小鼠 anti-人 IgE (Fc Region) Antibody
(1 reference)-
- 抗原 See all IgE products
- IgE
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抗原表位
- Fc Region
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适用
- 人
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宿主
- 小鼠
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克隆类型
- 单克隆
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应用范围
- ELISA, Western Blotting (WB), Flow Cytometry (FACS), Functional Studies (Func)
- 特异性
- Human IgE, Fce3-4, no cross-ractivity with other human or murine Ab isotypes
- 产品特性
- IGHE, chromosome 14q32.33
- 纯化方法
- Affinity - purified from cell culture supernatant by Immunoaffinity Chromatography on CH-Sepharose 4B
- 纯度
- > 95 %, tested by analytical Gel Permeation Chromatography
- 过滤
- Sterile filtered
- 免疫原
- purified human myeloma IgE
- 克隆位点
- BSW17-NBS01
- 亚型
- IgG1 kappa
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Mouse anti-Human IgE (Fc Region) antibody (Biotin)
ELISA Monoclonal HP6029 IgG2a Biotin
Mouse anti-Human IgE antibody (FITC)FACS Monoclonal BE5 IgG1 FITC
Mouse anti-Human IgE antibody (PE)FACS Monoclonal BE5 IgG1 PE
Mouse anti-Human IgE (Fc Region) antibodyELISA, WB, FACS, Func Monoclonal Le27-NBS01 IgG1 kappa unconjugated
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- 应用备注
- For functional cellular assays (mast cell / basophil activation), when used at dilutions < 1:100, the sodium azide free formulation is recommended. Quality assurance: Reactivity > 99 %, compared to calibrator (Internal Standard mAb BSW17), tested by ELISA binding to immobilized human Standard IgE (SUS-11)
- 限制
- 仅限研究用
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- 浓度
- 1 mg/mL
- 缓冲液
- PBS, pH=7.2, sterile filtered, contains no NaN3
- 储存液
- Azide free
- 注意事项
- Monoclonal antibodies should not be stored at a temperature below -25 °C due to the aggregation effect of the protein.
- 储存条件
- 4 °C
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: "Molecular basis for nonanaphylactogenicity of a monoclonal anti-IgE antibody." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 165, Issue 2, pp. 813-9, (2000) (PubMed).
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: "Molecular basis for nonanaphylactogenicity of a monoclonal anti-IgE antibody." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 165, Issue 2, pp. 813-9, (2000) (PubMed).
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- 抗原
- IgE
- Abstract
- IgE 产品
- 背景
- The antibody recognizes a conformational epitope within the Ce3 domain of human IgE spanning towards the amino-terminal part of the Ce4 domain. It blocks the binding of IgE to its high affinity receptor on mast cells and basophils. It is therefore suited as an inhibitory reference compound for mast cell modulator profiling and basic research on IgE mediated mast cell receptor signaling.
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