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CRISPR-Cas9 (C-Term) 抗体 (HRP)

适用: Streptococcus pyogenes WB, ELISA, FM 宿主: 兔 Polyclonal HRP
产品编号 ABIN5596703
发货至: 中国
  • 抗原
    CRISPR-Cas9
    抗原表位
    • 6
    • 2
    • 1
    • 1
    • 1
    C-Term
    适用
    • 19
    • 2
    • 1
    • 1
    • 1
    • 1
    Streptococcus pyogenes
    宿主
    • 15
    • 10
    克隆类型
    • 13
    • 12
    多克隆
    标记
    • 18
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    HRP
    应用范围
    • 23
    • 9
    • 8
    • 6
    • 5
    • 4
    • 3
    • 2
    • 1
    • 1
    • 1
    • 1
    Western Blotting (WB), ELISA, Fluorescence Microscopy (FM)
    纯化方法
    Anti-Cas9 antibody is directed against Streptococcus pyogenes Cas-9. The product was affinity purified from monospecific antiserum by immunoaffinity chromatography.
    免疫原

    Immunogen: Cas-9 affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to a region near the C-terminal of Streptococcus pyogenes Cas-9 protein.

    Immunogen Type: Peptide

    亚型
    IgG
  • 应用备注

    Application Note: Cas9 Peroxidase Conjugated Antibody has been tested for use in ELISA, Immunofluorescence, and western blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band ~158 kDa in size corresponding to Cas-9 protein by western blotting in the appropriate cell lysate or extract.

    Western Blot Dilution: 1 μg/mL

    ELISA Dilution: 1:25,000-1:30,000

    IF Microscopy Dilution: 1 μg/mL

    说明

    User Optimized

    限制
    仅限研究用
  • 状态
    Lyophilized
    溶解方式

    Reconstitution Volume: 100 μL

    Reconstitution Buffer: Restore with deionized water (or equivalent)

    浓度
    1.0 mg/mL
    缓冲液

    Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

    Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

    储存液
    Gentamicin sulfate
    注意事项
    This product contains Gentamicin sulfate: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    储存条件
    4 °C,-20 °C
    储存方法
    Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
    有效期
    12 months
  • 抗原
    CRISPR-Cas9
    别名
    Cas 9
    背景

    Synonyms: CRISPR, CRISPR-associated endonuclease Cas9/Csn1, SpyCas9, csn1, HRP, Cas-9 Antibody

    Background: The Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR) and CRISPR Associated (Cas) system was discovered in bacteria as a defense against foreign DNA, even being believed to act as a sort of prokaryotic immune system. The Cas9 protein is becoming a a useful tool in the field of genomic editing for its ability to induce site-directed double strand breaks in DNA. It can cleave almost any sequence complementary to the guide RNA and has recently been used for modifying the genome of human embryos for the first time.

    Gene Name: cas9

    基因ID
    901176
    NCBI登录号
    NP_269215
    UniProt
    Q99ZW2
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