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- 抗原 See all CDT2/RAMP (DTL) 抗体
- CDT2/RAMP (DTL) (Denticleless E3 Ubiquitin Protein Ligase Homolog (DTL))
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抗原表位
- AA 229-256
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适用
- 人, 小鼠
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宿主
- 兔
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克隆类型
- 多克隆
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标记
- This CDT2/RAMP antibody is un-conjugated
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应用范围
- Western Blotting (WB), Flow Cytometry (FACS)
- 纯化方法
- This antibody is purified through a protein A column, followed by peptide affinity purification.
- 免疫原
- This DTL antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 229-256 amino acids from the Central region of human DTL.
- 亚型
- Ig Fraction
- Top Product
- Discover our top product DTL Primary Antibody
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anti-Denticleless E3 Ubiquitin Protein Ligase Homolog (DTL) (AA 393-550) antibody
DTL 适用: 人 ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-Denticleless E3 Ubiquitin Protein Ligase Homolog (DTL) (AA 1-730) antibodyDTL 适用: 人 WB, IF 宿主: 兔 Polyclonal unconjugated
anti-Denticleless E3 Ubiquitin Protein Ligase Homolog (DTL) (AA 229-256), (Middle Region) antibodyDTL 适用: 人, 小鼠 WB, FACS, EIA 宿主: 兔 Polyclonal unconjugated
anti-Denticleless E3 Ubiquitin Protein Ligase Homolog (DTL) (AA 182-231) antibodyDTL 适用: 人, 小鼠, 大鼠, Cow, 犬, 豚鼠, 马, 兔, Bat, 猴, Pig WB 宿主: 兔 Polyclonal unconjugated
anti-Denticleless E3 Ubiquitin Protein Ligase Homolog (DTL) (AA 541-730) antibodyDTL 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
anti-Denticleless E3 Ubiquitin Protein Ligase Homolog (DTL) (AA 229-256) antibodyDTL 适用: 人, 小鼠 WB, FACS 宿主: 兔 Polyclonal RB24420 unconjugated
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- 应用备注
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For WB starting dilution is: 1:1000
For FACS starting dilution is: 1:10~50 - 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- 0.5 mg/mL
- 缓冲液
- Supplied in PBS with 0.09 % (W/V) sodium azide.
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C,-20 °C
- 储存方法
- Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
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- 抗原
- CDT2/RAMP (DTL) (Denticleless E3 Ubiquitin Protein Ligase Homolog (DTL))
- 别名
- DTL (DTL 产品)
- 别名
- CDT2 antibody, DCAF2 antibody, L2DTL antibody, RAMP antibody, 2810047L02Rik antibody, 5730564G15Rik antibody, Ramp antibody, RGD1310439 antibody, cdt2 antibody, cdt2-a antibody, dcaf2 antibody, l2dtl antibody, ramp antibody, cb151 antibody, chunp6871 antibody, wu:fb54b02 antibody, denticleless E3 ubiquitin protein ligase homolog antibody, denticleless E3 ubiquitin protein ligase antibody, denticleless E3 ubiquitin protein ligase homolog S homeolog antibody, denticleless E3 ubiquitin protein ligase homolog (Drosophila) antibody, DTL antibody, Dtl antibody, dtl.S antibody, dtl antibody
- 背景
- Substrate-specific adapter of a DCX (DDB1-CUL4-X-box) E3 ubiquitin-protein ligase complex required for cell cycle control, DNA damage response and translesion DNA synthesis. The DCX(DTL) complex, also named CRL4(CDT2) complex, mediates the polyubiquitination and subsequent degradation of CDT1 and CDKN1A/p21(CIP1). CDT1 degradation in response to DNA damage is necessary to ensure proper cell cycle regulation of DNA replication. CDKN1A/p21(CIP1) degradation during S phase or following UV irradiation is essential to control replication licensing. Most substrates require their interaction with PCNA for their polyubiquitination: substrates interact with PCNA via their PIP-box, and those containing the 'K+4' motif in the PIP box, recruit the DCX(DTL) complex, leading to their degradation. In undamaged proliferating cells, the DCX(DTL) complex also promotes the 'Lys-164' monoubiquitination of PCNA, thereby being involved in PCNA-dependent translesion DNA synthesis.
- 分子量
- 79 kDa
- 基因ID
- 51514
- UniProt
- Q9NZJ0
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