Western Blot: 1/500 - 1/1000. Immunohistochemistry: 1/50 - 1/200. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
CUGBP1 antibody, BRUNOL2 antibody, CUG-BP antibody, CUGBP antibody, EDEN-BP antibody, NAB50 antibody, NAPOR antibody, hNab50 antibody, 1600010O03Rik antibody, AA407467 antibody, Brunol2 antibody, CUG-BP1 antibody, Cugbp1 antibody, D2Wsu101e antibody, HNAB50 antibody, Bruno-like antibody, brul antibody, cb920 antibody, cugbp1 antibody, brunol2 antibody, cug-bp antibody, cug-bp1 antibody, cugbp antibody, cugbp1-a antibody, eden-bp antibody, edenbp antibody, nab50 antibody, CUGBP Elav-like family member 1 antibody, CUGBP, Elav-like family member 1 antibody, cugbp, Elav-like family member 1 antibody, CUGBP Elav-like family member 1 L homeolog antibody, CELF1 antibody, Celf1 antibody, celf1 antibody, celf1.L antibody
背景
Myotonic dystrophy (DM) is an autosomal dominant neuromuscular disease that is associated with a (CTG)n repeat expansion in the 3'-untranslated region of the myotonin protein kinase gene (DMPK). CUG-BP1 and CUG-BP2 are proteins that bind specifically to (CUG)8 oligonucleotides in vitro. While CUG-BP1 has the major binding activity in normal cells, nuclear CUG-BP2 binding activity increases in DM cells. Both CUG-BP1 and CUG-BP2 are isoforms of a novel heterogeneous nuclear ribonucleoprotein (hnRNP), hNab50. CUG-BP1, an RNA CUG triplet repeat binding protein, regulates splicing and translation of various RNAs. Expansion of RNA CUG repeats in the DMPK in DM is associated with alterations in binding activity of CUG-BP1 as well as alterations in the translation of the C/EBPbeta transcription factor. CUG-BP1 is an important regulator of initiation from different AUG codons of C/EBPbeta mRNA. In normal cells, CUG-BP1 up-regulates the p21 protein during differentiation by inducing the translation of p21 via binding to a GC-rich sequence located within the 5' region of p21 mRNA. In DM cells, failure to accumulate CUG-BP1 leads to a reduction of p21 and alterations in other proteins responsible for cell cycle withdrawal.Synonyms: 50 kDa nuclear polyadenylated RNA-binding protein, BRUNOL2, Bruno-like protein 2, CELF-1, CELF1, CUG triplet repeat RNA-binding protein 1, CUG-BP- and ETR-3-like factor 1, CUGBP, Deadenylation factor CUG-BP, Embryo deadenylation element-binding protein homolog, NAB50, RNA-binding protein BRUNOL-2