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EGF 抗体

EGF 适用: 人 WB, ELISA, Neut 宿主: 兔 Polyclonal unconjugated
产品编号 ABIN465547
发货至: 中国
  • 抗原 See all EGF 抗体
    EGF (Epidermal Growth Factor (EGF))
    适用
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    宿主
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    克隆类型
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    多克隆
    标记
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    This EGF antibody is un-conjugated
    应用范围
    • 207
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    Western Blotting (WB), ELISA, Neutralization (Neut)
    特异性
    Human EGF
    纯化方法
    Immunoaffinity purified
    免疫原
    E.coli derived recombinant Human EGF.

    Type of Immunogen: Cell extract
    Top Product
    Discover our top product EGF Primary Antibody
  • 应用备注
    Approved: ELISA (0.5 - 2 μg/mL), Neut (0.25 - 0.35 μg/mL), WB

    Usage: Immunohistochemistry: This antibody stained formalin-fixed, paraffin-embedded sections of normal human skin. The recommended concentration is 1 μg/mL-0.25 μg/mL overnight at 4 °C. An HRP-labeled polymer detection system was used with a non-alcohol soluble AEC chromogen and a proteinase K antigen retrieval. Optimal concentrations and conditions may vary. Neutralization: To yield one-half maximal inhibition [ND] of the biological activity of hEGF (25 pg/mL), a concentration of < 0.1 μg/mL of this antibody is required. ELISA: To detect hEGF by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5-2 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Human EGF (LS-C104809) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant hEGF. Western Blot: To detect hEGF by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hEGF is 1.5-3 ng/lane, under either reducing or non-reducing conditions.
    说明

    Target Species of Antibody: Human

    限制
    仅限研究用
  • 状态
    Lyophilized
    溶解方式
    Sterile water
    浓度
    Lot specific
    缓冲液
    Lyophilized from PBS,  pH 7.2
    注意事项
    Avoid freeze/thaw cycles.
    储存条件
    4 °C,-20 °C
    储存方法
    Store Lyophilized at room temperature up to 1 month
    Reconstituted for up to 2 weeks at 2-8°C. Aliquot and freeze at -20°C for long term storage. Avoid freeze/thaw cycles.
  • 抗原
    EGF (Epidermal Growth Factor (EGF))
    别名
    EGF (EGF 产品)
    别名
    HOMG4 antibody, URG antibody, AI790464 antibody, CEGF antibody, epidermal growth factor antibody, pro-epidermal growth factor antibody, EGF antibody, egf antibody, CpipJ_CPIJ020278 antibody, Egf antibody
    背景
    Name/Gene ID: EGF

    Synonyms: EGF, Beta-urogastrone, HOMG4, URG, Epidermal growth factor, Pro-epidermal growth factor
    基因ID
    1950
    UniProt
    P01133
    途径
    NF-kappaB Signaling, RTK signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Regulation of Carbohydrate Metabolic Process, Hepatitis C, Protein targeting to Nucleus, Interaction of EGFR with phospholipase C-gamma, Thromboxane A2 Receptor Signaling, EGFR Downregulation
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