Mouse monoclonal antibody raised against native Kinesin (heavy chain).
特异性
This antibody recognizes heavy chain of conventional kinesin associated withvesicles and with lower affinity with denaturated molecule. Epitope is located in coiled-coilstalk domain. It stains Western blots of kinesin-enriched preparations. Epitope mapping (by limited proteolysis of partially purified porcine kinesin) followed by immunoblotting has revealed that antibodies MAB3620, MAB3621 and MAB3622 react with differentsets of fragments. This antibody does not react with kinesin bound to taxol-stabilized microtubules.
The optimal working dilution should be determined by the end user.
限制
仅限研究用
状态
Liquid
浓度
1 mg/mL
缓冲液
In PBS, pH 7.4 (0.09 % sodium azide)
储存液
Sodium azide
注意事项
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
储存条件
4 °C
储存方法
Store at 4°C. Do not freeze.
Malcová-Janatová, Richterová, Dráber, Hasek: "Preparation of human recombinant kinesin heavy chain and epitope mapping of its structural domains." in: Folia microbiologica, Vol. 49, Issue 6, pp. 665-70, (2005) (PubMed).
Mac?rek, Dráberová, Richterová, Böhm, Dráber: "Monoclonal antibodies KN-02 and KN-03 against the heavy chain of kinesin." in: Hybridoma and hybridomics, Vol. 21, Issue 6, pp. 457-62, (2003) (PubMed).
抗原
Kinesin (heavy chain)
别名
KINH antibody, KNS antibody, KNS1 antibody, UKHC antibody, kinesin family member 5B antibody, kinesin family member 5C antibody, KIF5B antibody, Kif5c antibody