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TNF alpha 抗体 (C-Term)

Name: TNF alpha 抗体 (C-Term)
SKU: ABIN4886747
Availability: OutOfStock

TNF alpha 适用: 人, 小鼠, 大鼠 WB, IHC (p) 宿主: 兔 Polyclonal unconjugated

(38 references)

产品编号 ABIN4886747

发货至: 中国

Datasheet as PDF

抗原 See all TNF alpha 抗体

TNF alpha (Tumor Necrosis Factor alpha (TNF alpha))
抗原表位
47

32

15

15

14

12

10

6

6

6

5

5

4

3

2

2

2

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1
AA 201-233, C-Term
适用
323

181

122

67

50

42

27

26

23

16

12

10

10

9

6

5

5

2

2

1

1

1

1
人, 小鼠, 大鼠
宿主
261

193

31

25

6

2

2
兔
Compatible Secondaries
克隆类型
277

234
多克隆
标记
248

86

45

20

17

12

6

6

6

6

6

6

6

6

6

5

4

3

3

3

3

3

3

2

2

2

2

1

1

1

1

1
This TNF alpha antibody is un-conjugated
应用范围
347

210

129

122

74

62

57

49

48

40

39

38

37

32

20

12

10

9

9

6

6

6

5

4

3

3

3

2

2

2

1

1

1

1

1

1

1

1

1
Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

原理

Rabbit IgG polyclonal antibody for Tumor necrosis factor(TNF) detection. Tested with WB, IHC-P in Human,Mouse,Rat.

序列

QLEKGDRLSA EINRPDYLDF AESGQVYFGI IAL

交叉反应（详细）

No cross reactivity with other proteins.

产品特性

Rabbit IgG polyclonal antibody for Tumor necrosis factor(TNF) detection. Tested with WB, IHC-P in Human,Mouse,Rat.
Gene Name: tumor necrosis factor
Protein Name: Tumor necrosis factor

纯化方法

Immunogen affinity purified.

免疫原

A synthetic peptide corresponding to a sequence at the C-terminus of human TNF alpha(201-233aa QLEKGDRLSAEINRPDYLDFAESGQVYFGIIAL), different from the related mouse sequence by five amino acids, and rat sequence by seven amino acids.

亚型

IgG

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TNF alpha 适用: 人, 小鼠, 大鼠, 兔, 马 WB, ELISA, IHC (p), IF (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated

anti-Tumor Necrosis Factor alpha (TNF alpha) antibody
TNF alpha 适用: 人, 大鼠 IHC, FACS, IF, StM 宿主: 小鼠 Monoclonal TNFA-1172 unconjugated

anti-Tumor Necrosis Factor alpha (TNF alpha) (AA 1-233) antibody
TNF alpha 适用: 人 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated

anti-Tumor Necrosis Factor alpha (TNF alpha) (AA 77-233) antibody
TNF alpha 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated

anti-Tumor Necrosis Factor alpha (TNF alpha) (AA 1-233) antibody
TNF alpha 适用: 人 WB, ELISA, IHC (p) 宿主: 小鼠 Monoclonal M1-C4 unconjugated

anti-Tumor Necrosis Factor alpha (TNF alpha) antibody (FITC)
TNF alpha 适用: 人, Pig, Non-Human Primate FACS 宿主: 小鼠 Monoclonal MAb11 FITC

anti-Tumor Necrosis Factor alpha (TNF alpha) (AA 80-227) antibody
TNF alpha 适用: 小鼠 WB 宿主: 兔 Polyclonal unconjugated

anti-Tumor Necrosis Factor alpha (TNF alpha) (AA 86-150) antibody
TNF alpha 适用: 人, 小鼠, 大鼠, Pig, 兔 WB, ELISA, IHC (p), IF (p), IHC (fro), IF (cc) 宿主: 兔 Polyclonal unconjugated

anti-Tumor Necrosis Factor alpha (TNF alpha) antibody
TNF alpha 适用: 人, 小鼠 WB, IHC (p), IF (p) 宿主: 小鼠 Monoclonal 1F6 unconjugated

anti-Tumor Necrosis Factor alpha (TNF alpha) (AA 80-235) antibody
TNF alpha 适用: 小鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated

