山羊 anti-小鼠 IgG (Heavy & Light Chain) Antibody (HRP)

ABIN398387 产品详细信息, 供应商: Log in to see
抗原
抗原表位
Heavy & Light Chain
6236
2031
1776
1135
782
648
372
305
34
34
31
15
12
12
12
12
11
7
3
3
1
1
1
1
1
1
1
适用
小鼠
3083
2914
2384
1663
1509
786
737
489
476
411
401
395
342
298
271
160
159
111
55
34
30
26
16
15
12
10
8
7
5
5
4
4
3
3
3
3
2
2
2
2
2
2
1
1
1
1
1
1
1
宿主
山羊
7135
5288
1973
1079
507
362
89
84
51
35
23
15
8
5
3
1
1
克隆类型
多克隆
标记
HRP
2226
1823
1751
1266
794
718
430
343
235
147
145
140
140
116
113
112
102
98
94
93
92
91
89
84
81
80
78
75
73
72
68
68
65
61
55
54
54
54
54
53
53
53
53
53
53
52
48
43
41
37
32
31
23
23
22
20
19
18
17
16
15
15
15
14
14
14
12
12
12
12
12
11
11
10
10
10
10
10
9
9
9
9
9
8
8
8
8
8
7
6
6
6
6
6
6
6
6
5
5
5
5
5
5
5
5
4
4
4
4
3
3
3
3
3
3
3
3
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
应用范围
ELISA, Western Blotting (WB)
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免疫原 Full length mouse IgG (purified)
亚型 IgG
产品特性 This polyclonal antibody is highly purified from goat antiserum by immunoaffinity chromatography and then conjugated to horseradish peroxidase. It reacts with mouse IgG heavy and light chains.
纯化方法 Immunoaffinity chromatography
抗原
应用备注 Antibodies-Online Goat Anti-Mouse IgG (H&L) [HRP] is suitable for immunoblotting (western and dot blots), ELISA, immunoperoxidase electron microscopy, immunohistochemistry, and other peroxidase-antibody based enzymatic assays requiring lot-to-lot consistency.The investigator must determine the ideal working concentration for each specific application. The ideal working concentration must take into account such factors as secondary antibody affinity, antigen concentration, sensitivity of the detection method, temperature, and the length of the incubations. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.ELISA: 1:5,000-1:20,000
Western blot: 1:1,000-1:10,000Other applications: user-optimized
限制 仅限研究用
状态 Lyophilized
溶解方式 Reconstitute the lyophilized antibody with deionized water (or equivalent) to a final concentration of 1.0 mg/ml.
浓度 1 mg/mL
缓冲液 PBS, pH 7.4, containing 1 % BSA and 0.01 % Thimerosal
储存液 Thimerosal (Merthiolate)
注意事项 Biohazard Informations: This product contains thimerosal which is hazardous.
注意事项 Avoid repeated freezing and thawing. Dilute only prior to immediate use
Centrifuge product if not completely clear after standing at room temperature.
Do NOT add Sodium Azide! Use of Sodium Azide will inhibit enzyme activity of horseradish peroxidase.
储存条件 4 °C/-20 °C
储存方法 Goat Anti-Mouse IgG (H&L) [HRP] Polyclonal Antibody is stable for 2-3 weeks at 2-8 °C. For long term storage, aliquot and store at -28 °C or below.
有效期 3 weeks
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Chen, Hwang, Chan, Chung, Wang, Li, Ma, Lin, Hsieh, Chang, Kung, Wu, Chu, Tai, Gao, Zheng, Yokoyama, Austyn, Lin: "Binding of HIV-1 gp120 to DC-SIGN promotes ASK-1-dependent activation-induced apoptosis of human dendritic cells." in: PLoS pathogens, Vol. 9, Issue 1, pp. e1003100, 2013 (PubMed).

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Zullo, Lee: "Mycobacterial induction of autophagy varies by species and occurs independently of mammalian target of rapamycin inhibition." in: The Journal of biological chemistry, Vol. 287, Issue 16, pp. 12668-78, 2012 (PubMed).

Gao, Tang, Li, Huan: "Specific inhibition of AQP1 water channels in human pulmonary microvascular endothelial cells by small interfering RNAs." in: The journal of trauma and acute care surgery, Vol. 72, Issue 1, pp. 150-61, 2012 (PubMed).

Shi, Zhang, Qu, Ding, Zheng: "Genomic organization, promoter characterization and expression analysis of the leukocyte cell-derived chemotaxin-2 gene in Epinephelus akaraa." in: Fish & shellfish immunology, Vol. 32, Issue 6, pp. 1041-50, 2012 (PubMed).

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Gao, Li, Huan, Li: "Caveolin-1 siRNA increases the pulmonary microvascular and alveolar epithelial permeability in rats." in: The Journal of trauma, Vol. 70, Issue 1, pp. 210-9, 2011 (PubMed).

Fan, Cai, Zeng, Hao, Giancotti, Fu: "Integrin ?4 signaling promotes mammary tumor cell adhesion to brain microvascular endothelium by inducing ErbB2-mediated secretion of VEGF." in: Annals of biomedical engineering, Vol. 39, Issue 8, pp. 2223-41, 2011 (PubMed).

Yu, Xu, Ren, Xu, Yuan, Qi, Tian: "Preventive action of curcumin in experimental acute pancreatitis in mouse." in: The Indian journal of medical research, Vol. 134, Issue 5, pp. 717-24, 2011 (PubMed).

Lü, Chen, Yang, Chen, Zhou, Xu, Li, Han, Wang: "The effects of proteasome inhibitor bortezomib on a P-gp positive leukemia cell line K562/A02." in: International journal of laboratory hematology, Vol. 32, Issue 1 Pt 1, pp. e123-31, 2010 (PubMed).