V5 Epitope Tag 抗体
-
- 抗原 See all V5 Epitope Tag products
- V5 Epitope Tag
- 适用
- 请咨询
-
宿主
- 兔
-
克隆类型
- 多克隆
-
标记
- This V5 Epitope Tag antibody is un-conjugated
-
应用范围
- Western Blotting (WB), Enzyme Immunoassay (EIA)
- 序列
- GKPIPNPLLG LDST
- 纯化方法
- Protein G Chromatography, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
- 免疫原
- KLH conjugated synthetic peptide encoding the V5 tag (GKPIPNPLLGLDST) was used as antigen.
- 亚型
- Ig Fraction
-
-
- 应用备注
-
ELISA: 1/1,000. Western Blot: 1/100-1/500.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. - 限制
- 仅限研究用
-
- 状态
- Liquid
- 浓度
- 0.25 mg/mL
- 缓冲液
- PBS containing 0.09 % (w/v) Sodium Azide as preservative.
- 储存液
- Sodium azide
- 注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 注意事项
- Avoid repeated freezing and thawing.
- 储存条件
- 4 °C/-20 °C
- 储存方法
- Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.
-
- 抗原
- V5 Epitope Tag
- 别名
- Pk (V5) Epitope Tag (GKPIPNPLLGLDST) (V5 Epitope Tag 产品)
- 物质类
- Tag
- 背景
- Epitope tags consisting of short sequences recognized by well-characterizated antibodies have been widely used in the study of protein expression in various systems. The V5 epitope tag is is composed of a 14 residue peptide, GKPIPNPLLGLDST, derived from a small epitope (Pk) present on the P and V proteins of the paramyxovirus of simian virus 5 (SV5). This polyclonal antibody provides a simple solution to detect the expression of a V5-tagged protein in cells.
-