MAP1LC3A 抗体 (AA 1-12) (HRP)
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- 抗原 See all MAP1LC3A 抗体
- MAP1LC3A (Microtubule-Associated Protein 1 Light Chain 3 alpha (MAP1LC3A))
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抗原表位
- AA 1-12
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适用
- 人
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宿主
- 兔
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克隆类型
- 多克隆
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标记
- This MAP1LC3A antibody is conjugated to HRP
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应用范围
- Western Blotting (WB), Immunofluorescence (IF), Immunocytochemistry (ICC)
- 特异性
- Most abundant in heart, brain, liver, skeletal muscle and testis but absent in thymus and peripheral blood leukocytes.,Detects ~14 kDa. Band at 50 kDa is LC3A complex.
- 交叉反应
- 人, 小鼠, 大鼠
- 纯化方法
- Peptide Affinity Purified
- 免疫原
- Synthetic peptide from the N-terminal of Human LC3A (aa. 1-12)
- 亚型
- IgG
- Top Product
- Discover our top product MAP1LC3A Primary Antibody
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- 应用备注
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- WB (1:1000)
- ICC/IF (1:100)
- optimal dilutions for assays should be determined by the user.
- 说明
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A 1:1000 dilution of ABIN5065916 was sufficient for detection of LC3A in 15 μg of Human HeLa Cell Lysates by ECL immunoblot analysis using goat anti-rabbit IgG:HRP as the secondary antibody.
- 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- 1 mg/mL
- 缓冲液
- PBS, 50 % glycerol, 0.09 % sodium azide, Storage buffer may change when conjugated
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C
- 储存方法
- Conjugated antibodies should be stored at 4°C
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- 抗原
- MAP1LC3A (Microtubule-Associated Protein 1 Light Chain 3 alpha (MAP1LC3A))
- 别名
- LC3A (MAP1LC3A 产品)
- 别名
- ATG8E antibody, LC3 antibody, LC3A antibody, MAP1ALC3 antibody, MAP1BLC3 antibody, 1010001H21Rik antibody, 4922501H04Rik antibody, LC3a antibody, MGC69006 antibody, zgc:77094 antibody, MGC89867 antibody, MAP1LC3A antibody, map1lc3a antibody, microtubule associated protein 1 light chain 3 alpha antibody, microtubule-associated protein 1 light chain 3 alpha antibody, microtubule associated protein 1 light chain 3 alpha S homeolog antibody, MAP1LC3A antibody, Map1lc3a antibody, map1lc3a.S antibody, map1lc3a antibody
- 背景
- Light chain 3 (LC3) is a microtubule-associated protein with an approximate molecular mass of 17 kDa, and can be found ubiquitously throughout mammalian tissue. LC3 plays a role in autophagy, once the autophagic process is initiated in a cell, LC3 is conjugated to phosphatidylethanolamine to form LC3-II. This molecule is recruited to the autophagosome at the time of fusion with lysosomes, and LC3-II in autolysosomal lumen is degraded. Therefore, monitoring LC3 is an important tool for detecting autophagy and autophagy-related processes. LC3A is one of three isoforms which exhibits abundant expression in the heart, brain, liver, skeletal muscle, and testis but is absent in thymus and peripheral blood leukocytes.
- 基因ID
- 84557
- NCBI登录号
- NP_115903
- UniProt
- Q9H492
- 途径
- Autophagy
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