Human Papilloma Virus 18 E2 (HPV-18 E2) (AA 1-83) 抗体
(1 validation)-
- 抗原
- Human Papilloma Virus 18 E2 (HPV-18 E2)
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抗原表位
- AA 1-83
-
适用
- Human Papillomavirus (HPV)
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宿主
- 小鼠
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克隆类型
- 单克隆
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标记
- 非结合性
- 应用范围
- ELISA, Western Blotting (WB), Immunofluorescence (IF)
- 特异性
- Reacts with HPV-18 E2-protein.
- 纯化方法
- purified with Protein G affinity chromatorgaphy
- 免疫原
- Recombinant full-length HPV-18 E2-protein purifiedfrom E. coli
- 克隆位点
- 2E7
- 亚型
- IgG
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- 应用备注
- ELISA:1: 2 000, WB: 1: 1000. This antibody does not work in IP. Monoclonal antibody working titer has to be established practically for each particular antigen and assay format
- 限制
- 仅限研究用
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- by
- NanoTemper Technologies
- No.
- #102723
- 日期
- 2018.11.05
- 抗原
- HPV-18
- Lot Number
- A10P2E71-001
- Method validated
- Unfolding Profile
- Positive Control
- ABIN457548
- Negative Control
- Notes
Passed. ABIN457548 showed a clear unfolding profile with two detected unfolding events with Tis at 78.4°C and 84.4°C. It is not unusual for antibodies to show more than one unfolding transition, as they are composed of distinct domains that may unfold independently and at different temperatures. This result suggests that the protein is properly folded and functional.
- Primary Antibody
- ABIN457548
- Secondary Antibody
- Full Protocol
- Dilute ABIN457548 1:10 in PBS buffer (Roth, 1058.1, lot 285231988) to get a final volume of 15µl at a concentration of 0.67µM.
- Load sample into Tycho capillary (NanoTemper Technologies, TY-C001).
- Run Tycho measurement.
- Experimental Notes
Tycho is designed to run quick and precise protein quality check experiments. Tycho uses intrinsic protein fluorescence to follow protein unfolding while running a fast thermal ramp, yielding results in 3min. A protein’s unfolding behavior is characterized by various parameters, most notably the inflection temperature (Ti). The Ti can be used to identify properly folded protein, to compare different batches, or to analyze the influence of storage/transport conditions on a protein. An absence of Ti would suggest that the protein is already unfolded and therefore most likely nonfunctional.
生效 #102723 (Unfolding Profile)!['独立验证'标志]( "成功验证")
!['独立验证'标志]( "成功验证")
Validation Images![Unfolding profile of ABIN457548. The fluorescence signal is plotted against temperature. The native (folded) protein has a low signal at the beginning of the experiment, which increases upon unfolding, showing two unfolding events. The vertical lines indicate the Tis at 78.4 °C and 84.4 °C.]()
Unfolding profile of ABIN457548. The fluorescence signal is plotted against temperature. The native (folded) protein has a low signal at the beginning of the experiment, which increases upon unfolding, showing two unfolding events. The vertical lines indicate the Tis at 78.4 °C and 84.4 °C.
Full Methods -
- 状态
- Liquid
- 浓度
- 1 mg/ml
- 缓冲液
- PBS pH 7.4, with 0.1%
- 储存液
- Sodium azide
- 注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 注意事项
- Divide antibody into aliquots prior usage. Avoid multiple freeze-thaw cycles.
- 储存条件
- -20 °C
- 储存方法
- This product is shipped in non-frozen liquid form in ambient conditions
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- 抗原
- Human Papilloma Virus 18 E2 (HPV-18 E2)
- 物质类
- Viral Protein
- 背景
- Human papillomaviruses(HPV) are small DNA viruses whichinfect epithelia of the skin and mucosa. Over 90 types have been identified andthey mostly cause a variety of benign lesions such as warts and verrucae.However, some subtypes, notably types 16 and 18, 31 and 33, have been confirmedas agents which cause cervical cancer. Human Papillomavirus (HPV) E7 proteins havecellular transforming and trans-activating activities
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