Recombinant 兔 anti-人 IgG4 Antibody
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- 抗原 See all IgG4 products
- IgG4
- 抗体类型
- Recombinant Antibody
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适用
- 人
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宿主
- 兔
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克隆类型
- 单克隆
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应用范围
- Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Flow Cytometry (FACS), Immunocytochemistry (ICC)
- 产品特性
- This recombinant Human IgG4 antibody reacts to the heavy chain of hIgG4. No cross reactivity with human IgG1, IgG2, IgG3, IgM, IgA, IgD, IgE, mouse/rat/goat IgG.
- 纯化方法
- Protein A purified from animal origin-free supernatant
- 免疫原
- Peptide corresponding to the hinge region of human IgG4 was used as the immunogen for this recombinant Human IgG4 antibody.
- 克隆位点
- RM120
- 亚型
- IgG
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- 应用备注
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The stated application concentrations are suggested starting points. Titration of the recombinant Human IgG4 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
1. A pH 6 Citrate buffer or pH 9 Tris/EDTA buffer HIER step is recommended for testing of FFPE tissue sections.\. ELISA: 50 ng/well - 200 ng/well (Capture), 0.05-0.2 μg/mL (Detection),Western Blot: 0.5-2 μg/mL,Immunocytochemistry: 1-10 μg/mL,Immunohistochemistry: 1-10 μg/mL (1)
- 限制
- 仅限研究用
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- 缓冲液
- 1 mg/mL in PBS with 50 % glycerol, 1 % BSA and 0.09 % sodium azide
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- -20 °C
- 储存方法
- Store the recombinant Human IgG4 antibody at -20°C (with glycerol) or aliquot and store at -20°C (without glycerol).
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