HIF3A 抗体 (AA 581-592)
(1 reference)-
- 抗原 See all HIF3A 抗体
- HIF3A (Hypoxia Inducible Factor 3, alpha Subunit (HIF3A))
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抗原表位
- AA 581-592
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适用
- 小鼠, 大鼠
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宿主
- 兔
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克隆类型
- 多克隆
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标记
- This HIF3A antibody is un-conjugated
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应用范围
- Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)
- 序列
- Mouse Hif3 ?, 662 residue, predicted MW 73.0 kDa 1 mdwdqdrsnt elrkeksrda arsrrsqete vlyqlahtlp fargvsahld kasimrltis 61 ylrmhrlcaa gewnqvekgg epldacylka legfvmvlta egdmaylsen vskhlglsql 121 elighsifdf ihpcdqeelq daltprpnls kkkleapter hfslrmkstl tsrgrtlnlk 181 aatwkvlhcs ghmraykppa qtspagsprs epplqclvli ceaiphpasl epplgrgafl 241 srhsldmkft ycderiaeva gyspddligc sayeyihald sdavsrsiht llskgqavtg 301 qyrflartgg ylwtqtqatv vsggrgpqse siicvhflis rveetgvvls leqteqhtrr 361 pprlsassqk gipgnsvdsp aprilaflhp palseaslaa dprrfcspdl rrlmapildg 421 pppaatpstp qatrrpqspl padlpdklav glenahrlst aqknktvetd ldiaqdsdtl 481 dlemlapyis mdddfqlnss eqlpkvhrrp prvarrprar sfhglsppip epsllprwgs 541 dprlncssps rgdrptaslm pgtrkralaq ssedkglell eikppkrspr lepgsfllpp 601 lslsfllqgr qllgnqqdpr aplvhshepl glapsllslc qheetvqprn hfppaaglgq 661 th
- 特异性
- This antibody recognizes Mouse HIF3A (581-592). A BLAST analysis was used to suggest cross reactivity with HIF3A protein from Mouse (100 % homology) and Rat (91 % homology). Reactivity against human HIF3A is not expected as nlz 75 % homology for the immunizing sequence is noted. No reactivity is expected against other forms of HIF proteins.
- 交叉反应 （详细）
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Species reactivity (expected):Rat.
Species reactivity (tested):Mouse. - 纯化方法
- Immunoaffinity chromatography.
- 免疫原
- Synthetic peptide corresponding to aa 581-592 of mouse Hif3a
- Top Product
- Discover our top product HIF3A Primary Antibody
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anti-Hypoxia Inducible Factor 3, alpha Subunit (HIF3A) antibody
HIF3A 适用: 小鼠, 大鼠 IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Hypoxia Inducible Factor 3, alpha Subunit (HIF3A) (AA 581-592) antibodyHIF3A 适用: 人, 小鼠 WB, ELISA, IHC, IF, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Hypoxia Inducible Factor 3, alpha Subunit (HIF3A) (AA 516-669) antibodyHIF3A 适用: 人 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-Hypoxia Inducible Factor 3, alpha Subunit (HIF3A) (AA 1-669) antibodyHIF3A 适用: 人 WB, IF 宿主: 小鼠 Polyclonal unconjugated
anti-Hypoxia Inducible Factor 3, alpha Subunit (HIF3A) (AA 355-669) antibodyHIF3A 适用: 人 WB, IHC, IHC (p) 宿主: 小鼠 Monoclonal 4C2 unconjugated
anti-Hypoxia Inducible Factor 3, alpha Subunit (HIF3A) (AA 581-592) antibodyHIF3A 适用: 小鼠 WB, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Hypoxia Inducible Factor 3, alpha Subunit (HIF3A) (C-Term) antibodyHIF3A 适用: 人 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Hypoxia Inducible Factor 3, alpha Subunit (HIF3A) (AA 1-363) antibodyHIF3A 适用: 人 WB 宿主: 小鼠 Polyclonal unconjugated
anti-Hypoxia Inducible Factor 3, alpha Subunit (HIF3A) (Internal Region) antibodyHIF3A 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-Hypoxia Inducible Factor 3, alpha Subunit (HIF3A) (N-Term) antibodyVerified HIF3A 适用: 人 ELISA, IHC 宿主: 山羊 Polyclonal unconjugated
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- 应用备注
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ELISA (1/14,000-1/80,000). Westen blot (1/1,500-1/8,000), Expect a band approximately 72 kDa in size correspondingto Hif3 α protein by western blotting in the appropriate cell lysate or extract.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. - 限制
- 仅限研究用
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- 浓度
- 1.2 mg/mL (by UV absorbance at 280 nm)
- 缓冲液
- 0.02 M Potassium Phosphate, 0.12 M Sodium Chloride, pH 7.2, 0.01 % (w/v) Sodium Azide, None
- 储存液
- Sodium azide
- 注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 注意事项
- Avoid repeated freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. Dilute only prior to immediate use
- 储存条件
- 4 °C
- 储存方法
- Store vial at 2 - 8 °C prior to reconstitution. Following reconstitution the product is stable for one month at 2 - 8 °C as an undiluted liquid. . For extended storage add glycerol to 50 % and then aliquot contents and freeze at -20 °C or below.
