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CD16 抗体

CD16 适用: 人, Non-Human Primate FACS, IP, IHC (fro) 宿主: 小鼠 Monoclonal 3G8 unconjugated

Flow cytometry surface staining pattern of human peripheral whole blood stained using anti-human CD16 (3G8) purified antibody (concentration in sample 2 μg/mL, GAM APC).

Surface staining of CD16 in human peripheral blood with anti-CD16 (3G8) purified / GAM-APC.

Expression profiling on peripheral blood subsets using anti-human CD16 purified antibody (clone 3G8).

Separation of human CD16 positive lymphocytes (red-filled) from CD16 negative lymphocytes (black-dashed) in flow cytometry analysis (surface staining) of peripheral whole blood stained using anti-human CD16 (3G8) purified antibody (concentration in sample 2 μg/mL, GAM APC).

Anti-human CD16 purified antibody (clone 3G8) works in flow cytometry application.

5 images

(4 references)

产品编号 ABIN509571

发货至: 中国

Datasheet as PDF

抗原 See all CD16 抗体

CD16
适用
127

26

20

20

20

8

8

8

7

4

2

2

2

2

2

2

2

1

1

1
人, Non-Human Primate
宿主
110

29

14

1
小鼠
Compatible Secondaries
克隆类型
126

26

1
单克隆
标记
55

18

13

12

7

7

3

3

2

2

2

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
This CD16 antibody is un-conjugated
应用范围
124

30

28

25

24

23

20

17

6

5

3

3

3

2

2

2

1
Flow Cytometry (FACS), Immunoprecipitation (IP), Immunohistochemistry (Frozen Sections) (IHC (fro))

特异性

The mouse monoclonal antibody 3G8 recognizes an extracellular epitope of CD16, a low affinity receptor for aggregated IgG (FcgammaRIII antigen). CD16 exists in two different isoforms: CD16a (FcgammaRIIIA, 50-65 kDa, expressed on NK-cells, monocytes and macrophages) and CD16b (FcgammaRIIIB, 48 kDa, mainly expressed on neutrophils). Regarding CD16-158V/F polymorphism, the antibody 3G8 detects both 158V and 158F allotype on natural NK cells.

交叉反应（详细）

Human, Non-Human Primates

纯化方法

Purified by protein-A affinity chromatography.

纯度

> 95 % (by SDS-PAGE)

免疫原

Human neutrophils

克隆位点

3G8

亚型

IgG1 kappa
anti-CD16 (CD16) antibody (FITC)
CD16 适用: 人, Non-Human Primate FACS 宿主: 小鼠 Monoclonal LNK16 FITC

anti-CD16 (CD16) antibody
CD16 适用: 人 FACS, IP, WB 宿主: 小鼠 Monoclonal MEM-154 unconjugated

anti-CD16 (CD16) antibody
CD16 适用: 人, Pig, Non-Human Primate FACS 宿主: 小鼠 Monoclonal MEM-168 unconjugated

anti-CD16 (CD16) antibody
CD16 适用: 人 FACS, IP, WB, Func 宿主: 小鼠 Monoclonal MEM-154 unconjugated

anti-CD16 (CD16) antibody (Biotin)
CD16 适用: 人 FACS, IP, WB 宿主: 小鼠 Monoclonal MEM-154 Biotin

anti-CD16 (CD16) (AA 17-208) antibody
CD16 适用: 人 FACS, WB, IHC, ELISA, ICC, Neut 宿主: 小鼠 Monoclonal 2G10A9 unconjugated

anti-CD16 (CD16) antibody
CD16 适用: 人, Non-Human Primate FACS, IP, Func, IHC (fro) 宿主: 小鼠 Monoclonal 3G8 unconjugated

anti-CD16 (CD16) antibody (PE-DyLight 594)
CD16 适用: 人, Non-Human Primate FACS 宿主: 小鼠 Monoclonal 3G8 PE-DyLight 594

anti-CD16 (CD16) antibody
CD16 适用: 人 WB, IHC (p) 宿主: 小鼠 Monoclonal 2B1 unconjugated

anti-CD16 (CD16) antibody
CD16 适用: 人 FACS 宿主: 小鼠 Monoclonal GRM1 unconjugated

应用备注

Flow cytometry: Recommended dilution: 6 μg/mL.
Immunohistochemistry (frozen sections): Acetone fixation.

限制

仅限研究用
浓度

1 mg/mL

缓冲液

Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

储存液

Sodium azide

注意事项

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

注意事项

Do not freeze.

储存条件

4 °C

储存方法

Store at 2-8°C. Do not freeze.
Choi, Wang, Peterson, Letvin, Reimann: "Use of an anti-CD16 antibody for in vivo depletion of natural killer cells in rhesus macaques." in: Immunology, Vol. 124, Issue 2, pp. 215-22, (2008) (PubMed).

Congy-Jolivet, Bolzec, Ternant, Ohresser, Watier, Thibault: "Fc gamma RIIIa expression is not increased on natural killer cells expressing the Fc gamma RIIIa-158V allotype." in: Cancer research, Vol. 68, Issue 4, pp. 976-80, (2008) (PubMed).

Wijngaarden, van Roon, van de Winkel, Bijlsma, Lafeber: "Down-regulation of activating Fcgamma receptors on monocytes of patients with rheumatoid arthritis upon methotrexate treatment." in: Rheumatology (Oxford, England), Vol. 44, Issue 6, pp. 729-34, (2005) (PubMed).

Metes, Ernst, Chambers, Sulica, Herberman, Morel: "Expression of functional CD32 molecules on human NK cells is determined by an allelic polymorphism of the FcgammaRIIC gene." in: Blood, Vol. 91, Issue 7, pp. 2369-80, (1998) (PubMed).
抗原

CD16

别名

CD16 (CD16 产品)

别名

Fcgr3 antibody, Fcgr3a antibody, CD16 antibody, FCGR3 antibody, FCGR3@ antibody, CD16b antibody, FCG3 antibody, FCR-10 antibody, FCRIII antibody, FCRIIIb antibody, FcRIII antibody, Fc fragment of IgG, low affinity IIa, receptor antibody, Fc fragment of IgG, low affinity IIIa, receptor (CD16a) antibody, Fc fragment of IgG receptor IIIb antibody, Fc fragment of IgG receptor IIIa antibody, Fc receptor, IgG, low affinity III antibody, Fc-gamma RIII antibody, Fcgr2a antibody, FCGR3A antibody, FCGR3B antibody, Fcgr3 antibody, FCGR3 antibody

背景

CD16 (FcgammaRIII) is a 50-65 kDa glycoprotein serving as a low affinity IgG receptor. Human FcgammaRIII is expressed in two forms –, FcgammaRIII-A and -B. FcgammaRIII-A is a transmembrane protein of monocytes, macrophages, NK cells and a subset of T cells. It is associated with FcepsilonRI-gamma subunit and is responsible for antibody-dependent NK cell cytotoxicity. Mast cell FcgammaRIII-A is associated, moreover, with FcepsilonRI-beta subunit. Besides IgG, FcgammaRIII-A can be triggered also by oligomeric IgE. FcgammaRIII-B is a GPI-linked monomeric receptor expressed on neutrophils and is involved in their activation and induction of a proadhesive phenotype.,FcgammaRIII, IGFR3, FCRIII

途径

Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process

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