小鼠 anti-人 IgE Antibody (Biotin)
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- 抗原 See all IgE products
- IgE
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适用
- 人
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宿主
- 小鼠
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克隆类型
- 单克隆
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标记
- Biotin
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应用范围
- ELISA, Western Blotting (WB), Flow Cytometry (FACS), Functional Studies (Func)
- 特异性
- Human IgE, Fce3-4, no cross-ractivity with other human or murine Ab isotypes
- 灵敏度
- Reactivity > 95 %, compared to calibrator (Internal Standard mAb clone BSW17), tested by ELISA binding to immobilized human Standard IgE (clone: SUS-11)
- 产品特性
- IGHE, chromosome 14q32.33
- 纯化方法
- Affinity - purified from cell culture supernatant by Immunoaffinity Chromatography on CH-Sepharose 4B - rat monoclonal anti- mouse Ck
- 纯度
- > 85 %, tested by analytical Gel Permeation Chromatography
- 免疫原
- purified human myeloma IgE
- 克隆位点
- BSW17-NBS01
- 亚型
- IgG1 kappa
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Mouse anti-Human IgE (Fc Region) antibody (Biotin)
ELISA Monoclonal HP6029 IgG2a Biotin
Mouse anti-Human IgE antibody (FITC)FACS Monoclonal BE5 IgG1 FITC
Mouse anti-Human IgE antibody (PE)FACS Monoclonal BE5 IgG1 PE
Mouse anti-Human IgE (Fc Region) antibodyELISA, WB, FACS, Func Monoclonal Le27-NBS01 IgG1 kappa unconjugated
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- 应用备注
- When used for functional cellular assays at dilutions < 1:100, the Na-azide free formulation (Cat. No. 0910-1-010 / 050-B) is recommended
- 限制
- 仅限研究用
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- 浓度
- 1 mg/mL
- 缓冲液
- PBS, pH=7.2, 0.1 % NaN3
- 储存液
- Sodium azide
- 注意事项
- WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
- 注意事项
- Aliquot to Avoid repeated freezing and thawing.
- 储存条件
- 4 °C
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- 抗原
- IgE
- Abstract
- IgE 产品
- 背景
- The antibody recognizes a conformational epitope within the Ce3 domain of human IgE spanning towards the amino-terminal part of the Ce4 domain. It blocks the binding of IgE to its high affinity receptor on mast cells and basophils. It is therefore suited as an inhibitory reference compound for mast cell modulator profiling and basic research on IgE mediated mast cell receptor signaling.
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