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: "[Mortality analyses for rungsten miners.]" in: Zhonghua lao dong wei sheng zhi ye bing za zhi = Zhonghua laodong weisheng zhiyebing zazhi = Chinese journal of industrial hygiene and occupational diseases, Vol. 26, Issue 1, pp. 24-8, (2008) (PubMed).
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: "[Mortality analyses for rungsten miners.]" in: Zhonghua lao dong wei sheng zhi ye bing za zhi = Zhonghua laodong weisheng zhiyebing zazhi = Chinese journal of industrial hygiene and occupational diseases, Vol. 26, Issue 1, pp. 24-8, (2008) (PubMed).
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- 抗原
- HIF3A (Hypoxia Inducible Factor 3, alpha Subunit (HIF3A))
- 别名
- HIF3A / HIF3 alpha (HIF3A 产品)
- 别名
- HIF-3A antibody, IPAS antibody, MOP7 antibody, PASD7 antibody, bHLHe17 antibody, Ipas antibody, NEPAS antibody, Mop7 antibody, HIF3A antibody, hypoxia inducible factor 3 alpha subunit antibody, hypoxia inducible factor 3, alpha subunit antibody, HIF3A antibody, Hif3a antibody
- 背景
- One of the most important factors in the cellular response to hypoxia is hypoxia-inducible factor (HIF), which transcriptionally activates genes encoding proteins that mediate adaptive responses to reduced oxygen availability. HIF is a heterodimer consisting of one of three subunits(HIF1-α, HIF2-α, or HIF3-α) bound to the aryl hydrocarbon receptor nuclear translocator(ARNT) that is also known as HIF1-β. HIF-α is a member of the basic helix-loop-helix (bHLH) superfamily, in which the HLH domain mediates subunit dimerization while the basic domain binds to DNA. HIF target genes play critical roles in metabolism, angiogenesis, cell proliferation, and cell survival, in fact, HIF3-α may be a marker for tumor growth and angiogenesis. Examples of HIF target genes include VEGF, glucose transporter 1 (GLUT1), and EPO. HIF binds to the hypoxia-responsive element, which contains the core recognition sequence 5'-TACGTG-3', in the cis-regulatory regions of hypoxia-inducible genes. Transcriptional activation by HIF is linked to its ability to recruit coactivator proteins such as CREB-binding protein (CBP), p300, steroid receptor coactivator-1, and translation initiation factor 2.Synonyms: BHLHE17, Basic-helix-loop-helix-PAS protein MOP7, Class E basic helix-loop-helix protein 17, HIF-3 alpha, Hypoxia-inducible factor 3 alpha, IPAS, Inhibitory PAS domain protein, MOP7, Member of PAS protein 7, PASD7
- 基因ID
- 64344
- NCBI登录号
- NP_690007
- UniProt
- Q9Y2N7
- 途径
- Warburg Effect
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