ATP6V0D2 抗体 (Isoform 1)
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- 抗原 See all ATP6V0D2 抗体
- ATP6V0D2 (ATPase, H+ Transporting, Lysosomal 38kDa, V0 Subunit D2 (ATP6V0D2))
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抗原表位
- Isoform 1
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适用
- Tomato, Arabidopsis thaliana, Nicotiana tabacum
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宿主
- 山羊
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克隆类型
- 多克隆
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标记
- This ATP6V0D2 antibody is un-conjugated
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应用范围
- Western Blotting (WB)
- 交叉反应 （详细）
- Specificity has been determined using ELISA competition experiments using conjugate of 5-Methoxytryptamine-Glutaric anhydride Compounds Cross-reactivity ratio (a) 5-Methoxytryptamine-S-BSA: 1, Free melatonin 1/ 130, 5-Hydroxytryptamine-A-BSA 1/ 50 000, DL 5-Methoxytryptophan-SA-BSA 1/>50000, Tryptamine-SA-BSA 1/>20 000 (a) : 5-Methoxytryptamine-GA-BSA concentration/ conjugated close structure concentration at half displacement.
- 产品特性
- Expected / apparent Molecular Weight of the Antigene: 26 / 31 kDa (Arabidopsis thaliana)
- 纯化方法
- serum
- 免疫原
- KLH-conjugated synthetic peptide chosen from subunit E of plant V-ATPase including Arabidopsis thaliana At4g11150. Peptide is conserved in vacuolar H+-ATPase subunit E, isoform 1 to 3 (VHA-E1).
- Top Product
- Discover our top product ATP6V0D2 Primary Antibody
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anti-ATPase, H+ Transporting, Lysosomal 38kDa, V0 Subunit D2 (ATP6V0D2) (AA 238-306) antibody
ATP6V0D2 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 7A4 unconjugated
anti-ATPase, H+ Transporting, Lysosomal 38kDa, V0 Subunit D2 (ATP6V0D2) (AA 238-306) antibodyATP6V0D2 适用: 人 WB, ELISA 宿主: 小鼠 Polyclonal unconjugated
anti-ATPase, H+ Transporting, Lysosomal 38kDa, V0 Subunit D2 (ATP6V0D2) (Isoform 1) antibodyATP6V0D2 适用: Tomato, Arabidopsis thaliana, Zea mays, Pteris vittata (Chinese ladder brake), Oryza sativa, Lilium longiflorum (Trumpet lily), Chlamydomonas reinhardtii, Barley WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-ATPase, H+ Transporting, Lysosomal 38kDa, V0 Subunit D2 (ATP6V0D2) (AA 251-300) antibodyATP6V0D2 适用: 人, 大鼠, 小鼠, 兔, 豚鼠, 犬, Cow, Pig WB 宿主: 兔 Polyclonal unconjugated
anti-ATPase, H+ Transporting, Lysosomal 38kDa, V0 Subunit D2 (ATP6V0D2) (C-Term) antibodyATP6V0D2 适用: 人, 大鼠, 小鼠, 兔, 豚鼠, 犬, Pig, Bat, 猴 WB 宿主: 兔 Polyclonal unconjugated
anti-ATPase, H+ Transporting, Lysosomal 38kDa, V0 Subunit D2 (ATP6V0D2) (Middle Region) antibodyATP6V0D2 适用: 人, 大鼠, 小鼠, 兔, 豚鼠, 犬, Cow, 马, Pig WB 宿主: 兔 Polyclonal unconjugated
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- 应用备注
- Recommended Dilution: 1 : 1 000 with alkaline phosphatase or 1 : 3000 with ECL (WB).
- 说明
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V-ATPase is very sensitive for the redox of the SDS buffer. We recommend using at least 50-100 mM DTT freshly prepared before handling the sample.2 hours incubation with primary antibody is recommended over over night incubation which can contribute to increased background.
- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 溶解方式
- For reconstitution add 300 µL of sterile water.
- 注意事项
- Once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
- 储存条件
- -20 °C
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- 抗原
- ATP6V0D2 (ATPase, H+ Transporting, Lysosomal 38kDa, V0 Subunit D2 (ATP6V0D2))
- 别名
- V-ATPase (ATP6V0D2 产品)
- 别名
- ATP6D2 antibody, VMA6 antibody, AI324824 antibody, V-ATPase antibody, 1620401A02Rik antibody, ATPase H+ transporting V0 subunit d2 antibody, ATPase, H+ transporting, lysosomal V0 subunit D2 antibody, ATPase H+ transporting V0 subunit D2 antibody, ATPase, H+ transporting, lysosomal 38kDa, V0 subunit d2 L homeolog antibody, ATP6V0D2 antibody, Atp6v0d2 antibody, atp6v0d2.L antibody
- 背景
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AGI Code: At4g11150
Plant vacuole V-ATPase is responsible for energization of transport of ions and metabolites, and acts as well 'house-keeping' and as a stress response enzyme. V-ATPase is a multi-subunit enzyme composed of a membrane sector and a cytosolic catalytic sector. It is related to the FoF1 ATP synthase. Alternative protein names: Vacuolar proton pump subunit E, Protein EMBRYO DEFECTIVE 2448 - 分子量
- expected: 26 kDa, apparent: 31 kDa (Arabidopsis thaliana)
- UniProt
- Q39258
- 途径
- Transition Metal Ion Homeostasis, Proton Transport
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