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V5 Epitope Tag 抗体

WB, EIA 宿主: 兔 Polyclonal unconjugated
产品编号 ABIN356537
发货至: 中国
  • 抗原 See all V5 Epitope Tag products
    V5 Epitope Tag
    适用
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    宿主
    • 59
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    克隆类型
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    多克隆
    标记
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    This V5 Epitope Tag antibody is un-conjugated
    应用范围
    • 100
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    Western Blotting (WB), Enzyme Immunoassay (EIA)
    序列
    GKPIPNPLLG LDST
    纯化方法
    Protein G Chromatography, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
    免疫原
    KLH conjugated synthetic peptide encoding the V5 tag (GKPIPNPLLGLDST) was used as antigen.
    亚型
    Ig Fraction
  • 应用备注
    ELISA: 1/1,000. Western Blot: 1/100-1/500.
    Other applications not tested.
    Optimal dilutions are dependent on conditions and should be determined by the user.
    限制
    仅限研究用
  • 状态
    Liquid
    浓度
    0.25 mg/mL
    缓冲液
    PBS containing 0.09 % (w/v) Sodium Azide as preservative.
    储存液
    Sodium azide
    注意事项
    This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    注意事项
    Avoid repeated freezing and thawing.
    储存条件
    4 °C/-20 °C
    储存方法
    Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.
  • 抗原
    V5 Epitope Tag
    别名
    Pk (V5) Epitope Tag (GKPIPNPLLGLDST) (V5 Epitope Tag 产品)
    物质类
    Tag
    背景
    Epitope tags consisting of short sequences recognized by well-characterizated antibodies have been widely used in the study of protein expression in various systems. The V5 epitope tag is is composed of a 14 residue peptide, GKPIPNPLLGLDST, derived from a small epitope (Pk) present on the P and V proteins of the paramyxovirus of simian virus 5 (SV5). This polyclonal antibody provides a simple solution to detect the expression of a V5-tagged protein in cells.
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