Western Blot: 1/500 - 1/1000. Immunohistochemistry: 1/50 - 1/200. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
限制
仅限研究用
浓度
1,0 mg/mL
缓冲液
Phosphate buffered saline (PBS), pH 7.2., 15 mM sodium azide
储存液
Sodium azide
注意事项
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
注意事项
Avoid repeated freezing and thawing.
储存条件
4 °C/-20 °C
储存方法
Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
AI481586 antibody, C81621 antibody, RGD1305179 antibody, wu:fi03b05 antibody, zgc:66437 antibody, NEDD4 binding protein 1 antibody, Nedd4 binding protein 1 antibody, nedd4 binding protein 1 antibody, nedd4 binding protein 1 L homeolog antibody, N4BP1 antibody, N4bp1 antibody, n4bp1 antibody, n4bp1.L antibody
背景
Nedd4-binding partner-1 (N4BP1) has been identified as a protein interactor and a substrate of the homologous to E6AP C terminus (HECT) domain-containing E3 ubiquitin-protein ligase (E3), Nedd4. Here, we describe a previously unrecognized functional interaction between N4BP1 and Itch, a Nedd4 structurally related E3, which contains four WW domains, conferring substrate-binding activity. We show that N4BP1 association with the second WW domain (WW2) of Itch interferes with E3 binding to its substrates. In particular, we found that N4BP1 and p73alpha, a target of Itchmediated ubiquitin/proteasome proteolysis, share the same binding site. By competing with p73alpha for binding to the WW2 domain, N4BP1 reduces the ability of Itch to recruit and ubiquitylate p73alpha and inhibits Itch autoubiquitylation activity both in in vitro and in vivo ubiquitylation assays.Synonyms: KIAA0615, NEDD4-binding protein 1