(2 references)-
- 抗原 See all FITC products
- FITC (Fluorescein Isothiocyanate (FITC))
- 适用
- 请咨询
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宿主
- 山羊
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克隆类型
- 多克隆
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标记
- This FITC antibody is conjugated to HRP
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应用范围
- Western Blotting (WB), Immunohistochemistry (Frozen Sections) (IHC (fro)), Enzyme Immunoassay (EIA), Dot Blot (DB)
- 纯化方法
- Immunoaffinity Chromatography
- 免疫原
- Fluorescein conjugated to Goat IgG
- 亚型
- IgG
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anti-Fluorescein Isothiocyanate (FITC) antibody
FITC ELISA, WB, IHC, FACS 宿主: 绵羊 Polyclonal unconjugated
anti-Fluorescein Isothiocyanate (FITC) antibody (HRP)FITC ELISA, WB, IHC 宿主: 绵羊 Polyclonal HRP
anti-Fluorescein Isothiocyanate (FITC) antibody (Alkaline Phosphatase (AP))FITC ELISA, WB, IHC 宿主: 绵羊 Polyclonal Alkaline Phosphatase (AP)
anti-Fluorescein Isothiocyanate (FITC) antibody (Biotin)FITC ELISA, WB, IHC, FACS 宿主: 绵羊 Polyclonal Biotin
anti-Fluorescein Isothiocyanate (FITC) antibody (PE)FITC ELISA, FACS 宿主: 绵羊 Polyclonal PE
anti-Fluorescein Isothiocyanate (FITC) antibodyFITC WB 宿主: 兔 Polyclonal unconjugated
anti-Fluorescein Isothiocyanate (FITC) antibodyFITC 适用: 人 WB 宿主: 兔 Monoclonal unconjugated
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- 应用备注
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Suitable for immunoblotting (western or dot blot), ELISA, immunoperoxidase electronmicroscopy and immunohistochemistry as well as other peroxidase-antibody basedenzymatic assays requiring lot-to-lot consistency. Recommended Dilutions: ELISA: 1/10,000-1/50,000. Western Blot: 1/2,000-1/5,000. Immunohistochemistry: 1/500-1/2,000.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. - 限制
- 仅限研究用
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- 溶解方式
- Restore with 1.0 mL of deionized water (or equivalent).
- 浓度
- 1.0 mg/mL (by UV absorbance at 280 nm)
- 缓冲液
- 0.02 M Potassium Phosphate, 0.12 M Sodium Chloride, pH 7.2 containing 10 mg/mL BSA (IgG and Protease Free) as stabilizer and 0.01 % (w/v) Gentamicin Sulfate as preservative
- 储存液
- Gentamicin sulfate
- 注意事项
- This product contains Gentamicin sulfate: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.
- 注意事项
- Avoid repeated freezing and thawing. Do Not Add Sodium Azide
- 储存条件
- 4 °C/-20 °C
- 储存方法
- Prior to reconstitution store at 2-8 °C. Following reconstitution store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.
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: "New synthetic strategy for diporphyrins: pinacol coupling-rearrangement." in: Chemistry (Weinheim an der Bergstrasse, Germany), Vol. 17, Issue 26, pp. 7154-7, (2011) (PubMed).
: "Specific penetration and accumulation of a homing peptide within atherosclerotic plaques of apolipoprotein E-deficient mice." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 108, Issue 17, pp. 7154-9, (2011) (PubMed).
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: "New synthetic strategy for diporphyrins: pinacol coupling-rearrangement." in: Chemistry (Weinheim an der Bergstrasse, Germany), Vol. 17, Issue 26, pp. 7154-7, (2011) (PubMed).
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- 抗原
- FITC (Fluorescein Isothiocyanate (FITC))
- 别名
- FITC (FITC 产品)
- 物质类
- Chemical
- 背景
- Fluorescein is a fluorophore commonly used to label proteins - protein-fluorescein conjugates are not usually susceptible to precipitation. In addition to its relatively high absorptivity, excellent fluorescence quantum yield and good water solubility, fluorescein has an excitation maximum of 494 nm that closely matches the 488 nm spectral line of the argon-ion laser, making it an important fluorophore for confocal laser-scanning microscopy and flow cytometry applications. Its fluorescence is? pH sensitive and is significantly reduced below? pH 7. Fluorescein emits most strongly between 500 and 550 nm, but it has a relatively broad emission spectrum reaching to over 600 nm. Several derivatives of fluorescein are commonly used, including FITC (fluorescein isothiocyanate), carboxylates and succinimidyl esters.Synonyms: Fluorescein Isothiocyanate
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