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DYKDDDDK Tag 抗体

WB, IP, FACS, EIA 宿主: 小鼠 Monoclonal 29E4-G7-H11 unconjugated
产品编号 ABIN400789
发货至: 中国
  • 抗原 See all DYKDDDDK Tag products
    DYKDDDDK Tag
    适用
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    宿主
    • 58
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    小鼠
    克隆类型
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    单克隆
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    This DYKDDDDK Tag antibody is un-conjugated
    应用范围
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    Western Blotting (WB), Immunoprecipitation (IP), Flow Cytometry (FACS), Enzyme Immunoassay (EIA)
    序列
    DYKDDDDK
    产品特性
    Synonyms: DYKDDDDK Epitope Tag, ECS Epitope Tag, DYKDDDDK-Tag, ECS-tag, D-tag
    纯化方法
    Protein A Chromatography followed by extensive dialysis against the buffer.
    免疫原
    Synthetic peptide corresponding to the DYKDDDDK epitope tag peptide DYKDDDDK(Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys) conjugated to KLH using maleimide. Remarks: Residues of Glycine and Cysteine were added to the termini to facilitatecoupling.
    克隆位点
    29E4-G7-H11
    亚型
    IgG2a
  • 应用备注
    This antibody is optimally suited for monitoring the expression of DYKDDDDK tagged fusionproteins. As such, this antibody can be used to identify fusion proteins containing theDYKDDDDK epitope. The antibody recognizes the epitope tag fused to either the amino- orcarboxy- termini of targeted proteins. The epitope tag peptide sequence was first derivedfrom the 11-amino-acid leader peptide of the gene-10 product from bacteriophage T7. DYKDDDDK is the most commonly used hydrophilic octapeptide tag. Recommended Dilutions:
    限制
    仅限研究用
  • 状态
    Liquid
    浓度
    1.0 mg/mL (by UV absorbance at 280 nm)
    缓冲液
    0.02 M Potassium Phosphate, 0.12 M Sodium Chloride, pH 7.2 containing 0.01 % (w/v) Sodium Azide as preservative.
    储存液
    Sodium azide
    注意事项
    This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    储存条件
    4 °C/-20 °C
    储存方法
    Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing.
    Shelf life: one year from despatch.
    有效期
    12 months
  • 抗原
    DYKDDDDK Tag
    别名
    DYKDDDDK Epitope Tag (DYKDDDDK Tag 产品)
    物质类
    Tag
    背景
    Sequences that are easily recognized by tag-specific antibodies. Due to their small size, epitope tags do not affect the tagged protein's biochemical properties. Most often sequences encoding the epitope tag are included with target DNA at the time of cloning to produce fusion proteins containing the epitope tag sequence. This allows anti-epitope tag antibodies to serve as universal detection reagents for any tag containing protein produced by recombinant means. This means that anti-epitope tag antibodies are a useful alternative to generating specific antibodies to identify, immunoprecipitate or immunoaffinity purify a recombinant protein. The anti-epitope tag antibody is usually functional in a variety of antibody-dependent experimental procedures. Expression vectors producing epitope tag fusion proteins are available for a variety of host expression systems including bacteria, yeast, insect and mammalian cells.
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