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RFP 抗体 (HRP)

RFP 适用: Discosoma WB, ELISA, IHC (fro) 宿主: 兔 Polyclonal HRP
产品编号 ABIN401228
发货至: 中国
  • 抗原 See all RFP 抗体
    RFP (Red Fluorescent Protein (RFP))
    适用
    • 52
    • 1
    Discosoma
    宿主
    • 23
    • 14
    • 9
    • 5
    • 3
    克隆类型
    • 31
    • 23
    多克隆
    标记
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    • 1
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    • 1
    • 1
    • 1
    • 1
    • 1
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    This RFP antibody is conjugated to HRP
    应用范围
    • 28
    • 22
    • 15
    • 12
    • 10
    • 8
    • 6
    • 6
    • 2
    • 1
    • 1
    • 1
    Western Blotting (WB), ELISA, Immunohistochemistry (Frozen Sections) (IHC (fro))
    交叉反应 (详细)
    Ads. to Hu, Ms, Rt Serum Proteins
    纯化方法
    Immunoaffinity Chromatography using Red Fluorescent Protein (Discosoma) coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
    免疫原
    Red Fluorescent Protein (RFP) fusion protein corresponding to the full length amino acid sequence (234aa) derived from the mushroom polyp coral Discosoma
    亚型
    IgG
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  • 应用备注
    ELISA: 1: 10,000 - 1: 50,000. Western blot: 1: 1,000 - 1: 5,000. Immunohistochemistry on frozen sections.
    Other applications not tested.
    Optimal dilutions are dependent on conditions and should be determined by the user.
    限制
    仅限研究用
  • 溶解方式
    Restore with 0.1 mL of deionized water (or equivalent).
    浓度
    1.0 mg/mL
    缓冲液
    0.02 M Potassium Phosphate, 0.12 M Sodium Chloride, pH 7.2 containing 10 mg/mL BSA (Immunoglobulin and Protease free) as stabilizer and 0.01 % (w/v) Gentamicin Sulfate as preservative
    储存液
    Gentamicin sulfate
    注意事项
    This product contains Gentamicin sulfate: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.
    注意事项
    Avoid repeated freezing and thawing. Do Not Add Sodium Azide
    储存条件
    4 °C/-20 °C
    储存方法
    Store vial at 2-8 °C prior to restoration. Following restoration product can be stored undiluted at 2-8 °C for up to one month or (in aliquots) at-20 °C or below. Centrifuge product if not completely clear after standing at room temperature.
  • 抗原
    RFP (Red Fluorescent Protein (RFP))
    Abstract
    RFP 产品
    背景
    Epitope tagging is a powerful and versatile strategy for detecting and purifying proteins expressed by cloned genes. To utilize this feature, protein expression vectors are typically engineered with a nucleotide sequence that encodes the peptide epitope tag. The gene of interest is cloned in-frame relative to the tag and, upon expression, the protein of interest is synthesized as a fusion protein with the peptide tag. Fusion protein detection and/or purification is mediated by highly specific antibodies to the engineered peptide, thus eliminating the need for antibodies to proteins from each newly cloned gene. Commonly used epitope tags include glutathione-S-transferase (GST), c-myc, 6-histidine (6X-His), FLAG, green fluorescent protein (GFP), red fluorescent protein (RFP, DSRed), maltose binding protein (MBP), influenza A virus haemagglutinin (HA), b-galactosidase, and GAL4.Synonyms: DsRed Tag, Red fluorescent protein Tag
    基因ID
    86600
    UniProt
    Q9U6Y8
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