This antibody is purified through a protein A column, followed by peptide affinity purification.
免疫原
This DENND1B antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 39-68 amino acids from the N-terminal region of human DENND1B.
DENND1B
适用: 小鼠
WB, IF (cc), IF (p)
宿主: 兔
Polyclonal
Alexa Fluor 350
应用备注
For WB starting dilution is: 1:500
For IHC-P starting dilution is: 1:50~100
For FACS starting dilution is: 1:10~50
限制
仅限研究用
状态
Liquid
浓度
0.5 mg/mL
缓冲液
Supplied in PBS with 0.09 % (W/V) sodium azide.
储存液
Sodium azide
注意事项
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
储存条件
4 °C,-20 °C
储存方法
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Clathrin (see MIM 118955)-mediated endocytosis is a major mechanism for internalization of proteins and lipids. Members of the connecdenn family, such as DENND1B, function as guanine nucleotide exchange factors (GEFs) for the early endosomal small GTPase RAB35 (MIM 604199) and bind to clathrin and clathrin adaptor protein-2 (AP2, see MIM 601024). Thus, connecdenns link RAB35 activation with the clathrin machinery (Marat and McPherson, 2010 [PubMed 20154091]).