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- 抗原 See all EIF5B 抗体
- EIF5B (Eukaryotic Translation Initiation Factor 5B (EIF5B))
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适用
- 人, 小鼠, 大鼠
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宿主
- 兔
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克隆类型
- 多克隆
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标记
- This EIF5B antibody is un-conjugated
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应用范围
- Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
- 特异性
- This antibody detects endogenous levels of eIF5B protein. (region surrounding His1086)
- 交叉反应 （详细）
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Species reactivity (expected):Mouse and Rat.
Species reactivity (tested):Human. - 纯化方法
- Affinity chromatography
- 纯度
- > 95 % by SDS-PAGE
- Top Product
- Discover our top product EIF5B Primary Antibody
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anti-Eukaryotic Translation Initiation Factor 5B (EIF5B) antibody
EIF5B 适用: 人, 小鼠, 大鼠 WB, IF 宿主: 兔 Polyclonal unconjugated
anti-Eukaryotic Translation Initiation Factor 5B (EIF5B) (AA 1-1220) antibodyEIF5B 适用: 人 WB, IF 宿主: 小鼠 Polyclonal unconjugated
anti-Eukaryotic Translation Initiation Factor 5B (EIF5B) (AA 1-273) antibodyEIF5B 适用: 人 WB, IF 宿主: 兔 Polyclonal unconjugated
anti-Eukaryotic Translation Initiation Factor 5B (EIF5B) (AA 1121-1218) antibodyEIF5B 适用: 人 WB, ELISA 宿主: 小鼠 Polyclonal unconjugated
anti-Eukaryotic Translation Initiation Factor 5B (EIF5B) (C-Term) antibodyEIF5B 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Eukaryotic Translation Initiation Factor 5B (EIF5B) (AA 1121-1218) antibodyEIF5B 适用: 人 ELISA 宿主: 小鼠 Monoclonal 3F9 unconjugated
anti-Eukaryotic Translation Initiation Factor 5B (EIF5B) (AA 557-606) antibodyEIF5B 适用: 人, 犬, 马, 猴 WB 宿主: 兔 Polyclonal unconjugated
anti-Eukaryotic Translation Initiation Factor 5B (EIF5B) (Middle Region) antibodyEIF5B 适用: 人, 犬, 马, Cow, Pig, 兔 WB 宿主: 兔 Polyclonal unconjugated
anti-Eukaryotic Translation Initiation Factor 5B (EIF5B) (C-Term) antibodyEIF5B 适用: 人, 小鼠, 大鼠 WB, IHC, IP 宿主: 兔 Polyclonal unconjugated
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- 应用备注
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Western Blot: 1/500 - 1/1000. Immunohistochemistry: 1/50 - 1/200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. - 限制
- 仅限研究用
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- 浓度
- 1,0 mg/mL
- 缓冲液
- Phosphate buffered saline (PBS), pH 7.2., 0.05 % sodium azide
- 储存液
- Sodium azide
- 注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 注意事项
- Avoid repeated freezing and thawing.
- 储存条件
- 4 °C/-20 °C
- 储存方法
- Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
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- 抗原
- EIF5B (Eukaryotic Translation Initiation Factor 5B (EIF5B))
- 别名
- EIF5B / IF2 (EIF5B 产品)
- 别名
- CIF2 antibody, EIF5B antibody, MGC134193 antibody, im:6912504 antibody, si:ch211-254d18.3 antibody, AO090003000953 antibody, DDBDRAFT_0206214 antibody, DDBDRAFT_0234259 antibody, DDB_0206214 antibody, DDB_0234259 antibody, IF2 antibody, A030003E17Rik antibody, BC018347 antibody, eukaryotic translation initiation factor 5B antibody, translation initiation factor IF-2 antibody, EIF5B antibody, eif5b antibody, EDI_190850 antibody, AOR_1_1690154 antibody, CMU_007690 antibody, POSPLDRAFT_134975 antibody, POSPLDRAFT_134953 antibody, eIF5b antibody, MCYG_03474 antibody, PITG_20386 antibody, eif5B antibody, MGYG_03082 antibody, PGTG_13938 antibody, Eif5b antibody
- 背景
- Accurate initiation of translation in eukaryotes is complex and requires many factors, some of which are composed of multiple subunits. The process is simpler in prokaryotes which have only three initiation factors (IF1, IF2, IF3). Two of these factors are conserved in eukaryotes: the homolog of IF1 is eIF1A and the homolog of IF2 is eIF5B. This gene encodes eIF5B. Factors eIF1A and eIF5B interact on the ribosome along with other initiation factors and GTP to position the initiation methionine tRNA on the start codon of the mRNA so that translation initiates accurately.Synonyms: Eukaryotic translation initiation factor 5B, KIAA0741, Translation initiation factor IF-2, eIF-5B
- 分子量
- approx. 139 kDa
- 基因ID
- 9669
- NCBI登录号
- NP_056988
- UniProt
- O60841
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