HMGN1 抗体 (pSer20, pSer24)
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- 抗原 See all HMGN1 抗体
- HMGN1 (High Mobility Group Nucleosome Binding Domain 1 (HMGN1))
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抗原表位
- AA 19-28, pSer20, pSer24
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适用
- 人, 小鼠, 大鼠, Cow, Pig, 犬, 小鸡, Orang-Utan, 非洲爪蟾
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宿主
- 兔
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克隆类型
- 多克隆
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标记
- This HMGN1 antibody is un-conjugated
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应用范围
- Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)
- 特异性
- This antibody is directed against phosphorylated human HMGN protein at pS20 and pS24 residues (other HMGN proteins have this conserved sequence but at other residue positions). Reactivity is seen against HMGN1 and HMGN2. Reactivity with non-phosphorylated human HMGNs is minimal.
- 交叉反应 （详细）
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Species reactivity (expected):Xenopus, Chicken, Mouse, Bovine, Canine, Orangutan, Rat, Porcine.
Species reactivity (tested):Human. - 纯化方法
- Affinity chromatography
- 免疫原
- Synthetic peptide corresponding to amino acids 19-28 of human HMGN protein
- 亚型
- IgG
- Top Product
- Discover our top product HMGN1 Primary Antibody
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anti-High Mobility Group Nucleosome Binding Domain 1 (HMGN1) antibody
HMGN1 适用: 小鼠, 大鼠 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-High Mobility Group Nucleosome Binding Domain 1 (HMGN1) antibodyHMGN1 适用: 小鼠, 大鼠 IHC 宿主: 兔 Polyclonal unconjugated
anti-High Mobility Group Nucleosome Binding Domain 1 (HMGN1) (AA 1-96) antibodyHMGN1 适用: 小鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-High Mobility Group Nucleosome Binding Domain 1 (HMGN1) (AA 36-85) antibodyHMGN1 适用: 人, 小鼠, 大鼠, Cow, 马, 猴 WB, IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-High Mobility Group Nucleosome Binding Domain 1 (HMGN1) (AA 1-100) antibodyHMGN1 适用: 人 WB, IF 宿主: 兔 Polyclonal unconjugated
anti-High Mobility Group Nucleosome Binding Domain 1 (HMGN1) (C-Term) antibodyHMGN1 适用: 人 WB, IP 宿主: 兔 Polyclonal unconjugated
anti-High Mobility Group Nucleosome Binding Domain 1 (HMGN1) (C-Term) antibodyHMGN1 适用: 人, Cow, Pig, 马, 猴 WB, IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-High Mobility Group Nucleosome Binding Domain 1 (HMGN1) (AA 19-28), (pSer20), (pSer24) antibodyHMGN1 适用: 人 WB, IHC, ELISA 宿主: 兔 Polyclonal unconjugated
anti-High Mobility Group Nucleosome Binding Domain 1 (HMGN1) (Middle Region) antibodyHMGN1 适用: 人, 小鼠, 大鼠, Cow, Pig, 犬, 马, 兔, 豚鼠 WB 宿主: 兔 Polyclonal unconjugated
anti-High Mobility Group Nucleosome Binding Domain 1 (HMGN1) (Middle Region) antibodyHMGN1 适用: 人, 小鼠, 大鼠, Cow, Pig, 犬, 马, 兔, 豚鼠 WB, IHC 宿主: 兔 Polyclonal unconjugated
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- 应用备注
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ELISA: 1/5,000 - 1/25,000. Western Blot: 1/500 - 1/2,000. Immunohistochemistry.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. - 限制
- 仅限研究用
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- 浓度
- 1.20 mg/mL (by UV absorbance at 280 nm)
- 缓冲液
- 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 containing 0.01 % (w/v) Sodium Azide
- 储存液
- Sodium azide
- 注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 注意事项
- Avoid repeated freezing and thawing.
- 储存条件
- -20 °C
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- 抗原
- HMGN1 (High Mobility Group Nucleosome Binding Domain 1 (HMGN1))
- 别名
- HMGN1 (HMGN1 产品)
- 别名
- HMG14 antibody, hmg14 antibody, hmgn1 antibody, hmgn1b antibody, HMG-14 antibody, Hmg14 antibody, high mobility group nucleosome binding domain 1 antibody, high mobility group nucleosome binding domain 1 S homeolog antibody, high mobility group nucleosomal binding domain 1 antibody, non-histone chromosomal protein HMG-14 antibody, HMGN1 antibody, hmgn1.S antibody, hmgn1 antibody, Hmgn1 antibody, LOC785479 antibody
- 背景
- HMGNs are proteins that bind chromatin effectively reducing the compaction of the chromatin fiber and enhancing access to DNA regulatory sequences. Members of this family have a conserved chromatin binding domain which is phosphorylated during mitosis. The sequence immunized is conserved in several species. As such, this reagent is designed as a universal reagent for the detection of all phosphorylated HMGN proteins. The High Mobility Group (HMG) proteins were originally isolated from mammalian cells and were named according to their electrophoretic mobility in polyacrylamide gels. HMGs were arbitrarily classed as a specific type of nonhistone proteins based on the observation that they are ubiquitous to mammalian cells, that they share certain physical properties, and that they are associated with isolated chromatin. HMG proteins and are now subdivided into 3 families: the HMGB (formerly HMG-1/-2) family, the HMGN (formerly HMG-14/-17) family, and the HMGA (formerly HMG-I/Y/C) family. Each HMG family has a characteristic functional sequence motif. The functional motif of the HMGB family is called the HMG-box, that of the HMGN family, the nucleosomal binding domain, and that of the HMGA family, the AThook. The functional motifs characteristic of these canonical HMGs are widespread among nuclear proteins in a variety of organisms. Proteins containing any of these functional motifs embedded in their sequence are known as HMG motif proteins.Synonyms: HMG-14, HMG-17, HMG14, HMG17, HMGN1, HMGN2, Non-histone chromosomal protein HMG
- 基因ID
- 3150
- NCBI登录号
- NP_004956
- UniProt
- P05114
- 途径
- Chromatin Binding
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