应用备注

WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Human, Mouse, The detection limit for TNF is approximately 2.5 ng/lane under reducing conditions.
IHC-P: Concentration: 0.5-1 μg/mL, Tested Species: Human, Mouse, Rat, Epitope Retrieval by Heat: Boiling the paraffin sections in 10 mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections.
Notes: Tested Species: Species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. Other applications have not been tested. Optimal dilutions should be determined by end users.

说明

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

限制

仅限研究用
状态

Lyophilized

溶解方式

Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

浓度

500 μg/mL

缓冲液

Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.

储存液

Sodium azide, Thimerosal (Merthiolate)

注意事项

This product contains Sodium azide and Thimerosal (Merthiolate): POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

注意事项

Avoid repeated freezing and thawing.

储存条件

4 °C/-20 °C

储存方法

At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.
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抗原

TNF alpha (Tumor Necrosis Factor alpha (TNF alpha))

别名

TNF (TNF alpha 产品)

别名

DIF antibody, TNF-alpha antibody, TNFA antibody, TNFSF2 antibody, RATTNF antibody, Tnfa antibody, tnf antibody, TNF-a antibody, TNFalpha antibody, Tnfsf1a antibody, TNFa antibody, cTNF antibody, Tnf-alpha antibody, tnfa-like antibody, TNF-ALPHA antibody, dif antibody, tnfa antibody, xtnf antibody, tnfsf2 antibody, tnf-alpha antibody, Cachectin antibody, tumor necrosis factor antibody, tumor necrosis factor b (TNF superfamily, member 2) antibody, tumor necrosis factor alpha antibody, tumor necrosis factor a (TNF superfamily, member 2) antibody, TNF antibody, Tnf antibody, tnf antibody, tnfb antibody, tnf-alpha antibody, LOC103694380 antibody, tnfa antibody

背景

TNF alpha(Tumor Necrosis Factor alpha) gene encodes a multifunctional proinflammatory cytokine that belongs to the tumor necrosis factor(TNF) superfamily. This cytokine is mainly secreted by macrophages. It can bind to, and thus functions through its receptors TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. This cytokine is involved in the regulation of a wide spectrum of biological processes including cell proliferation, differentiation, apoptosis, lipid metabolism, and coagulation. This cytokine has been implicated in a variety of diseases, including autoimmune diseases, insulin resistance, and cancer. Knockout studies in mice also suggested the neuroprotective function of this cytokine.

Synonyms: APC1 antibody|APC1 protein antibody|Cachectin antibody|DIF antibody|Differentiation inducing factor antibody|Macrophage cytotoxic factor antibody|MCF antibody|Necrosin antibody|TNF a antibody|TNF alpha antibody|TNF antibody|TNF Macrophage Derived antibody|TNF Monocyte Derived antibody|TNF Superfamily Member 2 antibody|TNF superfamily, member 2 antibody|TNF, macrophage derived antibody|TNF, monocyte derived antibody|TNF-a antibody|TNF-alpha antibody|TNFA antibody|TNFSF2 antibody|Tumor necrosis factor alpha antibody|Tumor necrosis factor antibody|Tumor necrosis factor ligand superfamily member 2 antibody|Tumor Necrosis Factor Precursor antibody|Tumor Necrosis Factor, Membrane Form antibody|Tumor necrosis factor, soluble form antibody|Tumour Necrosis Factor Alpha antibody

UniProt

P01375

途径

NF-kappaB Signaling, Apoptosis, Caspase Cascade in Apoptosis, TLR signaling, Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Positive Regulation of Endopeptidase Activity, Hepatitis C, Protein targeting to Nucleus, Inflammasome