HSP70 抗体 (HRP)
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- 抗原 See all HSP70 抗体
- HSP70 (Heat Shock Protein 70 (HSP70))
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适用
- 人
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宿主
- 小鼠
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克隆类型
- 单克隆
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标记
- This HSP70 antibody is conjugated to HRP
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应用范围
- Western Blotting (WB), Immunofluorescence (IF), Immunocytochemistry (ICC), Flow Cytometry (FACS)
- 特异性
- Induced by heat shock.,Conformation-spec ab for mem-bound HSP70. Binds to cell surface HSP70 w/o permeabilization of cell mem in tumor cells. Does't bind to Intra-C HSP70 at low conc. X-reacts with HSC70. ~70 kDa in WB.
- 交叉反应
- Cow, 人, 小鼠, Pig, 大鼠
- 纯化方法
- Protein G Purified
- 免疫原
- Human native HSP70 protein
- 克隆位点
- 1H11
- 亚型
- IgG1
- Top Product
- Discover our top product HSP70 Primary Antibody
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anti-Heat Shock Protein 70 (HSP70) antibody
HSP70 适用: 人 WB, ELISA, IHC, IF, ICC, FACS, AA, IEM, BI 宿主: 小鼠 Monoclonal C92F3A-5 unconjugated
anti-Heat Shock Protein 70 (HSP70) antibodyHSP70 适用: 人 WB, ELISA, IHC, IF, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Heat Shock Protein 70 (HSP70) antibody (HRP)HSP70 适用: 人 WB, ELISA, IHC, IF, IP, ICC 宿主: 兔 Polyclonal HRP
anti-Heat Shock Protein 70 (HSP70) antibody (FITC)HSP70 适用: 人 WB, ELISA, IHC, IF, IP, ICC 宿主: 兔 Polyclonal FITC
anti-Heat Shock Protein 70 (HSP70) antibody (Alkaline Phosphatase (AP))HSP70 适用: 人 WB, ELISA, IHC, IF, IP, ICC 宿主: 兔 Polyclonal Alkaline Phosphatase (AP)
anti-Heat Shock Protein 70 (HSP70) antibody (Biotin)HSP70 适用: 人 WB, ELISA, IHC, IF, IP, ICC 宿主: 兔 Polyclonal Biotin
anti-Heat Shock Protein 70 (HSP70) antibody (PE)HSP70 适用: 人 WB, ELISA, IHC, IF, IP, ICC 宿主: 兔 Polyclonal PE
anti-Heat Shock Protein 70 (HSP70) antibody (APC)HSP70 适用: 人 WB, ELISA, IHC, IF, IP, ICC 宿主: 兔 Polyclonal APC
anti-Heat Shock Protein 70 (HSP70) antibody (PerCP)HSP70 适用: 人 WB, ELISA, IHC, IF, IP, ICC 宿主: 兔 Polyclonal PerCP
anti-Heat Shock Protein 70 (HSP70) antibody (Atto 488)HSP70 适用: 人 WB, ELISA, IHC, IF, IP, ICC 宿主: 兔 Polyclonal Atto 488
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- 应用备注
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- WB (1:1000)
- ICC/IF (1:100)
- FACS (1:250)
- optimal dilutions for assays should be determined by the user.
- 说明
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A 1:250 dilution of ABIN5066942 was sufficient for detection of cell surface HSP70 in HCT116 cells using Fluorescence-activated cell sorting, applied in flow cytometry, with FITC as the fluorescent probe.
- 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- 0.5 mg/mL
- 缓冲液
- PBS pH 7.4, 50 % glycerol, 0.09 % Sodium azide, Storage buffer may change when conjugated
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C
- 储存方法
- Conjugated antibodies should be stored at 4°C
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- 抗原
- HSP70 (Heat Shock Protein 70 (HSP70))
- 别名
- HSP70 (HSP70 产品)
- 别名
- APG-2 antibody, HS24/P52 antibody, HSPH2 antibody, RY antibody, hsp70 antibody, hsp70RY antibody, CG31354 antibody, HSP70 antibody, Hsp70Bb antibody, hsp70B antibody, hsp70Bb-prime antibody, DmelCG5834 antibody, CG5834 antibody, HSPA1 antibody, HSP70B' antibody, HSPA6 antibody, ARABIDOPSIS HEAT SHOCK PROTEIN 70 antibody, ATHSP70 antibody, heat shock protein 70 antibody, LOC100305036 antibody, hsc70 antibody, Hsp70 antibody, Hsp70-1 antibody, Hsp70.1 antibody, hsp68 antibody, Hsp110 antibody, irp94 antibody, HSP70-2 antibody, HSPA1B antibody, HSPA2 antibody, hsp70-5 antibody, HSP70-1 antibody, HSP70.1 antibody, HSP70.2 antibody, heat shock protein family A (Hsp70) member 4 antibody, CG5834 gene product from transcript CG5834-RA antibody, heat shock protein 70 antibody, heat shock protein family A (Hsp70) member 6 antibody, heat shock 70kDa protein 2 antibody, heat shock 70 kD protein cognate antibody, Hsp70 family chaperone antibody, Heat shock protein 70 antibody, Heat shock protein 70, putative antibody, heat shock protein 1B antibody, heat shock protein family A member 4 antibody, heat shock 70kDa protein 1A antibody, heat shock protein 1 antibody, Heat Shock Protein antibody, heat shock cognate 70-kd protein antibody, Heat shock 70 kDa protein 1A antibody, HSPA4 antibody, Hsp70Bbb antibody, HSP70 antibody, HSPA6 antibody, HSPA2 antibody, PCC7424_2419 antibody, Isop_1041 antibody, CGB_C3390W antibody, Bacsa_1698 antibody, dnaK-B antibody, LOC100305036 antibody, Hspa1b antibody, Hspa4 antibody, HSPA1A antibody, hsp1 antibody, hsp-70 antibody, hsp70 antibody, LOC108348108 antibody
- 背景
- HSP70 is a highly conserved protein that is ubiquitously expressed. It can be found in chloroplasts, eukaryotic cytosol, endoplasmic reticulum, and mitochondria, but also embedded in the cell membrane and in the extracellular space (1,2,3,4). Even though HSP70 is one of the most studied heat shock proteins, the export mechanism and method of membrane insertion are not fully understood. The majority of the proteins in the heat shock family lack a consensus signal for secretion via the ER-Golgi pathway (5). Researchers have found that HSP70 may be released from cells via exosomes or secretory vesicles (6). Although HSP70 is ubiquitously expressed, there is not much information about its presence on cell surface. The finding that HSP70 is localized on the cell surface of cancer cells but not normal cells suggests a conformational change of HSP70 in the lower pH environment characteristic of cancer cells (7). The presence of cell membrane embedded HSP70 has been found to increase the stability of cancer cells, thereby protecting tumors from environmental stress (8, 9). Anti-HSP70 antibody, clone 1H11 (Catalog No. SMC-249) is unique from other commercially available antibodies in that it can bind to the extracellular region of the cell membrane embedded HSP70 protein, allowing researchers to differentiate between membrane bound and intracellular HSP70 across cancer cells types.
- 基因ID
- 3303
- NCBI登录号
- NP_005336
- UniProt
- P0DMV8, P0DMV9
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