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Rhodopsin 抗体

RHO 适用: 人, Cow, 大鼠, 小鼠, Pig WB, IHC, ICC, IF 宿主: 小鼠 Monoclonal B630 unconjugated
产品编号 ABIN4956273
发货至: 中国
  • 抗原 See all Rhodopsin (RHO) 抗体
    Rhodopsin (RHO)
    适用
    • 39
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    人, Cow, 大鼠, 小鼠, Pig
    宿主
    • 39
    • 26
    • 2
    • 1
    • 1
    小鼠
    克隆类型
    • 35
    • 33
    • 1
    单克隆
    标记
    • 28
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    This Rhodopsin antibody is un-conjugated
    应用范围
    • 52
    • 42
    • 35
    • 25
    • 14
    • 12
    • 10
    • 8
    • 2
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    Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF)
    纯化方法
    affinity purified antibody
    克隆位点
    B630
    亚型
    IgG1
    Top Product
    Discover our top product RHO Primary Antibody
  • 应用备注
    Try at dilutions of ~1:1,000 for immunofluorescence. For western blots try at 1:5,000. A suitable control tissue is retinal extracts. Rhodopsin run at 35 kDa on SDS-PAGE gels.
    限制
    仅限研究用
  • 状态
    Liquid
    浓度
    1 mg/mL
    储存液
    Sodium azide
    注意事项
    This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    注意事项
    Avoid repeated freezing and thawing.
    储存条件
    4 °C/-20 °C
    储存方法
    Store at 4°C short term or -20°C long term.
  • 抗原
    Rhodopsin (RHO)
    别名
    Rhodopsin (RHO 产品)
    别名
    CSNBAD1 antibody, OPN2 antibody, RP4 antibody, RH1 antibody, Rh antibody, fi06d11 antibody, wu:fi06d11 antibody, zfo2 antibody, zfrho antibody, Noerg1 antibody, Opn2 antibody, Ops antibody, opn2 antibody, rh1 antibody, rp4 antibody, xrho antibody, PIGRHO1 antibody, ops antibody, RDP1 antibody, RHO antibody, rhodopsin antibody, rhodopsin L homeolog antibody, RHO antibody, Rho antibody, rho antibody, rho.L antibody
    物质类
    Chemical
    背景
    Rhodopsin is the protein in the mammalian retina responsible for the light sensitivity of rod cells, which are responsible for vision in low light levels. Somewhat surprisingly, the rhodopsin protein turned out to be a typical member of the seven transmembrane G protein-coupled receptor (GPCR) superfamily. Whereas other GPCRs initiate signaling on binding a specific ligand, rhodopsin exists with a ligand already bound, specifically the vitamin A related substance retinal. Retinal can exist in two isomeric forms, 11-cis and 11-trans retinal. In the dark rhodopsin is associated with 11-cis retinal, but photons cause the 11-cis form to flip to the 11-trans form, and this causes an alteration in the structure of the rhodopsin making it catalytically active. Activated rhodopsin in turn activates the GTP binding protein transducin by favoring the loss of GDP and the addition of GTP. Transducin is a typical member of the family of heterotrimeric G proteins, and consists of an alpha, and a beta,gamma, subunit. The alpha, subunit is responsible for the GTP binding and the GTP bound form activates a phosphodiesterase (PDE) enzyme which hydrolyses cyclic GMP. This in turn results in the closure of cyclic nucleotide gated ion channels in the rod cell membrane, which prevent the influx of sodium and calcium ions. This results in an increase in the membrane potential of the rod cell and so reduces the rate of synaptic signaling. So light stimulation results in a reduced rate of photoreceptor synaptic release. This information is transmitted through neurons of the retina to the visual centers of the brain (see review 1, 2). Rhodopsin activity is shut off by phosphorylation under the influence of rhodopsin kinase, the activity of which results in binding of visual arrestin, which prevents rhodopsin from interacting with and activating more transducin molecules. This basic signaling paradigm proved to be a prototype for understanding how other GPCRs function, as proteins similar to transducin, arrestin and rhodopsin kinase are found in these pathways. MCA-B630 was raised against bovine outer segment discs and shows convincing staining for rhodopsin both on western blots and on sections of retina. The protein is concentrated in rod outer segments. The HGNC name for this protein is RHO.
    途径
    WNT signaling, Sensory Perception of Sound, Regulation of G-Protein Coupled Receptor Protein Signaling, Phototransduction
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