GTP Cyclohydrolase 1 (GCH1) (AA 84-173) 抗体

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抗原
  • DYT14
  • DYT5
  • DYT5a
  • GCH
  • GTP-CH-1
  • GTPCH1
  • HPABH4B
  • GTP-CH
  • GTPCH
  • Gch
  • MGC89622
  • gch1a
  • wu:fc16b10
  • zgc:195269
  • GTP cyclohydrolase 1
  • GTP cyclohydrolase 1-like
  • GCH1
  • Gch1
  • gch1
  • Weevi_1190
  • Halhy_0838
  • Sinme_1220
  • Sphch_1077
  • LOC100136794
抗原表位
AA 84-173
15
11
10
9
7
7
5
3
2
2
2
1
1
1
1
适用

82
32
31
5
5
4
3
3
2
2
2
2
2
1
1
1
宿主
小鼠
56
17
11
克隆类型 (克隆位点)
单克隆 ()
标记
非结合性
7
6
4
3
3
3
1
1
1
1
1
1
1
1
1
1
1
应用范围
ELISA, Immunohistochemistry (IHC), Immunoprecipitation (IP), Western Blotting (WB)
68
49
27
13
8
7
7
1
1
选项
Supplier
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Supplier Product No.
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免疫原

Antigen Type: Recombinant Protein

Target/Specificity: GCH1 (NP_000152, 84 a.a. ~ 172 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.

克隆位点 4A12
亚型 IgG2a kappa
特异性 GCH1 (NP_000152, 84 a.a. ~ 173 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
纯化方法 This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
别名 GCH1 (GCH1 Antibody 摘要)
背景

Other Names: GTP cyclohydrolase 1, GTP cyclohydrolase I, GTP-CH-I, GCH1, DYT5, GCH

Gene Description: GTP cyclohydrolase 1

Gene Summary: This gene encodes a member of the GTP cyclohydrolase family. The encoded protein is the first and rate-limiting enzyme in tetrahydrobiopterin (BH4) biosynthesis, catalyzing the conversion of GTP into 7,8-dihydroneopterin triphosphate. BH4 is an essential cofactor required by aromatic amino acid hydroxylases as well as nitric oxide synthases. Mutations in this gene are associated with malignant hyperphenylalaninemia and dopa-responsive dystonia. Several alternatively spliced transcript variants encoding different isoforms have been described, however, not all variants give rise to a functional enzyme. [provided by RefSeq

分子量 27903 DA
基因ID 2643
NCBI登录号 NP_000152, NP_001019195, NP_001019241, NP_001019242
UniProt P30793
应用备注 Western blot = 1:500-1000
说明

Background: This gene encodes a member of the GTP cyclohydrolase family. The encoded protein is the first and rate-limiting enzyme in tetrahydrobiopterin (BH4) biosynthesis, catalyzing the conversion of GTP into 7,8-dihydroneopterin triphosphate. BH4 is an essential cofactor required by aromatic amino acid hydroxylases as well as nitric oxide synthases. Mutations in this gene are associated with malignant hyperphenylalaninemia and dopa-responsive dystonia. Several alternatively spliced transcript variants encoding different isoforms have been described, however, not all variants give rise to a functional enzyme.

限制 仅限研究用
浓度 1 mg/mL
缓冲液 Clear, colorless solution in phosphate buffered saline,  pH 7.2 .
储存条件 4 °C/-20 °C
储存方法 Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
有效期 6 months
厂商提供的图像
Western Blotting (WB) image for anti-GTP Cyclohydrolase 1 (GCH1) (AA 84-173) antibody (ABIN396170) GCH1 monoclonal antibody (M01), clone 4A12 Western Blot analysis of GCH1 expression i...
Immunohistochemistry (IHC) image for anti-GTP Cyclohydrolase 1 (GCH1) (AA 84-173) antibody (ABIN396170) anti-GTP Cyclohydrolase 1 (GCH1) (AA 84-173) antibody (Image 2)
Western Blotting (WB) image for anti-GTP Cyclohydrolase 1 (GCH1) (AA 84-173) antibody (ABIN396170) Antibody Reactive Against Recombinant Protein.Western Blot detection against Immunoge...
Immunohistochemistry (IHC) image for anti-GTP Cyclohydrolase 1 (GCH1) (AA 84-173) antibody (ABIN396170) Immunoperoxidase of monoclonal antibody to GCH1 on formalin-fixed paraffin-embedded h...
Immunoprecipitation (IP) image for anti-GTP Cyclohydrolase 1 (GCH1) (AA 84-173) antibody (ABIN396170) Immunoprecipitation of GCH1 transfected lysate using anti-GCH1 monoclonal antibody an...
ELISA image for anti-GTP Cyclohydrolase 1 (GCH1) (AA 84-173) antibody (ABIN396170) Detection limit for recombinant GST tagged GCH1 is approximately 0.3ng/ml as a captur...
背景 Dabo, Grönbladh, Nyberg, Sundström-Poromaa, Akerud: "Different SNP combinations in the GCH1 gene and use of labor analgesia." in: Molecular pain, Vol. 6, pp. 41, 2010 (PubMed).

Bailey, Xie, Do, Montpetit, Diaz, Mohan, Keavney, Yusuf, Gerstein, Engert, Anand: "Variation at the NFATC2 locus increases the risk of thiazolidinedione-induced edema in the Diabetes REduction Assessment with ramipril and rosiglitazone Medication (DREAM) study." in: Diabetes Care, Vol. 33, Issue 10, pp. 2250-3, 2010 (PubMed).

Tu, Ding, Wang, Xu, Zhang, Huang, Wang: "Exploring epistatic relationships of NO biosynthesis pathway genes in susceptibility to CHD." in: Acta pharmacologica Sinica, Vol. 31, Issue 7, pp. 874-80, 2010 (PubMed).

Cao, Zheng, Tang, Xiao, Zhang, Tang, He, Wang, Ding, Chen: "Four novel mutations in the GCH1 gene of Chinese patients with dopa-responsive dystonia." in: Movement disorders : official journal of the Movement Disorder Society, Vol. 25, Issue 6, pp. 755-60, 2010 (PubMed).

Ionova, Vásquez-Vivar, Cooley, Khanna, Whitsett, Herrnreiter, Migrino, Ge, Regner, Channon, Alp, Pieper: "Cardiac myocyte-specific overexpression of human GTP cyclohydrolase I protects against acute cardiac allograft rejection." in: American journal of physiology. Heart and circulatory physiology, Vol. 299, Issue 1, pp. H88-96, 2010 (PubMed).

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