疫原
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Immunogen: Anti-Collagen Type II antibody was produced by repeated immunizations with Collagen Type II from adult human knee cartilage and bovine nasal cartilage.
Immunogen Type: Native Protein
- 亚型
- IgG
- Top Product
- Discover our top product COL2 Primary Antibody
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anti-Collagen, Type II (COL2) (AA 1201-1300) antibody
COL2 适用: 人, 小鼠, 大鼠, 兔, 豚鼠 WB, ELISA, IHC (p), IHC (fro), FACS, IF (p), IF (cc) 宿主: 兔 Polyclonal unconjugated
anti-Collagen, Type II (COL2) antibodyCOL2 适用: 人, 小鼠, 大鼠, Cow, 哺乳动物, 绵羊 WB, ELISA, IHC, IP 宿主: 兔 Polyclonal unconjugated
anti-Collagen, Type II (COL2) antibodyCOL2 适用: 人 ELISA 宿主: 山羊 Polyclonal unconjugated
anti-Collagen, Type II (COL2) (N-Term) antibodyCOL2 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC, IF/ICC 宿主: 兔 Polyclonal unconjugated
anti-Collagen, Type II (COL2) (AA 1401-1487) antibodyCOL2 适用: 人, 大鼠, 兔 WB, ELISA, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Collagen, Type II (COL2) (AA 101-150) antibodyCOL2 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Collagen, Type II (COL2) antibodyCOL2 适用: 人, 小鼠, 大鼠 IHC (p) 宿主: 小鼠 Monoclonal 1H1 unconjugated
anti-Collagen, Type II (COL2) antibody (Biotin)COL2 适用: 人 ELISA 宿主: 山羊 Polyclonal Biotin
anti-Collagen, Type II (COL2) antibody (FITC)COL2 适用: 人, Cow, 哺乳动物 WB, ELISA, IHC, IP 宿主: 兔 Polyclonal FITC
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- 应用备注
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Immunohistochemistry Dilution: 1:200 - 1:1,000
Application Note: Anti-Collagen Type II antibody is suitable for western blotting, IP, IHC and for ELISA. Researchers should determine optimal titers for applications that are not stated below.
Western Blot Dilution: 1:5,000 - 1:10,000
Immunoprecipitation Dilution: 1:100
ELISA Dilution: 1:10,000 - 1:50,000
- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 溶解方式
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Reconstitution Volume: 100 μL
Reconstitution Buffer: Restore with deionized water (or equivalent)
- 浓度
- 1.0 mg/mL
- 缓冲液
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Buffer: 0.125 M Sodium Borate, 0.075 M Sodium Chloride, 0.005 M EDTA, pH 8.0
Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C,-20 °C
- 储存方法
- Store vial at 4° C prior to restoration. Restore with 0.1 mL of deionized water (or equivalent). For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is one (1) year from date of restoration.
- 有效期
- 12 months
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- 抗原
- COL2 (Collagen, Type II (COL2))
- 别名
- Collagen Type II (COL2 产品)
- 别名
- PT antibody, RPRGL2 antibody, THPH1 antibody, Cf-2 antibody, Cf2 antibody, FII antibody, thrombin antibody, prothrombin antibody, CAP18 antibody, Cnlp antibody, Cramp antibody, FALL39 antibody, MCLP antibody, coagulation factor II, thrombin antibody, coagulation factor II antibody, cathelicidin antimicrobial peptide antibody, Cuticle collagen 2 antibody, collagen type II alpha 1 chain antibody, F2 antibody, Camp antibody, col-2 antibody, COL2A1 antibody
- 背景
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Synonyms: Cartilage collagen antibody, Chondrocalcin antibody, COL11A3 antibody, COL2A1 antibody
Background: Anti-Collagen Type II antibody detects Type-II collagen which is the basis for articular cartilage and hyaline cartilage. It makes up 50 % of all protein in cartilage and 85-90 % of collagen of articular cartilage. Type II collagen does form fibrils which allow cartilage to entrap the proteoglycan aggregate as well as provide tensile strength to the tissue. Anti-Collagen Type II antibody is ideal for investigators involved in Immunology, Neuroscience and Signal Transduction research.
Gene Name: COL2A1
- 基因ID
- 1280
- UniProt
- P02458
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