Western Blotting (WB), ELISA, Immunoprecipitation (IP), Immunofluorescence (IF), Immunohistochemistry (IHC), Dot Blot (DB)
原理
Mouse monoclonal antibody raised against synthetic peptide of His tag.
特异性
Recognizes the epitope of 6x His-tags encoded by many commercially available vectors, regardless of the tag's location in the fusion protein sequences (i.e.: reacts with N-terminal, C-terminal or internal 6x His-tags).
免疫原
A synthetic peptide (conjugated with KLH) corresponding to His tag.