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MIA2 抗体

MIA2 适用: 人 WB, ELISA 宿主: 兔 Polyclonal unconjugated
产品编号 ABIN464847
发货至: 中国
  • 抗原 See all MIA2 抗体
    MIA2 (Melanoma Inhibitory Activity Protein 2 (MIA2))
    适用
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    宿主
    • 48
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    克隆类型
    • 48
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    多克隆
    标记
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    This MIA2 antibody is un-conjugated
    应用范围
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    Western Blotting (WB), ELISA
    特异性
    Human MIA-2
    纯化方法
    Immunoaffinity purified
    免疫原
    E.coli derived recombinant Human MIA-2.

    Type of Immunogen: Cell extract
    Top Product
    Discover our top product MIA2 Primary Antibody
  • 应用备注
    Approved: ELISA, WB (0.1 - 0.2 μg/mL)

    Usage: Immunohistochemistry: This antibody stained formalin-fixed paraffin-embedded sections of human pancreas infiltrating ductal adenocarcinoma tissue. The recommended concentration is 5 μg/mL with an overnight incubation at 4 °C. An HRP-labeled polymer detection system was used with an alcohol-soluble AEC chromogen. Optimal results for these conditions were achieved without antigen retrieval. Optimal concentrations and conditions may vary. ELISA: To detect hMIA-2 by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5-2 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Human MIA-2 (LS-C104686) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant hMIA-2. Western Blot: To detect hMIA-2 by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hMIA-2 is 1.5-3 ng/lane, under either reducing or non-reducing conditions.
    说明

    Target Species of Antibody: Human

    限制
    仅限研究用
  • 状态
    Lyophilized
    溶解方式
    Sterile water
    浓度
    Lot specific
    缓冲液
    Lyophilized from PBS,  pH 7.2
    注意事项
    Avoid freeze/thaw cycles.
    储存条件
    4 °C,-20 °C
    储存方法
    Store Lyophilized at room temperature up to 1 month
    Reconstituted for up to 2 weeks at 2-8°C. Aliquot and freeze at -20°C for long term storage. Avoid freeze/thaw cycles.
  • 抗原
    MIA2 (Melanoma Inhibitory Activity Protein 2 (MIA2))
    别名
    MIA2 (MIA2 产品)
    背景
    Name/Gene ID: MIA2
    Family: MIA

    Synonyms: MIA2, Melanoma inhibitory activity 2
    基因ID
    117153
    UniProt
    Q96PC5
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