E2F4 抗体 (N-Term)
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- 抗原 See all E2F4 抗体
- E2F4 (E2F Transcription Factor 4, P107/p130-Binding (E2F4))
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抗原表位
- AA 106-144, N-Term
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适用
- 人, 小鼠, 大鼠
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宿主
- 兔
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克隆类型
- 多克隆
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标记
- This E2F4 antibody is un-conjugated
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应用范围
- Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
- 原理
- Rabbit IgG polyclonal antibody for Transcription factor E2F4(E2F4) detection. Tested with WB, IHC-P in Human,Mouse,Rat.
- 序列
- ELQQREQELD QHKVWVQQSI RNVTEDVQNS CLAYVTHED
- 交叉反应 (详细)
- No cross reactivity with other proteins.
- 产品特性
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Rabbit IgG polyclonal antibody for Transcription factor E2F4(E2F4) detection. Tested with WB, IHC-P in Human,Mouse,Rat.
Gene Name: E2F transcription factor 4, p107/p130-binding
Protein Name: Transcription factor E2F4 - 纯化方法
- Immunogen affinity purified.
- 免疫原
- A synthetic peptide corresponding to a sequence at the N-terminus of human E2F4 (106-144aa ELQQREQELDQHKVWVQQSIRNVTEDVQNSCLAYVTHED), identical to the related mouse and rat sequences.
- 亚型
- IgG
- Top Product
- Discover our top product E2F4 Primary Antibody
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- 应用备注
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WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Human
IHC-P: Concentration: 0.5-1 μg/mL, Tested Species: Human, Mouse, Rat, Epitope Retrieval by Heat: Boiling the paraffin sections in 10 mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections.
Notes: Tested Species: Species with positive results. Other applications have not been tested. Optimal dilutions should be determined by end users. - 说明
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Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).
- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 溶解方式
- Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
- 浓度
- 500 μg/mL
- 缓冲液
- Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 注意事项
- Avoid repeated freezing and thawing.
- 储存条件
- 4 °C/-20 °C
- 储存方法
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At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.
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- 抗原
- E2F4 (E2F Transcription Factor 4, P107/p130-Binding (E2F4))
- 别名
- E2F4 (E2F4 产品)
- 别名
- E2F-4 antibody, 2010111M04Rik antibody, AI427446 antibody, E2F4 antibody, e2f-4 antibody, fb72f07 antibody, wu:fb72f07 antibody, wu:fe05f06 antibody, zgc:63815 antibody, E2F transcription factor 4 antibody, E2F transcription factor 4 S homeolog antibody, E2F4 antibody, E2f4 antibody, e2f4.S antibody, e2f4 antibody
- 背景
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Transcription factor E2F4 is a protein that in humans is encoded by the E2F4 gene. The protein encoded by this gene is a member of the E2F family of transcription factors. This gene is mapped to 16q22.1. The E2F family plays a crucial role in the control of cell cycle and action of tumor suppressor proteins and is also a target of the transforming proteins of small DNA tumor viruses. These domains include a DNA binding domain, a dimerization domain which determines interaction with the differentiation regulated transcription factor proteins (DP), a transactivation domain enriched in acidic amino acids, and a tumor suppressor protein association domain which is embedded within the transactivation domain. This protein binds to all three of the tumor suppressor proteins pRB, p107 and p130, but with higher affinity to the last two. Additionally, it plays an important role in the suppression of proliferation-associated genes, and its gene mutation and increased expression may be associated with human cancer.
Synonyms: E2F4 | E2F-4 | Transcription factor E2F4 | Q16254 - 基因ID
- 1874
- UniProt
- Q16254
- 途径
- Cell Division Cycle, Mitotic G1-G1/S Phases, Regulation of Cell Size
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