TYRO3 抗体 (TYRO3 Protein Tyrosine Kinase) (AA 100-250)

Details for Product anti-TYRO3 Antibody No. ABIN440474
抗原
抗原表位
AA 100-250, Internal Region
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适用
人, 小鼠
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宿主

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克隆类型
多克隆
标记
This TYRO3 antibody is un-conjugated
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应用范围
Flow Cytometry (FACS), Immunocytochemistry (ICC), Immunofluorescence (IF), Western Blotting (WB)
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选项
免疫原 Synthetic peptide made to an internal portion of the human TYRO3 protein (within residues 100-250). [Swiss-Prot# Q06418]
纯化方法 Immunogen affinity purified
抗原
别名 Dtk/TYRO3 (TYRO3 Antibody 摘要)
背景 Gene Symbol: TYRO3
分子量 Theoretical MW: 96 kDa
基因ID 7301
UniProt Q06418
途径 RTK signaling
应用备注 Western Blot 2 μg/mL, Flow Cytometry, Immunocytochemistry/Immunofluorescence 1:200This TYRO3 antibody is useful for Immunocytochemistry/Immunofluorescence and Western blot, where a band can be seen at ~96 kDa. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. Use in FLOW cytometry reported in scientific literature ( PMID 27034374).
说明

The antibodies are intended for use in vitro experiments only. Our antibodies have not been tested nor are recommended for use in vivo.

实验流程 Protocol specific for TYRO3 Antibody Procedure Guide for NBP1-28635 - Tyro3 AntibodyProcedure Guide for NBP1-28635- Tyro3 AntibodyWestern Blot Protocol
1. Perform SDS-PAGE (4-12 % MOPS) on samples to be analyzed, loading 40 µg of total protein per lane.
. Transfer proteins to Nitrocellulose according to the instructions provided by the manufacturer of the transferapparatus.
. Rinse membrane with dH2O and then stain the blot using Ponceau S for 1-2 minutes to access the transfer ofproteins onto the nitrocellulose membrane. Rinse the blot in water to remove excess stain and mark the lane locationsand locations of molecular weight markers using a pencil.
. Rinse the blot in TBS for approximately 5 minutes.
. Block the membrane using 5 % BSA in TBS + Tween, 1 hour at RT.
. Rinse the membrane in dH2O and then wash the membrane in wash buffer [TBS + 0.1 % Tween] 3 times for 10minutes each.
. Dilute the rabbit anti-Tyro3 primary antibody in blocking buffer and incubate 1 hour at roomtemperature.
. Rinse the membrane in dH2O and then wash the membrane in wash buffer [TBS + 0.1 % Tween] 3 times for 10minutes each.
. Apply the diluted rabbit-IgG HRP-conjugated secondary antibody in blocking buffer (as per manufacturersinstructions) and incubate 1 hour at room temperature.
. Wash the blot in wash buffer [TBS + 0.1 % Tween] 3 times for 10 minutes each (this step can be repeated asrequired to reduce background).
. Apply the detection reagent of choice in accordance with the manufacturers instructions (Pierce ECL).Note: Tween-20 can be added to the blocking or antibody dilution buffer at a final concentration of 0.05-0.2 %, providedit does not interfere with antibody-antigen binding.(c) 2009 Novus Biologicals - Tyro3 Antibody - Page 1
限制 仅限研究用
状态 Liquid
浓度 1.19 mg/mL
缓冲液 PBS and 30 % Glycerol
Buffer contains: 0.1 % Sodium Azide
储存液 Sodium azide
注意事项 This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
注意事项 Avoid freeze-thaw cycles
储存条件 4 °C,-20 °C
储存方法 Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
厂商提供的图像
Image no. 1 for anti-TYRO3 Protein Tyrosine Kinase (TYRO3) (AA 100-250), (Internal Region) antibody (ABIN440474) Immunocytochemistry/Immunofluorescence: Dtk/TYRO3 Antibody [NBP1-28635] - Staining of...
有引用在: Carestia, Mena, Olexen, Ortiz Wilczyñski, Negrotto, Errasti, Gómez, Jenne, Carrera Silva, Schattner: "Platelets Promote Macrophage Polarization toward Pro-inflammatory Phenotype and Increase Survival of Septic Mice." in: Cell reports, Vol. 28, Issue 4, pp. 896-908.e5, 2019 (PubMed).

Chan, Carrera Silva, De Kouchkovsky, Joannas, Hao, Hu, Huntsman, Eng, Licona-Limón, Weinstein, Herbert, Craft, Flavell, Repetto, Correale, Burchard, Torgerson, Ghosh, Rothlin: "The TAM family receptor tyrosine kinase TYRO3 is a negative regulator of type 2 immunity." in: Science (New York, N.Y.), Vol. 352, Issue 6281, pp. 99-103, 2016 (PubMed). Further details: Flow Cytometry