(19 references)-
- 抗原 See all IgG products
- IgG
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抗原表位
- Heavy & Light Chain
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适用
- 小鼠
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宿主
- 山羊
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克隆类型
- 多克隆
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标记
- HRP
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应用范围
- ELISA, Western Blotting (WB)
- 产品特性
- This polyclonal antibody is highly purified from goat antiserum by immunoaffinity chromatography and then conjugated to horseradish peroxidase. It reacts with mouse IgG heavy and light chains.
- 纯化方法
- Immunoaffinity chromatography
- 免疫原
- Full length mouse IgG (purified)
- 亚型
- IgG
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Goat anti-Mouse IgG antibody (DyLight 488) - Preadsorbed
WB, FLISA, FM Polyclonal IgG DyLight 488
Goat anti-Mouse IgG (Heavy & Light Chain) antibody (HRP) - PreadsorbedELISA, IHC, WB, DB, IEM Polyclonal IgG HRP
Rabbit anti-Mouse IgG antibody (Atto 550) - PreadsorbedWB, FLISA, FM Polyclonal IgG Atto 550
Goat anti-Mouse IgG (Heavy & Light Chain) antibody (Atto 594) - PreadsorbedWB, FLISA, FM Polyclonal IgG Atto 594
Goat anti-Mouse IgG (Heavy & Light Chain) antibody (Atto 425) - PreadsorbedWB, FLISA, FM Polyclonal IgG Atto 425
Goat anti-Mouse IgG (Heavy & Light Chain) antibody (Atto 647N) - PreadsorbedWB, FLISA, FM Polyclonal IgG Atto 647N
Goat anti-Mouse IgG (Heavy & Light Chain) antibody - PreadsorbedELISA, IHC, WB Polyclonal IgG unconjugated Fab fragment
Goat anti-Mouse IgG (Heavy & Light Chain) antibody (HRP)ELISA, IHC, WB, DB, IEM Polyclonal IgG HRP
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- 应用备注
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Antibodies-Online Goat Anti-Mouse IgG (H&L) [HRP] is suitable for immunoblotting (western and dot blots), ELISA, immunoperoxidase electron microscopy, immunohistochemistry, and other peroxidase-antibody based enzymatic assays requiring lot-to-lot consistency.The investigator must determine the ideal working concentration for each specific application. The ideal working concentration must take into account such factors as secondary antibody affinity, antigen concentration, sensitivity of the detection method, temperature, and the length of the incubations. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.ELISA: 1:5,000-1:20,000
Western blot: 1:1,000-1:10,000Other applications: user-optimized - 限制
- 仅限研究用
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- 状态
- Lyophilized
- 溶解方式
- Reconstitute the lyophilized antibody with deionized water (or equivalent) to a final concentration of 1.0 mg/ml.
- 浓度
- 1 mg/mL
- 缓冲液
- PBS, pH 7.4, containing 1 % BSA and 0.01 % Thimerosal
- 储存液
- Thimerosal (Merthiolate)
- 注意事项
- Biohazard Informations: This product contains thimerosal which is hazardous.
- 注意事项
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Avoid repeated freezing and thawing. Dilute only prior to immediate use
Centrifuge product if not completely clear after standing at room temperature.
Do NOT add Sodium Azide! Use of Sodium Azide will inhibit enzyme activity of horseradish peroxidase. - 储存条件
- 4 °C/-20 °C
- 储存方法
- Goat Anti-Mouse IgG (H&L) [HRP] Polyclonal Antibody is stable for 2-3 weeks at 2-8 °C. For long term storage, aliquot and store at -28 °C or below.
- 有效期
- 3 weeks
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: "Cranberry extract standardized for proanthocyanidins promotes the immune response of Caenorhabditis elegans to Vibrio cholerae through the p38 MAPK pathway and HSF-1." in: PLoS ONE, Vol. 9, Issue 7, pp. e103290, (2014) (PubMed).
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: "Photo-induced inhibition of Alzheimer's ?-amyloid aggregation in vitro by rose bengal." in: Biomaterials, Vol. 38, pp. 43-9, (2014) (PubMed).
: "Binding of HIV-1 gp120 to DC-SIGN promotes ASK-1-dependent activation-induced apoptosis of human dendritic cells." in: PLoS pathogens, Vol. 9, Issue 1, pp. e1003100, (2013) (PubMed).
: "Intranasal immunization with a non-adjuvanted adhesive protein descended from Pasteurella pneumotropica and its preventive efficacy against opportunistic infection in mice." in: Vaccine, Vol. 31, Issue 48, pp. 5729-35, (2013) (PubMed).
: "Mycobacterial induction of autophagy varies by species and occurs independently of mammalian target of rapamycin inhibition." in: The Journal of biological chemistry, Vol. 287, Issue 16, pp. 12668-78, (2012) (PubMed).
: "Specific inhibition of AQP1 water channels in human pulmonary microvascular endothelial cells by small interfering RNAs." in: The journal of trauma and acute care surgery, Vol. 72, Issue 1, pp. 150-61, (2012) (PubMed).
: "Genomic organization, promoter characterization and expression analysis of the leukocyte cell-derived chemotaxin-2 gene in Epinephelus akaraa." in: Fish & shellfish immunology, Vol. 32, Issue 6, pp. 1041-50, (2012) (PubMed).
: "Expression and characterization of recombinant soluble porcine CD3 ectodomain molecules: mapping the epitope of an anti-porcine CD3 monoclonal antibody 898H2-6-15." in: Cellular immunology, Vol. 276, Issue 1-2, pp. 162-7, (2012) (PubMed).
: "Dystroglycan is associated to the disulfide isomerase ERp57." in: Experimental cell research, Vol. 318, Issue 19, pp. 2460-9, (2012) (PubMed).
: "Selection of single chain variable fragments specific for the human-inducible costimulator using ribosome display." in: Applied biochemistry and biotechnology, Vol. 168, Issue 5, pp. 967-79, (2012) (PubMed).
: "Caveolin-1 siRNA increases the pulmonary microvascular and alveolar epithelial permeability in rats." in: The Journal of trauma, Vol. 70, Issue 1, pp. 210-9, (2011) (PubMed).
: "Integrin ?4 signaling promotes mammary tumor cell adhesion to brain microvascular endothelium by inducing ErbB2-mediated secretion of VEGF." in: Annals of biomedical engineering, Vol. 39, Issue 8, pp. 2223-41, (2011) (PubMed).
: "Preventive action of curcumin in experimental acute pancreatitis in mouse." in: The Indian journal of medical research, Vol. 134, Issue 5, pp. 717-24, (2011) (PubMed).
: "The effects of proteasome inhibitor bortezomib on a P-gp positive leukemia cell line K562/A02." in: International journal of laboratory hematology, Vol. 32, Issue 1 Pt 1, pp. e123-31, (2010) (PubMed).
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: "MnTM-4-PyP modulates endogenous antioxidant responses and protects primary cortical neurons against oxidative stress." in: CNS neuroscience & therapeutics, Vol. 21, Issue 5, pp. 435-45, (2015) (PubMed).
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- 抗原
- IgG
- Abstract
- IgG 产品
- 物质类
- Antibody
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